1887

Abstract

A Gram-staining-positive, rod-shaped, endospore-forming, aerobic bacterium (FJAT-17212) was isolated from the rhizosphere soil of a medical plant, (common selfheal), on the Wuyishan mountain of China. Isolate FJAT-17212 grew at 10–50 °C (optimum 30 °C), pH 5–11 (optimum pH 7) and with 0–6  % (w/v) NaCl (optimum 2  %). Phylogenetic analyses based on 16S rRNA gene sequences showed that isolate FJAT-17212 was a member of the genus and was most closely related to DSM 15595 (97.3  %). DNA–DNA relatedness between isolate FJAT-17212 and DSM 15595 was low (35.2  % ± 2.3). The diagnostic diamino acid of the peptidoglycan of isolate FJAT-17212 was -diaminopimelic acid and the predominant isoprenoid quinone was MK-7 (80.8  %). The major cellular fatty acids were iso-C (35.7  %), anteiso-C (29.8  %), iso-C (9.9  %) and iso-C (9.9  %) and the DNA G+C content was 39.8  mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate FJAT-17212 represents a novel species within the genus , for which the name Bacillus sp. nov. is proposed. The type strain is FJAT-17212 ( = DSM 27848 = CGMCC 1.12709).

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2015-07-01
2019-10-22
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