1887

Abstract

A novel Gram-positive-staining, strictly aerobic and heterotrophic bacterium, designated strain LL-002, was isolated from organics- and methane-rich seafloor sediment at a depth of 100 m in Kagoshima Bay, Kagoshima, Japan. Colonies were lustreless and translucent white in colour. The temperature, pH and salt concentration ranges for growth were 10–30 °C, pH 6.0–6.5 and 0–1 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain LL-002 belongs to the genus of the family . 16S rRNA gene sequence similarities between strain LL-002 and the type strains of species of the genus were 92.8–95.7 %; the highest sequence identity was with the type strain of . The DNA G+C content of strain LL-002 was 46.2 mol%. MK-7 was the predominant menaquinone. The predominant cellular fatty acids were iso-C and anteiso-C, and the cell-wall peptidoglycan contained -diaminopimelic acid and glutamic acid, glycine and alanine in addition to muramic acid and glucosamine. The peptidoglycan type was A1γ. In DNA–DNA hybridization assays between strain LL-002 and the type strains of the other species of the genus , the level of hybridization was 6.3–30.1 %. On the basis of its biological features and the 16S rRNA gene sequence comparison presented here, strain LL-002 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is LL-002 ( = NBRC 110097 = CECT 8536).

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2015-06-01
2019-10-23
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