1887

Abstract

A novel Gram-stain-negative, rod-shaped, motile bacterium, designated strain AK21, was isolated from coastal surface sea water at Visakhapatnam, India. The strain was positive for oxidase, catalase, lipase, -proline arylamidase and tyrosine arylamidase activities. The predominant fatty acids were C, C 3-OH, C, Cω9, Cω9 and summed feature 3 (Cω7 and/or iso-C 2-OH). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, two unidentified phospholipids and one unidentified lipid. Q-10 was the predominant respiratory quinone. The DNA G+C content of the strain was 54.6 mol%. 16S rRNA gene sequence analysis indicated that strain AK21 was a member of the genus and was closely related to , with pairwise sequence similarity of 97.2 % to the type strain, with similarity to other members of the genus of 94.0–96.8 %. The mean DNA–DNA relatedness of strain AK21 with JCM 17469 was 34.5 %, and relatedness with JCM 15154 was 40.5 %. Phylogenetic analysis showed that strain AK21 clustered with the type strains of and at distances of 2.9 and 2.8 % (97.1 and 97.2 % similarity), respectively. Based on the phenotypic characteristics and on phylogenetic inference, it appears that strain AK21 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is AK21 ( = MTCC 11704 = JCM 18428).

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2015-07-01
2020-01-21
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