1887

Abstract

A Gram-staining-positive, rod-shaped, endospore-forming, aerobic bacterium (FJAT-17212) was isolated from the rhizosphere soil of a medical plant, (common selfheal), on the Wuyishan mountain of China. Isolate FJAT-17212 grew at 10–50 °C (optimum 30 °C), pH 5–11 (optimum pH 7) and with 0–6  % (w/v) NaCl (optimum 2  %). Phylogenetic analyses based on 16S rRNA gene sequences showed that isolate FJAT-17212 was a member of the genus and was most closely related to DSM 15595 (97.3  %). DNA–DNA relatedness between isolate FJAT-17212 and DSM 15595 was low (35.2  % ± 2.3). The diagnostic diamino acid of the peptidoglycan of isolate FJAT-17212 was -diaminopimelic acid and the predominant isoprenoid quinone was MK-7 (80.8  %). The major cellular fatty acids were iso-C (35.7  %), anteiso-C (29.8  %), iso-C (9.9  %) and iso-C (9.9  %) and the DNA G+C content was 39.8  mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate FJAT-17212 represents a novel species within the genus , for which the name Bacillus sp. nov. is proposed. The type strain is FJAT-17212 ( = DSM 27848 = CGMCC 1.12709).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000215
2015-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/7/2030.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000215&mimeType=html&fmt=ahah

References

  1. Ait Kaki A., Kacem Chaouche N., Dehimat L., Milet A., Youcef-Ali M., Ongena M., Thonart P. ( 2013;). Biocontrol and plant Growth promotion characterization of Bacillus Species isolated from Calendula officinalis rhizosphere. Indian J Microbiol 53 447452 [View Article] [PubMed].
    [Google Scholar]
  2. Albert R.A., Archambault J., Rosselló-Mora R., Tindall B.J., Matheny M. ( 2005;). Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55 21252130 [View Article] [PubMed].
    [Google Scholar]
  3. Atlas R.M. ( 1993). Handbook of Microbiological Media. Edited by Parks L. C. Boca Raton, FL: CRC Press;.
    [Google Scholar]
  4. Berkeley R.C.W. ( 2002;). Whither Bacillus?. In Applications and Systematics of Bacillus and Relatives, pp. 17 [View Article] Edited by Berkeley R., Heyndrickx M., Logan N., De Vos P. Oxford: Blackwell;.
    [Google Scholar]
  5. Chen Y.G., Cui X.L., Pukall R., Li H.M., Yang Y.L., Xu L.H., Wen M.L., Peng Q., Jiang C.L. ( 2007;). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57 23272332 [View Article] [PubMed].
    [Google Scholar]
  6. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221230 [View Article] [PubMed].
    [Google Scholar]
  7. Cowan S.T., Steel K.J. ( 1965). Manual for the Identification of Medical Bacteria., London: Cambridge University Press;.
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133142 [View Article] [PubMed].
    [Google Scholar]
  9. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  10. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  11. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  12. Gregersen T. ( 1978;). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5 123127 [View Article].
    [Google Scholar]
  13. Groth I., Schumann P., Weiss N., Martin K., Rainey F.A. ( 1996;). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46 234239 [View Article] [PubMed].
    [Google Scholar]
  14. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. ( 1985). Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: John Innes Foundation;.
    [Google Scholar]
  15. Huß V.A., Festl H., Schleifer K.H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184192 [View Article] [PubMed].
    [Google Scholar]
  16. Jukes T.H., Cantor C.R. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism vol. 3, pp. 21132 [View Article] Edited by Munro H. N. New York: Academic Press;.
    [Google Scholar]
  17. Kämpfer P. ( 1994;). Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. Syst Appl Microbiol 17 8698 [View Article].
    [Google Scholar]
  18. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  19. Lane D.J., Pace B., Olsen G.J., Stahl D.A., Sogin M.L., Pace N.R. ( 1985;). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82 69556959 [View Article] [PubMed].
    [Google Scholar]
  20. Lee S.W., Lee S.H., Balaraju K., Park K.S., Nam K.W., Park J.W., Park K. ( 2014;). Growth promotion and induced disease suppression of four vegetable crops by a selected plant growth-promoting rhizobacteria (PGPR) strain Bacillus subtilis 21-1 under two different soil conditions. Acta Physiol Plant 36 13531362 [View Article].
    [Google Scholar]
  21. Lim J.M., Jeon C.O., Lee J.C., Ju Y.J., Park D.J., Kim C.J. ( 2006;). Bacillus koreensis sp. nov., a spore-forming bacterium, isolated from the rhizosphere of willow roots in Korea. Int J Syst Evol Microbiol 56 5963 [View Article] [PubMed].
    [Google Scholar]
  22. Liu B., Liu G.H., Hu G.H., Chen M.C. ( 2014;). Bacillus mesonae sp. nov., isolated from the root of Mesona chinensis . Int J Syst Evol Microbiol 64 33463352 [View Article] [PubMed].
    [Google Scholar]
  23. Logan N.A., De Vos P. ( 2009;). Genus I. Bacillus . . In Bergey's Manual of Systematic Bacteriology , 2nd edn.., vol. 3, pp. 21128. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. New York: Springer;.
    [Google Scholar]
  24. Logan N.A., Berge O., Bishop A.H., Busse H.J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59 21142121 [View Article] [PubMed].
    [Google Scholar]
  25. Madhaiyan M., Poonguzhali S., Kwon S.W., Sa T.M. ( 2010;). Bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil. Int J Syst Evol Microbiol 60 24902495 [View Article] [PubMed].
    [Google Scholar]
  26. Madhaiyan M., Poonguzhali S., Lee J.S., Lee K.C., Hari K. ( 2011;). Bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil. Antonie van Leeuwenhoek 100 437444 [View Article] [PubMed].
    [Google Scholar]
  27. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109118 [View Article] [PubMed].
    [Google Scholar]
  28. Murray R.G.E., Doetsch R.N., Robinow C.F. ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 2141. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  29. Priest F.G., Goodfellow M., Todd C. ( 1988;). A numerical classification of the genus Bacillus . J Gen Microbiol 134 18471882 [PubMed].
    [Google Scholar]
  30. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  31. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC News 20 16.
    [Google Scholar]
  32. Schleifer K.H. ( 1985;). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18 123156 [View Article].
    [Google Scholar]
  33. Singh R.K., Kumar D.P., Singh P., Solanki M.K., Srivastava S., Kashyap P.L., Kumar S., Srivastava A.K., Singhal P.K., Arora D.K. ( 2014;). Multifarious plant growth promoting characteristics of chickpea rhizosphere associated Bacilli help to suppress soil-borne pathogens. Plant Growth Regul 73 91101 [View Article].
    [Google Scholar]
  34. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  35. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849 [View Article].
    [Google Scholar]
  36. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  37. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed].
    [Google Scholar]
  38. Wayne L.G. ( 1988;). International Committee on Systematic Bacteriology: announcement of the report of the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Zentralbl Bakteriol Mikrobiol Hyg [A] 268 433434 [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000215
Loading
/content/journal/ijsem/10.1099/ijs.0.000215
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error