1887

Abstract

A novel Gram-stain-negative, rod-shaped, motile bacterium, designated strain AK21, was isolated from coastal surface sea water at Visakhapatnam, India. The strain was positive for oxidase, catalase, lipase, -proline arylamidase and tyrosine arylamidase activities. The predominant fatty acids were C, C 3-OH, C, Cω9, Cω9 and summed feature 3 (Cω7 and/or iso-C 2-OH). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, two unidentified phospholipids and one unidentified lipid. Q-10 was the predominant respiratory quinone. The DNA G+C content of the strain was 54.6 mol%. 16S rRNA gene sequence analysis indicated that strain AK21 was a member of the genus and was closely related to , with pairwise sequence similarity of 97.2 % to the type strain, with similarity to other members of the genus of 94.0–96.8 %. The mean DNA–DNA relatedness of strain AK21 with JCM 17469 was 34.5 %, and relatedness with JCM 15154 was 40.5 %. Phylogenetic analysis showed that strain AK21 clustered with the type strains of and at distances of 2.9 and 2.8 % (97.1 and 97.2 % similarity), respectively. Based on the phenotypic characteristics and on phylogenetic inference, it appears that strain AK21 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is AK21 ( = MTCC 11704 = JCM 18428).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000218
2015-07-01
2021-03-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/7/2056.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000218&mimeType=html&fmt=ahah

References

  1. Aguilera M., Jiménez-Pranteda M.L., Kharroub K., González-Paredes A., Durban J.J., Russell N.J., Ramos-Cormenzana A., Monteoliva-Sánchez M. ( 2009;). Marinobacter lacisalsi sp. nov., a moderately halophilic bacterium isolated from the saline-wetland wildfowl reserve Fuente de Piedra in southern Spain. Int J Syst Evol Microbiol 59 16911695. [CrossRef]
    [Google Scholar]
  2. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. ( 1990;). Basic local alignment search tool. J Mol Biol 215 403410. [CrossRef]
    [Google Scholar]
  3. Anil Kumar P., Aravind R., Francis K., Bhumika V., Ritika C., Priyashanth P., Srinivas T.N.R. ( 2012;). Shivajiella indica gen. nov., sp. nov., a marine bacterium of the family “Cyclobacteriaceae” with nitrate reducing activity. Syst Appl Microbiol 35 320325. [CrossRef]
    [Google Scholar]
  4. Antunes A., França L., Rainey F.A., Huber R., Nobre M.F., Edwards K.J., da Costa M.S. ( 2007;). Marinobacter salsuginis sp. nov., isolated from the brine–seawater interface of the Shaban Deep, Red Sea. Int J Syst Evol Microbiol 57 10351040. [CrossRef]
    [Google Scholar]
  5. Bagheri M., Amoozegar M.A., Didari M., Makhdoumi-Kakhki A., Schumann P., Spröer C., Sánchez-Porro C., Ventosa A. ( 2013;). Marinobacter persicus sp. nov., a moderately halophilic bacterium from a saline lake in Iran. Antonie van Leeuwenhoek 104 4754. [CrossRef]
    [Google Scholar]
  6. Bligh E.G., Dyer W.J. ( 1959;). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37 911917. [CrossRef]
    [Google Scholar]
  7. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221230. [CrossRef]
    [Google Scholar]
  8. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791. [CrossRef]
    [Google Scholar]
  9. Gauthier M.J., Lafay B., Christen R., Fernandez L., Acquaviva M., Bonin P., Bertrand J.-C. ( 1992;). Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 42 568576. [CrossRef]
    [Google Scholar]
  10. Gorshkova N.M., Ivanova E.P., Sergeev A.F., Zhukova N.V., Alexeeva Y., Wright J.P., Nicolau D.V., Mikhailov V.V., Christen R. ( 2003;). Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan. Int J Syst Evol Microbiol 53 20732078. [CrossRef]
    [Google Scholar]
  11. Green D.H., Bowman J.P., Smith E.A., Gutierrez T., Bolch C.J.S. ( 2006;). Marinobacter algicola sp. nov., isolated from laboratory cultures of paralytic shellfish toxin-producing dinoflagellates. Int J Syst Evol Microbiol 56 523527. [CrossRef]
    [Google Scholar]
  12. Groth I., Schumann P., Rainey F.A., Martin K., Schuetze B., Augsten K. ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47 11291133. [CrossRef]
