1887

Abstract

A facultatively anaerobic bacterium, designated strain 1MBB1, was isolated from basaltic breccia collected from 341 m below the seafloor by seafloor drilling of Rigil Guyot during Integrated Ocean Drilling Program Expedition 330. The cells were straight rods, 0.5 μm wide and 1–3 μm long, that occurred singly and in chains. Strain 1MBB1 stained Gram-positive. Catalase and oxidase were produced. The isolate grew optimally at 30 °C and pH 7.5, and could grow with up to 12 % (w/v) NaCl. The DNA G+C content was 40.5 mol%. The major cellular fatty acids were C ω11 (26.5 %), anteiso-C (19.5 %), C (18.7 %) and iso-C (10.4 %), and the cell-wall diamino acid was -diaminopimelic acid. Endospores of strain 1MBB1 oxidized Mn(II) to Mn(IV), and siderophore production by vegetative cells was positive. Phylogenetic analysis of the 16S rRNA gene indicated that strain 1MBB1 was a member of the family , with CV53 and LMG 21837 being the closest phylogenetic neighbours (96.5 and 96.2 % similarity, respectively). This is the first novel species described from deep subseafloor basaltic crust. On the basis of our polyphasic analysis, we conclude that strain 1MBB1 represents a novel species of the genus , for which we propose the name Bacillus sp. nov. The type strain is 1MBB1 ( = NCMA B78 = LMG 28275).

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2015-06-01
2019-10-21
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