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Abstract

A Gram-stain-positive, facultatively anaerobic, non-spore-forming, non-flagellated and rod-shaped or ovoid bacterial strain, designated BS-12M, was isolated from a tidal flat sediment on the South Sea, South Korea. Strain BS-12M grew optimally at 35 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain BS-12M fell within the cluster comprising the type strains of species of the genus , joining the type strain of with which it shared the highest sequence similarity (98.6 %). It exhibited 16S rRNA gene sequence similarity of 96.1–97.9 % to the type strains of other species of the genus . The peptidoglycan type of strain BS-12M was A4β based on -Orn − -Ser − -Glu. Strain BS-12M contained demethylmenaquinone-9(H) as the major menaquinone and anteiso-C and C as the major fatty acids. The major polar lipids of strain BS-12M were phosphatidylinositol and phosphatidylinositolmannoside. The DNA G+C content of strain BS-12M was 70.7 mol% and its DNA–DNA relatedness values with the type strains of five phylogenetically related species of the genus were 15–34 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain BS-12M is separate from other species of the genus . On the basis of the data presented, strain BS-12M is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BS-12M ( = KCTC 29674 = NBRC 110675).

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2015-07-01
2021-10-18
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