1887

Abstract

Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus by using a repetitive sequence-based PCR fingerprinting with the (GTG) primer. Phylogenetic analysis based on 16S rRNA gene, , , , and sequences showed that the group is closely related to but separated from the three hitherto known subspecies, subsp. , subsp. and subsp. . Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA–DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within , for which the name subsp subsp. nov. is proposed. The type strain is NRL/St 12/356 ( = CCM 8529 = LMG 28327).

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2015-07-01
2019-10-20
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