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Volume 65, Issue Pt_6

sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil Free

Abstract

A Gram-stain-positive, endospore-forming, moderately halophilic bacterial strain, NEAU-ST10-40, was isolated from a saline and alkaline soil in Anda City, China. It was strictly aerobic, rod-shaped and motile by peritrichous flagella. It formed light yellow colonies and grew at NaCl concentrations of 3–15 % (w/v) (optimum, 8 %, w/v), at pH 7.0–9.0 (optimum, pH 8.0) and at 4–60 °C (optimum, 30 °C). It contained -diaminopimelic acid in the cell-wall peptidoglycan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genus . Levels of 16S rRNA gene sequence similarity between strain NEAU-ST10-40 and the type strains of related species of the genus ranged from 98.8 % ( FP5) to 97.1 % ( IS-Hb7). DNA–DNA hybridization relatedness values between strain NEAU-ST10-40 and DSM 18525, KCTC 13144, DSM 17110, DSM 2266, DSM 10405, DSM 18199, DSM 18897, DSM 21183, DSM 10404 and DSM 21185 were from 43 ± 1 to 19 ± 1 % (mean ± ). The DNA G+C content was 39.3 mol%. The major fatty acids (>10 %) were anteiso-C, anteiso-C and iso-C, the only respiratory quinone detected was MK-7, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and three unknown lipids. On the basis of the data presented, strain NEAU-ST10-40 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is NEAU-ST10-40 ( = CGMCC 1.12153 = DSM 25866).

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2015-06-01
2024-04-23
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References

