1887

Abstract

Polyphasic taxonomic analysis was performed on a clinical isolate (NML 06-3099) from a cystic fibrosis patient, including whole-genome sequencing, proteomics, phenotypic testing, electron microscopy, chemotaxonomy and a clinical investigation. Comparative whole-genome sequence analysis and multilocus sequence analysis (MLSA) between ATCC 33301 and clinical isolate NML 06-3099 suggested that the clinical isolate was closely related to, but distinct from, the species . By 16S rRNA gene sequencing, the clinical isolate shared 98.7 % sequence identity with ATCC 33301. A concatenate of six MLSA loci (totalling 4500 bp) revealed < 93.9 % identity between ATCC 33301, other members of the genus and the clinical isolate. A whole-genome sequence comparison between NML 06-3099 and ATCC 33301 determined that the average nucleotide identity was 76.24 %. The overall DNA G+C content of NML 06-3099 was 51.27 %, consistent with members of the genus . By matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS analysis, NML 06-3099 had a genus-level match, but not a species-level match, to . By shotgun proteomics, ATCC 33301 and NML 06-3099 were found to have unique proteomes. The two strains had similar morphologies and multiple fimbriae, as observed by transmission electron microscopy, but were distinguishable by phenotypic testing. Cellular fatty acids found were typical for members of the . NML 06-3099 was susceptible to commonly used antibiotics. Based on these data, NML 06-3099 represents a novel species in the genus , for which the name sp. nov. is proposed (type strain NML 06-3099 = CCUG 55408 = DSM 19846).

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2015-06-01
2019-12-07
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