1887

Abstract

Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus by using a repetitive sequence-based PCR fingerprinting with the (GTG) primer. Phylogenetic analysis based on 16S rRNA gene, , , , and sequences showed that the group is closely related to but separated from the three hitherto known subspecies, subsp. , subsp. and subsp. . Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA–DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within , for which the name subsp subsp. nov. is proposed. The type strain is NRL/St 12/356 ( = CCM 8529 = LMG 28327).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000220
2015-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/7/2071.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000220&mimeType=html&fmt=ahah

References

  1. Bergeron M., Dauwalder O., Gouy M., Freydiere A.M., Bes M., Meugnier H., Benito Y., Etienne J., Lina G., other authors. ( 2011;). Species identification of staphylococci by amplification and sequencing of the tuf gene compared to the gap gene and by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Eur J Clin Microbiol Infect Dis 30 343354 [View Article] [PubMed].
    [Google Scholar]
  2. Cleenwerck I., Vandemeulebroecke K., Janssens D., Swings J. ( 2002;). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. Int J Syst Evol Microbiol 52 15511558 [View Article] [PubMed].
    [Google Scholar]
  3. De Bel A., Van Hoorde K., Wybo I., Vandoorslaer K., Echahidi F., De Brandt E., Schumann P., Ieven M., Soetens O., other authors. ( 2013;). Staphylococcus jettensis sp. nov., a coagulase-negative staphylococcal species isolated from human clinical specimens. Int J Syst Evol Microbiol 63 32503256 [View Article] [PubMed].
    [Google Scholar]
  4. De Bel A., Švec P., Petráš P., Sedláček I., Pantu˚ček R., Echahidi F., Piérard D., Vandamme P. ( 2014;). Reclassification of Staphylococcus jettensis De Bel et al. 2013 as Staphylococcus petrasii subsp. jettensis subsp. nov. and emended description of Staphylococcus petrasii Pantucek et al. 2013. Int J Syst Evol Microbiol 64 41984201 [View Article] [PubMed].
    [Google Scholar]
  5. EUCAST ( 2014;). European Committee on Antimicrobial Susceptibility Testing. EUCAST Clinical breakpoints–bacteria (version 4.0; http://www.eucast.org). .
  6. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [View Article].
    [Google Scholar]
  7. Freney J., Kloos W.E., Hajek V., Webster J.A., Bes M., Brun Y., Vernozy-Rozand C. ( 1999;). Recommended minimal standards for description of new staphylococcal species. Int J Syst Bacteriol 49 489502 [View Article] [PubMed].
    [Google Scholar]
  8. Gevers D., Huys G., Swings J. ( 2001;). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205 3136 [View Article] [PubMed].
    [Google Scholar]
  9. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. ( 1998;). Evaluation of a microplate DNA–DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44 11481153 [View Article].
    [Google Scholar]
  10. Kotilainen P., Huovinen P., Eerola E. ( 1991;). Application of gas-liquid chromatographic analysis of cellular fatty acids for species identification and typing of coagulase-negative staphylococci. J Clin Microbiol 29 315322 [PubMed].
    [Google Scholar]
  11. Kwok A.Y., Chow A.W. ( 2003;). Phylogenetic study of Staphylococcus Macrococcus species based on partial hsp60 gene sequences. Int J Syst Evol Microbiol 53 8792 [View Article] [PubMed].
    [Google Scholar]
  12. Mellmann A., Becker K., von Eiff C., Keckevoet U., Schumann P., Harmsen D. ( 2006;). Sequencing and staphylococci identification. Emerg Infect Dis 12 333336 [View Article] [PubMed].
    [Google Scholar]
  13. Mesbah M., Whitman W.B. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479 297306 [View Article] [PubMed].
    [Google Scholar]
  14. Pantu˚ček R., Sedláček I., Petráš P., Koukalová D., Švec P., Šteˇtina V., Vancanneyt M., Chrastinová L., Vokurková J., other authors. ( 2005;). Staphylococcus simiae sp. nov., isolated from South American squirrel monkeys. Int J Syst Evol Microbiol 55 19531958 [View Article] [PubMed].
    [Google Scholar]
  15. Pantu˚ček R., Švec P., Dajcs J.J., Machová I., Černohlávková J., Šedo O., Gelbíčová T., Mašlanˇová I., Doškarˇ J., other authors. ( 2013;). Staphylococcus petrasii sp. nov. including S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov., isolated from human clinical specimens and human ear infections. Syst Appl Microbiol 36 9095 [View Article] [PubMed].
    [Google Scholar]
  16. Schleifer K.H., Bell J.A. ( 2009;). Genus I. Staphylococcus Rosenbach 1884, 18AL (Nom. Cons. Opin. 17 Jud. Comm. 1958, 153.). . In Bergey's Manual of Systematic Bacteriology, pp. 392421. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. vol. 3 New York: Springer;.
    [Google Scholar]
  17. Shah M.M., Iihara H., Noda M., Song S.X., Nhung P.H., Ohkusu K., Kawamura Y., Ezaki T. ( 2007;). dnaJ gene sequence-based assay for species identification and phylogenetic grouping in the genus Staphylococcus . Int J Syst Evol Microbiol 57 2530 [View Article] [PubMed].
    [Google Scholar]
  18. Supré K., De Vliegher S., Cleenwerck I., Engelbeen K., Van Trappen S., Piepers S., Sampimon O.C., Zadoks R.N., De Vos P., Haesebrouck F. ( 2010;). Staphylococcus devriesei sp. nov., isolated from teat apices and milk of dairy cows. Int J Syst Evol Microbiol 60 27392744 [View Article] [PubMed].
    [Google Scholar]
  19. Švec P., Pantu˚ček R., Petráš P., Sedláček I., Nováková D. ( 2010;). Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting. Syst Appl Microbiol 33 451456 [View Article] [PubMed].
    [Google Scholar]
  20. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  21. Yugueros J., Temprano A., Berzal B., Sánchez M., Hernanz C., Luengo J.M., Naharro G. ( 2000;). Glyceraldehyde-3-phosphate dehydrogenase-encoding gene as a useful taxonomic tool for Staphylococcus spp. J Clin Microbiol 38 43514355 [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000220
Loading
/content/journal/ijsem/10.1099/ijs.0.000220
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error