  1. Amoozegar M.A., Malekzadeh F., Malik K.A., Schumann P., Spröer C. ( 2003;). Halobacillus karajensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 53 1059–1063. [CrossRef]
    [Google Scholar]
  2. An S.-Y., Kanoh K., Kasai H., Goto K., Yokota A. ( 2007;). Halobacillus faecis sp. nov., a spore-forming bacterium isolated from a mangrove area on Ishigaki Island, Japan. Int J Syst Evol Microbiol 57 2476–2479. [CrossRef]
    [Google Scholar]
  3. Chen Y.G., Liu Z.X., Zhang Y.Q., Zhang Y.X., Tang S.K., Borrathybay E., Li W.J., Cui X.L. ( 2009a;). Halobacillus naozhouensis sp. nov., a moderately halophilic bacterium isolated from a sea anemone. Antonie van Leeuwenhoek 96 99–107. [CrossRef]
    [Google Scholar]
  4. Chen Y.G., Zhang Y.Q., Liu Z.X., Zhuang D.C., Klenk H.P., Tang S.K., Cui X.L., Li W.J. ( 2009b;). Halobacillus salsuginis sp. nov., a moderately halophilic bacterium from a subterranean brine. Int J Syst Evol Microbiol 59 2505–2509. [CrossRef]
    [Google Scholar]
  5. Claus D., Fahmy F., Rolf H.J., Tosunoglu N. ( 1983;). Sporosarcina halophila sp. nov., an obligate, slightly halophilic bacterium from salt marsh soils. Syst Appl Microbiol 4 496–506. [CrossRef]
    [Google Scholar]
  6. Cowan S.T., Steel K.J. ( 1965). Manual for the Identification of Medical Bacteria., London: Cambridge University Press;.
     [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133–142. [CrossRef]
    [Google Scholar]
  8. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376. [CrossRef]
    [Google Scholar]
  9. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791. [CrossRef]
    [Google Scholar]
  10. Hasegawa T., Takizawa M., Tanida S. ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29 319–322. [CrossRef]
    [Google Scholar]
  11. Hua N.-P., Kanekiyo A., Fujikura K., Yasuda H., Naganuma T. ( 2007;). Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. Int J Syst Evol Microbiol 57 1243–1249. [CrossRef]
    [Google Scholar]
  12. Huss V.A.R., Festl H., Schleifer K.H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184–192. [CrossRef]
    [Google Scholar]
  13. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721. [CrossRef]
    [Google Scholar]
  14. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111–120. [CrossRef]
    [Google Scholar]
  15. Kluge A.G., Farris F.S. ( 1969;). Quantitative phyletics and the evolution of anurans. Syst Zool 18 1–32. [CrossRef]
    [Google Scholar]
  16. Kuykendall L.D., Roy M.A., O'Neill J.J., Devine T.E. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38 358–361. [CrossRef]
    [Google Scholar]
  17. Lee J.S., Shin Y.K., Yoon J.H., Takeuchi M., Pyun Y.R., Park Y.H. ( 2001;). Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov., and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. Int J Syst Evol Microbiol 51 1491–1498.
     [Google Scholar]
  18. Liu W.Y., Zeng J., Wang L., Dou Y.T., Yang S.S. ( 2005;). Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. Int J Syst Evol Microbiol 55 1991–1996. [CrossRef]
    [Google Scholar]
  19. Logan N.A., Berge O., Bishop A.H., Busse H.J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59 2114–2121. [CrossRef]
    [Google Scholar]
  20. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208–218. [CrossRef]
    [Google Scholar]
  21. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109–118. [CrossRef]
    [Google Scholar]
  22. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241. [CrossRef]
    [Google Scholar]
  23. Pan Y., Huang H., Meng J., Xiao H., Li C., Meng L., Hong S., Liu H., Wang X., Jiang J. ( 2012;). [Biodiversity of the culturable halotolerant and halophilic bacteria isolated from saline-alkaline soils in Songnen Plain]. Acta Microbiol Sin 52 1187–1194.
     [Google Scholar]
  24. Parte A.C. ( 2014;). LPSN–list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42 (D1), D613–D616. [CrossRef]
    [Google Scholar]
  25. Romano I., Finore I., Nicolaus G., Huertas F.J., Lama L., Nicolaus B., Poli A. ( 2008;). Halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in Fuente de Piedra, southern Spain. Int J Syst Evol Microbio 58 886–890. [CrossRef]
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425.
     [Google Scholar]
  27. Sehgal S.N., Gibbons N.E. ( 1960;). Effect of some metal ions on the growth of Halobacterium cutirubrum . Can J Microbiol 6 165–169. [CrossRef]
    [Google Scholar]
  28. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  29. Soto-Ramírez N., Sánchez-Porro C., Rosas-Padilla S., Almodóvar K., Jiménez G., Machado-Rodríguez M., Zapata M., Ventosa A., Montalvo-Rodríguez R. ( 2008;). Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans . Int J Syst Evol Microbiol 58 125–130. [CrossRef]
    [Google Scholar]
  30. Spring S., Ludwig W., Marquez M.C., Ventosa A., Schleifer K.-H. ( 1996;). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46 492–496. [CrossRef]
    [Google Scholar]
  31. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739. [CrossRef]
    [Google Scholar]
  32. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882. [CrossRef]
    [Google Scholar]
  33. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463–464. [CrossRef]
    [Google Scholar]
  34. Xu X.W., Wu Y.H., Zhou Z., Wang C.S., Zhou Y.G., Zhang H.B., Wang Y., Wu M. ( 2007;). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57 1619–1624. [CrossRef]
    [Google Scholar]
  35. Yoon J.-H., Kang K.H., Park Y.-H. ( 2003;). Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53 687–693. [CrossRef]
    [Google Scholar]
  36. Yoon J.-H., Kang K.H., Oh T.-K., Park Y.-H. ( 2004;). Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 8 23–28. [CrossRef]
    [Google Scholar]
  37. Yoon J.-H., Kang S.-J., Lee C.-H., Oh H.W., Oh T.-K. ( 2005;). Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 55 2413–2417. [CrossRef]
    [Google Scholar]
  38. Yoon J.-H., Kang S.-J., Jung Y.-T., Oh T.-K. ( 2007;). Halobacillus campisalis sp. nov., containing meso-diaminopimelic acid in the cell-wall peptidoglycan, and emended description of the genus Halobacillus . Int J Syst Evol Microbiol 57 2021–2025. [CrossRef]
    [Google Scholar]
  39. Yoon J.-H., Kang S.-J., Oh T.-K. ( 2008;). Halobacillus seohaensis sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 58 622–627. [CrossRef]
    [Google Scholar]
  40. Zhou Y., Dong J., Wang X., Huang X., Zhang K.Y., Zhang Y.Q., Guo Y.F., Lai R., Li W.J. ( 2007;). Chryseobacterium flavum sp. nov., isolated from polluted soil. Int J Syst Evol Microbiol 57 1765–1769. [CrossRef]
    [Google Scholar]
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Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil
Int J Syst Evol Microbiol 65, 1908 (2015); https://doi.org/10.1099/ijs.0.000198
/content/journal/ijsem/10.1099/ijs.0.000198
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