1887

Abstract

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium (FJAT-14571) was isolated from a soil sample in Taiwan. Strain FJAT-14571 grew at 20–40 °C (optimum 35 °C), pH 6–10 (optimum pH 8) and 0–2 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FJAT-14571 was a member of the genus and was most closely related to DSM 24771 (96.2 %). DNA–DNA relatedness between strain FJAT-14571 and DSM 24771 was low (32.0 % ± 0.88 %). The diagnostic diamino acid of the peptidoglycan of strain FJAT-14571 was -diaminopimelic acid and the predominant menaquinone was MK-7 (96.6 %). The major cellular fatty acids were iso-C (46.4 %), anteiso-C (7.6 %), iso-C (8.2 %) and iso-C (10.0 %) and the DNA G+C content was 40.8 mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that strain FJAT-14571 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is FJAT-14571 ( = DSM 27845 = CGMCC1.1 2698).

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2015-07-01
2019-10-14
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References

  1. Albert R.A., Archambault J., Rosselló-Mora R., Tindall B.J., Matheny M.. ( 2005;). Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55: 2125–2130 [CrossRef] [PubMed].
    [Google Scholar]
  2. Albuquerque L., Tiago I., Taborda M., Nobre M.F., Veríssimo A., da Costa M.S.. ( 2008;). Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond. Int J Syst Evol Microbiol 58: 226–230 [CrossRef] [PubMed].
    [Google Scholar]
  3. Atlas R.M.. ( 1993;). Handbook of Microbiological Media. .. Edited by Parks L. C.. Boca Raton, FL: CRC Press;.
  4. Chandna P., Mayilraj S., Kuhad R.C.. ( 2013;). Bacillus paraflexus sp. nov., isolated from compost. Int J Syst Evol Microbiol 63: 4735–4743 [CrossRef] [PubMed].
    [Google Scholar]
  5. Chen Y.G., Cui X.L., Pukall R., Li H.M., Yang Y.L., Xu L.H., Wen M.L., Peng Q., Jiang C.L.. ( 2007;). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57: 2327–2332 [CrossRef] [PubMed].
    [Google Scholar]
  6. Chou J.H., Chou Y.J., Lin K.Y., Sheu S.Y., Sheu D.S., Arun A.B., Young C.C., Chen W.M.. ( 2007;). Paenibacillus fonticola sp. nov., isolated from a warm spring. Int J Syst Evol Microbiol 57: 1346–1350 [CrossRef] [PubMed].
    [Google Scholar]
  7. Cohn F.. ( 1872;). Untersuchungen über Bakterien. Beitrage zur Biologie der Pflanzen 1: ((Heft 2)), 127–224.
    [Google Scholar]
  8. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E.. ( 1977;). Distribution of menaquinones in Actinomycetes Corynebacteria. J Gen Microbiol 100: 221–230 [CrossRef] [PubMed].
    [Google Scholar]
  9. Cowan S.T., Steel K.J.. ( 1965;). Manual for the Identification of Medical Bacteria London: Cambridge University Press;.
    [Google Scholar]
  10. de Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133–142 [CrossRef] [PubMed].
    [Google Scholar]
  11. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  12. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  13. Fitch W.M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  14. Gregersen T.. ( 1978;). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5: 123–127 [CrossRef].
    [Google Scholar]
  15. Groth I., Schumann P., Weiss N., Martin K., Rainey F.A.. ( 1996;). Agrococcus jenensis gen. nov. sp. nov., a new genus of Actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46: 234–239 [CrossRef] [PubMed].
    [Google Scholar]
  16. Heyrman J., Rodríguez-Díaz M., Devos J., Felske A., Logan N.A., De Vos P.. ( 2005;). Bacillus arenosi sp. nov. Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. Int J Syst Evol Microbiol 55: 111–117 [CrossRef] [PubMed].
    [Google Scholar]
  17. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H..), ( 1985;). Genetic Manipulation of Streptomyces. A Laboratory Manual., Norwich: John Innes Foundation;.
    [Google Scholar]
  18. Huss V.A., Festl H., Schleifer K.H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4: 184–192 [CrossRef] [PubMed].
    [Google Scholar]
  19. Jukes T.H., Cantor C.R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–132. Edited by Munro H. N..3 New York: Academic Press; [CrossRef]
    [Google Scholar]
  20. Kämpfer P.. ( 1994;). Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. Syst Appl Microbiol 17: 86–98 [CrossRef].
    [Google Scholar]
  21. Kämpfer P., Arun A.B., Busse H.J., Langer S., Young C.C., Chen W.M., Syed A.A., Rekha P.D.. ( 2011;). Virgibacillus soli sp. nov. isolated from mountain soil. Int J Syst Evol Microbiol 61: 275–280 [CrossRef] [PubMed].
    [Google Scholar]
  22. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  23. Lee F.L., Kuo H.P., Tai C.J., Yokota A., Lo C.C.. ( 2007;). Paenibacillus taiwanensis sp. nov. isolated from soil in Taiwan. Int J Syst Evol Microbiol 57: 1351–1354 [CrossRef] [PubMed].
    [Google Scholar]
  24. Lee F.L., Tien C.J., Tai C.J., Wang L.T., Liu Y.C., Chern L.L.. ( 2008;). Paenibacillus taichungensis sp. nov. from soil in Taiwan. Int J Syst Evol Microbiol 58: 2640–2645 [CrossRef] [PubMed].
    [Google Scholar]
  25. Liu B., Liu G.H., Hu G.H., Chen M.C.. ( 2014a;). Bacillus mesonae sp. nov. isolated from the root of Mesona chinensis. Int J Syst Evol Microbiol 64: 3346–3352 [CrossRef] [PubMed].
    [Google Scholar]
  26. Liu B., Liu G.H., Sengonca C., Schumann P., Wang M.K., Tang J.Y., Chen M.C.. ( 2014b;). Bacillus cihuensis sp. nov. isolated from rhizosphere soil of a plant in the Cihu area of Taiwan. Antonie van Leeuwenhoek 106: 1147–1155 [CrossRef] [PubMed].
    [Google Scholar]
  27. Logan N.A., De Vos P.. ( 2009;). Genus I. Bacillus. . In Bergey's Manual of Systematic Bacteriology, pp. 21–128. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B..3 New York: Springer;.
    [Google Scholar]
  28. Logan N.A., Lebbe L., Verhelst A., Goris J., Forsyth G., Rodríguez-Díaz M., Heyndrickx M., De Vos P.. ( 2004;). Bacillus shackletonii sp. nov. from volcanic soil on Candlemas Island, South Sandwich archipelago. Int J Syst Evol Microbiol 54: 373–376 [CrossRef] [PubMed].
    [Google Scholar]
  29. Logan N.A., Berge O., Bishop A.H., Busse H.J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59: 2114–2121 [CrossRef] [PubMed].
    [Google Scholar]
  30. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118 [CrossRef] [PubMed].
    [Google Scholar]
  31. Minnikin D.E., Collins M.D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas. Oerskovia and related taxa. J Appl Bacteriol 47: 87–95 [CrossRef].
    [Google Scholar]
  32. Murray R.G.E., Doetsch R.N., Robinow C.F.. ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  33. Priest F.G., Goodfellow M., Todd C.. ( 1988;). A numerical classification of the genus Bacillus. J Gen Microbiol 134: 1847–1882 [PubMed].
    [Google Scholar]
  34. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  35. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC News 20: 16.
    [Google Scholar]
  36. Schleifer K.H.. ( 1985;). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18: 123–156 [CrossRef].
    [Google Scholar]
  37. Seiler H., Wenning M., Schmidt V., Scherer S.. ( 2013;). Bacillus gottheilii sp. nov. isolated from a pharmaceutical manufacturing site. Int J Syst Evol Microbiol 63: 867–872 [CrossRef] [PubMed].
    [Google Scholar]
  38. Sheu S.Y., Arun A.B., Jiang S.R., Young C.C., Chen W.M.. ( 2011;). Allobacillus halotolerans gen. nov. sp. nov. isolated from shrimp paste. Int J Syst Evol Microbiol 61: 1023–1027 [CrossRef] [PubMed].
    [Google Scholar]
  39. Smibert R.M., Krieg N.R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  40. Stackebrandt E., Goebel B.M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44: 846–849 [CrossRef].
    [Google Scholar]
  41. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  42. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  43. Wang C.Y., Chang C.C., Ng C.C., Chen T.W., Shyu Y.T.. ( 2008;). Virgibacillus chiguensis sp. nov. a novel halophilic bacterium isolated from Chigu, a previously commercial saltern located in southern Taiwan. Int J Syst Evol Microbiol 58: 341–345 [CrossRef] [PubMed].
    [Google Scholar]
  44. Wayne L.G.. ( 1988;). International Committee on Systematic Bacteriology: announcement of the report of the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Zentralbl Bakteriol Mikrobiol Hyg A. 268: 433–434 [PubMed].
    [Google Scholar]
  45. Zhang J., Wang J., Fang C., Song F., Xin Y., Qu L., Ding K.. ( 2010;). Bacillus oceanisediminis sp. nov. isolated from marine sediment. Int J Syst Evol Microbiol 60: 2924–2929 [CrossRef] [PubMed].
    [Google Scholar]
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