    [Google Scholar]
  13. Gu J., Cai H., Yu S.-L., Qu R., Yin B., Guo Y.-F., Zhao J.-Y., Wu X.-L. ( 2007;). Marinobacter gudaonensis sp. nov., isolated from an oil-polluted saline soil in a Chinese oilfield. Int J Syst Evol Microbiol 57 250254. [CrossRef]
    [Google Scholar]
  14. Guo B., Gu J., Ye Y.-G., Tang Y.-Q., Kida K., Wu X.-L. ( 2007;). Marinobacter segnicrescens sp. nov., a moderate halophile isolated from benthic sediment of the South China Sea. Int J Syst Evol Microbiol 57 19701974. [CrossRef]
    [Google Scholar]
  15. Handley K.M., Héry M., Lloyd J.R. ( 2009;). Marinobacter santoriniensis sp. nov., an arsenate-respiring and arsenite-oxidizing bacterium isolated from hydrothermal sediment. Int J Syst Evol Microbiol 59 886892. [CrossRef]
    [Google Scholar]
  16. Huo Y.-Y., Wang C.-S., Yang J.-Y., Wu M., Xu X.-W. ( 2008;). Marinobacter mobilis sp. nov. and Marinobacter zhejiangensis sp. nov., halophilic bacteria isolated from the East China Sea. Int J Syst Evol Microbiol 58 28852889. [CrossRef]
    [Google Scholar]
  17. Huu N.B., Denner E.B.M., Ha D.T., Wanner G., Stan-Lotter H. ( 1999;). Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well. Int J Syst Bacteriol 49 367375. [CrossRef]
    [Google Scholar]
  18. Kaeppel E.C., Gärdes A., Seebah S., Grossart H.-P., Ullrich M.S. ( 2012;). Marinobacter adhaerens sp. nov., isolated from marine aggregates formed with the diatom Thalassiosira weissflogii. Int J Syst Evol Microbiol 62 124128. [CrossRef]
    [Google Scholar]
  19. Kharroub K., Aguilera M., Jiménez-Pranteda M.L., González-Paredes A., Ramos-Cormenzana A., Monteoliva-Sánchez M. ( 2011;). Marinobacter oulmenensis sp. nov., a moderately halophilic bacterium isolated from brine of a salt concentrator. Int J Syst Evol Microbiol 61 22102214. [CrossRef]
    [Google Scholar]
  20. Kim B.-Y., Weon H.-Y., Yoo S.-H., Kim J.-S., Kwon S.-W., Stackebrandt E., Go S.-J. ( 2006;). Marinobacter koreensis sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 56 26532656. [CrossRef]
    [Google Scholar]
  21. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721. [CrossRef]
    [Google Scholar]
  22. Komagata K., Suzuki K. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19 161207. [CrossRef]
    [Google Scholar]
  23. Lányi B. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19 167. [CrossRef]
    [Google Scholar]
  24. Lee O.O., Lai P.Y., Wu H.-X., Zhou X.-J., Miao L., Wang H., Qian P.-Y. ( 2012;). Marinobacter xestospongiae sp. nov., isolated from the marine sponge Xestospongia testudinaria collected from the Red Sea. Int J Syst Evol Microbiol 62 19801985. [CrossRef]
    [Google Scholar]
  25. Liebgott P.-P., Casalot L., Paillard S., Lorquin J., Labat M. ( 2006;). Marinobacter vinifirmus sp. nov., a moderately halophilic bacterium isolated from a wine-barrel-decalcification wastewater. Int J Syst Evol Microbiol 56 25112516. [CrossRef]
    [Google Scholar]
  26. Liu C., Chen C.-X., Zhang X.-Y., Yu Y., Liu A., Li G.-W., Chen X.-L., Chen B., Zhou B.-C., Zhang Y.-Z. ( 2012;). Marinobacter antarcticus sp. nov., a halotolerant bacterium isolated from Antarctic intertidal sandy sediment. Int J Syst Evol Microbiol 62 18381844. [CrossRef]
    [Google Scholar]
  27. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218. [CrossRef]
    [Google Scholar]
  28. Márquez M.C., Ventosa A. ( 2005;). Marinobacter hydrocarbonoclasticus Gauthier et al. 1992 and Marinobacter aquaeolei Nguyen et al. 1999 are heterotypic synonym. Int J Syst Evol Microbiol 55 13491351. [CrossRef]
    [Google Scholar]
  29. Martín S., Márquez M.C., Sánchez-Porro C., Mellado E., Arahal D.R., Ventosa A. ( 2003;). Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activity. Int J Syst Evol Microbiol 53 13831387. [CrossRef]
    [Google Scholar]
  30. Montes M.J., Bozal N., Mercadé E. ( 2008;). Marinobacter guineae sp. nov., a novel moderately halophilic bacterium from an Antarctic environment. Int J Syst Evol Microbiol 58 13461349. [CrossRef]
    [Google Scholar]
