sp. nov., isolated from a tomato plant ( L.) Free

Abstract

A Gram-stain-positive, rod-shaped, endospore-forming bacterium (designated strain CC-Bw-5) was isolated from chopped tomato stems. The isolate grew at 20–40 °C, pH 6.0–8.0 and tolerated 6  % (w/v) NaCl. The most closely related strains in terms of 16S rRNA gene sequence similarity were (95.3  %) and (95.3  %). The G+C content of the genomic DNA was 37.2 ± 3.6 mol%. Strain CC-Bw-5 was determined to possess C, iso-C, iso-C, anteiso-C and iso-C as predominant fatty acids. The polar lipid profile consisted of predominant amounts of diphosphatidylglycerol, and moderate-to-trace amounts of phosphatidylethanolamine, phosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified phosphoglycolipid. The cell-wall peptidoglycan contained -diaminopimelic acid; menaquinone (MK-7) was the predominant respiratory quinone. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-Bw-5 is proposed to represent a novel species within the genus for which the name sp. nov. is proposed. The type strain is CC-Bw-5 ( = BCRC 80623 = JCM 19140).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000223
2015-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/7/2085.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000223&mimeType=html&fmt=ahah

References

  1. Albert R.A., Archambault J., Rosselló-Mora R., Tindall B.J., Matheny M. ( 2005;). Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55 21252130 [View Article] [PubMed].
    [Google Scholar]
  2. Albuquerque L., Tiago I., Taborda M., Nobre M.F., Veríssimo A., da Costa M.S. ( 2008;). Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond. Int J Syst Evol Microbiol 58 226230 [View Article] [PubMed].
    [Google Scholar]
  3. Choi J.-H., Cha C.-J. ( 2014;). Bacillus panacisoli sp. nov., isolated from ginseng soil. Int J Syst Evol Microbiol 64 901906 [View Article] [PubMed].
    [Google Scholar]
  4. Collins M.D. ( 1985;). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. London: Academic Press;.
    [Google Scholar]
  5. Denizci A.A., Kazan D., Erarslan A. ( 2010;). Bacillus marmarensis sp. nov., an alkaliphilic, protease-producing bacterium isolated from mushroom compost. Int J Syst Evol Microbiol 60 15901594 [View Article] [PubMed].
    [Google Scholar]
  6. Dittmer J.C., Lester R.L. ( 1964;). A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 5 126127 [PubMed].
    [Google Scholar]
  7. Edwards U., Rogall T., Blöcker H., Emde M., Böttger E.C. ( 1989;). Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17 78437853 [View Article] [PubMed].
    [Google Scholar]
  8. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  9. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  10. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  11. Heiner C.R., Hunkapiller L.K., Chen S.M., Glass J.I., Chen E.Y. ( 1998;). Sequencing multimegabase-template DNA using BigDye terminator chemistry. Genome Res 8 557561.
    [Google Scholar]
  12. Heyndrickx M., Coorevits A., Scheldeman P., Lebbe L., Schumann P., Rodríguez-Diaz M., Forsyth G., Dinsdale A., Heyrman J., other authors. ( 2012;). Emended descriptions of Bacillus sporothermodurans Bacillus oleronius with the inclusion of dairy farm isolates of both species. Int J Syst Evol Microbiol 62 307314 [View Article] [PubMed].
    [Google Scholar]
  13. Jacin H., Mishkin A.R. ( 1965;). Separation of carbohydrates on borate-impregnated silica gel G plates. J Chromatogr A 18 170173 [View Article] [PubMed].
    [Google Scholar]
  14. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  15. Lee G.-H., Rhee M.-S., Chang D.-H., Kwon K.K., Bae K.S., Yang S.-H., Kim B.-C. ( 2014;). Bacillus solimangrovi sp. nov., isolated from mangrove soil. Int J Syst Evol Microbiol 64 16221628 [View Article] [PubMed].
    [Google Scholar]
  16. Logan N.A., Berge O., Bishop A.H., Busse H.J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59 21142121 [View Article] [PubMed].
    [Google Scholar]
  17. Manickam N., Singh N.K., Bajaj A., Kumar R.M., Kaur G., Kaur N., Bala M., Kumar A., Mayilraj S. ( 2014;). Bacillus mesophilum sp. nov., strain IITR-54T, a novel 4-chlorobiphenyl dechlorinating bacterium. Arch Microbiol 196 517523 [View Article] [PubMed].
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39 159167 [View Article].
    [Google Scholar]
  19. Miller L.T. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16 584586 [PubMed].
    [Google Scholar]
  20. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  21. Murray R.G.E., Doetsch R.N., Robinow C.F. ( 1994;). Determination and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 2141. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  22. Paisley R. ( 1996). MIS Whole Cell Fatty Acid Analysis by Gas Chromatography Training Manual Newark, DE: MIDI;.
    [Google Scholar]
  23. Pappa A., Sánchez-Porro C., Lazoura P., Kallimanis A., Perisynakis A., Ventosa A., Drainas C., Koukkou A.I. ( 2010;). Bacillus halochares sp. nov., a halophilic bacterium isolated from a solar saltern. Int J Syst Evol Microbiol 60 14321436 [View Article] [PubMed].
    [Google Scholar]
  24. Priest F.G., Goodfellow M., Todd C. ( 1988;). A numerical classification of the genus Bacillus . J Gen Microbiol 134 18471882 [PubMed].
    [Google Scholar]
  25. Rhuland L.E., Work E., Denman R., Hoare D. ( 1955;). The behavior of the isomers of diaminopimelic acid on paper chromatograms. J Am Chem Soc 77 48444846 [View Article].
    [Google Scholar]
  26. Ross H.N.M., Grant W.D., Harris J.E. ( 1985;). Lipids in archaebacterial taxonomy. . In Chemical Methods in Bacterial Systematics, pp. 289300. Edited by Goodfellow M., Minnikin D. E. London: Academic Press;.
    [Google Scholar]
  27. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  28. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  29. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849 [View Article].
    [Google Scholar]
  30. Subhash Y., Sasikala Ch., Ramana ChV. ( 2014;). Bacillus luteus sp. nov., isolated from soil. Int J Syst Evol Microbiol 64 15801586 [View Article] [PubMed].
    [Google Scholar]
  31. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  32. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed].
    [Google Scholar]
  33. Tiago I., Mendes V., Pires C., Morais P.V., Veríssimo A. ( 2006;). Chimaereicella alkaliphila gen. nov., sp. nov., a Gram-negative alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 29 100108 [View Article] [PubMed].
    [Google Scholar]
  34. Tindall B.J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60 249266 [View Article] [PubMed].
    [Google Scholar]
  35. Xue Y., Ventosa A., Wang X., Ren P., Zhou P., Ma Y. ( 2008;). Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China. Int J Syst Evol Microbiol 58 28282832 [View Article] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000223
Loading
/content/journal/ijsem/10.1099/ijs.0.000223
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed