1887

Abstract

A Gram-stain-positive, endospore-forming, moderately halophilic bacterial strain, NEAU-ST10-40, was isolated from a saline and alkaline soil in Anda City, China. It was strictly aerobic, rod-shaped and motile by peritrichous flagella. It formed light yellow colonies and grew at NaCl concentrations of 3–15 % (w/v) (optimum, 8 %, w/v), at pH 7.0–9.0 (optimum, pH 8.0) and at 4–60 °C (optimum, 30 °C). It contained -diaminopimelic acid in the cell-wall peptidoglycan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genus . Levels of 16S rRNA gene sequence similarity between strain NEAU-ST10-40 and the type strains of related species of the genus ranged from 98.8 % ( FP5) to 97.1 % ( IS-Hb7). DNA–DNA hybridization relatedness values between strain NEAU-ST10-40 and DSM 18525, KCTC 13144, DSM 17110, DSM 2266, DSM 10405, DSM 18199, DSM 18897, DSM 21183, DSM 10404 and DSM 21185 were from 43 ± 1 to 19 ± 1 % (mean ± ). The DNA G+C content was 39.3 mol%. The major fatty acids (>10 %) were anteiso-C, anteiso-C and iso-C, the only respiratory quinone detected was MK-7, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and three unknown lipids. On the basis of the data presented, strain NEAU-ST10-40 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is NEAU-ST10-40 ( = CGMCC 1.12153 = DSM 25866).

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2015-06-01
2021-10-15
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