  31. Nei M., Kumar S. ( 2000). Molecular Evolution and Phylogenetics New York: Oxford University Press;.
    [Google Scholar]
  32. Ng H.J., López-Pérez M., Webb H.K., Gomez D., Sawabe T., Ryan J., Vyssotski M., Bizet C., Malherbe F., other authors. ( 2014;). Marinobacter salarius sp. nov. and Marinobacter similis sp. nov., isolated from sea water. PLoS One 9 e106514. [CrossRef]
    [Google Scholar]
  33. Ng H.J., López-Pérez M., Webb H.K., Gomez D., Sawabe T., Ryan J., Vyssotski M., Bizet C., Malherbe F., other authors. ( 2015;). Marinobacter salarius sp. nov. and Marinobacter similis sp. nov. In List of new names and new combinations previously effectively, but not validly, published, Validation List 161. Int J Syst Evol Microbiol 65 14. [CrossRef]
    [Google Scholar]
  34. Qu L., Zhu F., Zhang J., Gao C., Sun X. ( 2011;). Marinobacter daqiaonensis sp. nov., a moderate halophile isolated from a Yellow Sea salt pond. Int J Syst Evol Microbiol 61 30033008. [CrossRef]
    [Google Scholar]
  35. Rakshak K., Ravinder K., Nupur, Srinivas T.N.R., Anil Kumar P. ( 2013;). Caldimonas meghalayensis sp. nov., a novel thermophilic betaproteobacterium isolated from a hot spring of Meghalaya in northeast India. Antonie van Leeuwenhoek 104 12171225. [CrossRef]
    [Google Scholar]
  36. Roh S.W., Quan Z.-X., Nam Y.-D., Chang H.-W., Kim K.-H., Rhee S.-K., Oh H.-M., Jeon C.O., Yoon J.-H., Bae J.-W. ( 2008;). Marinobacter goseongensis sp. nov., from seawater. Int J Syst Evol Microbiol 58 28662870. [CrossRef]
    [Google Scholar]
  37. Romanenko L.A., Schumann P., Rohde M., Zhukova N.V., Mikhailov V.V., Stackebrandt E. ( 2005;). Marinobacter bryozoorum sp. nov. and Marinobacter sediminum sp. nov., novel bacteria from the marine environment. Int J Syst Evol Microbiol 55 143148. [CrossRef]
    [Google Scholar]
  38. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425.
    [Google Scholar]
  39. Shieh W.Y., Jean W.D., Lin Y.-T., Tseng M. ( 2003;). Marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan. Can J Microbiol 49 244252. [CrossRef]
    [Google Scholar]
  40. Shivaji S., Gupta P., Chaturvedi P., Suresh K., Delille D. ( 2005;). Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 55 14531456. [CrossRef]
    [Google Scholar]
  41. Sly L.I., Blackall L.L., Kraat P.C., Tao T.-S., Sangkhobol V. ( 1986;). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5 139156. [CrossRef]
    [Google Scholar]
  42. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  43. Srinivas T.N.R., Nageswara Rao S.S.S., Vishnu Vardhan Reddy P., Pratibha M.S., Sailaja B., Kavya B., Hara Kishore K., Begum Z., Singh S.M., Shivaji S. ( 2009;). Bacterial diversity and bioprospecting for cold-active lipases, amylases and proteases, from culturable bacteria of Kongsfjorden and Ny-Ålesund, Svalbard, Arctic. Curr Microbiol 59 537547. [CrossRef]
    [Google Scholar]
  44. Tamura K., Nei M. ( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10 512526.
    [Google Scholar]
  45. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739. [CrossRef]
    [Google Scholar]
  46. Tourova T.P., Antonov A.S. ( 1987;). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19 333355. [CrossRef]
    [Google Scholar]
  47. Vandamme P., Pot B., Gillis M., de Vos P., Kersters K., Swings J. ( 1996;). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60 407438.
    [Google Scholar]
  48. Wang C.-Y., Ng C.-C., Tzeng W.-S., Shyu Y.-T. ( 2009;). Marinobacter szutsaonensis sp. nov., isolated from a solar saltern. Int J Syst Evol Microbiol 59 26052609. [CrossRef]
    [Google Scholar]
  49. Xu X.-W., Wu Y.-H., Wang C.-S., Yang J.-Y., Oren A., Wu M. ( 2008;). Marinobacter pelagius sp. nov., a moderately halophilic bacterium. Int J Syst Evol Microbiol 58 637640. [CrossRef]
    [Google Scholar]
  50. Yoon J.-H., Shin D.-Y., Kim I.-G., Kang K.H., Park Y.-H. ( 2003;). Marinobacter litoralis sp. nov., a moderately halophilic bacterium isolated from sea water from the East Sea in Korea. Int J Syst Evol Microbiol 53 563568. [CrossRef]
    [Google Scholar]
  51. Yoon J.-H., Yeo S.-H., Kim I.-G., Oh T.-K. ( 2004;). Marinobacter flavimaris sp. nov. and Marinobacter daepoensis sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54 17991803. [CrossRef]
    [Google Scholar]
  52. Yoon J.-H., Lee M.-H., Kang S.-J., Oh T.-K. ( 2007;). Marinobacter salicampi sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 57 21022105. [CrossRef]
    [Google Scholar]
  53. Zhang D.-C., Li H.-R., Xin Y.-H., Chi Z.-M., Zhou P.-J., Yu Y. ( 2008;). Marinobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from the Arctic. Int J Syst Evol Microbiol 58 14631466. [CrossRef]
    [Google Scholar]
  54. Zhuang D.-C., Chen Y.-G., Zhang Y.-Q., Tang S.-K., Wu X.-L., Tan Z.-C., Li W.-J., Cui X.-L. ( 2009;). Marinobacter zhanjiangensis sp. nov., a marine bacterium isolated from sea water of a tidal flat of the South China Sea. Antonie van Leeuwenhoek 96 295301. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000218
Loading
/content/journal/ijsem/10.1099/ijs.0.000218
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error