1887

Abstract

A heterotrophic, Gram-negative, aerobic bacterium, designated strain SB1, was isolated from surface water of the southern North Sea. Comparison of 16S rRNA gene sequences revealed that strain SB1 is affiliated to the genus within the marine clade (family ), with as the closest described species (98.2 % sequence similarity to the type strain). DNA–DNA hybridization indicated that SB1 represents a distinct species within this genus. On marine agar, strain SB1 formed beige, circular and convex colonies. Cells were irregular, motile rods. Growth occurred between 4 and 25 °C and was optimal at 20 °C, and at pH 7–9 (optimum pH 7.5–8.5) and NaCl concentrations between 1 and 6 % (optimum 2–4 %). The DNA G+C content of SB1 was 54.7 mol%. The fatty acids (>1 %) comprised 10 : 0 3-OH, 12 : 1 3-OH, 16 : 1ω7, 16 : 0, 18 : 2ω7,12, 18 : 1ω7, 18 : 0 and 11-methyl 18 : 1ω7. The sole respiratory lipoquinone was ubiquinone Q-10 and the polar lipid pattern indicated the presence of the phospholipids phosphatidylglycerol and phosphatidylcholine, as well as unidentified aminolipid AL1, phospholipids PL1 and PL3 and lipids L1, L2 and L4. On the basis of phylogenetic and phenotypic differences, strain SB1 represents a novel species in the genus , for which we propose the name sp. nov. The type strain is SB1 ( = DSM 26878 = LMG 27946). Furthermore, our results suggest the reclassification of as the type species of a new genus, gen. nov., as comb. nov. (type strain SSK2-1 = KCTC 32535 = CECT 8397). Finally, emended descriptions of the genus and its species and are also provided.

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2015-06-01
2020-01-21
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