1887

Abstract

Three isolates originating from Spanish-style green-olive fermentations in a manufacturing company in the province of Seville, Spain, were taxonomically characterized by a polyphasic approach. This included a phylogenetic analysis based on 16S rRNA gene sequences and multi-locus sequence analysis (MLSA) based on , , , and genes. The isolates shared 98.0 % 16S rRNA gene sequence similarity with G21. Phylogenetic analysis based on 16S rRNA gene sequences using the neighbour-joining and maximum-likelihood methods showed that the isolates fell within the genus and formed an independent branch close to G21. The maximum-parsimony method grouped the isolates to G21 but forming two clearly separated branches. Phylogenetic trees based on individual , , , and gene sequences revealed that strain IGJ1.11 formed a clade alone or with G21. Sequence similarities of the , , , and genes between strain IGJ1.11 and G21 were 86.7, 85.7, 97.3, 87.6 and 84.8 %, respectively. MLSA of concatenated sequences showed that strain IGJ1.11 and G21 are two clearly separated species that form a clade, which we named Clade , that presented 89.7 % concatenated gene sequence similarity, i.e. less than 92 %. The major cellular fatty acids (>5 %) of strain IGJ1.11 were summed feature 3 (Cω7 and/or Cω6), C and summed feature 8 (Cω7 and/or Cω6). Enzymic activity profiles, sugar fermentation patterns and DNA G+C content (52.9 mol%) differentiated the novel strains from the closest related members of the genus . The name sp. nov. is proposed for the novel species. The type strain is IGJ1.11 ( = CECT 8064 = DSM 25438).

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2015-06-01
2019-10-23
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