1887

Abstract

A Gram-staining-negative, strictly heterotrophic and aerobic bacterium, strain TS-T44, was isolated from a saline lake, Tuosu Lake in Qaidam basin, Qinghai province, China. Its taxonomic position was investigated using a polyphasic approach. Cells of strain TS-T44 were non-endospore-forming, non-motile rods, 0.8–1.2 μm wide and 1.2–3.0 μm long. Catalase- and oxidase-positive. Growth occurred in the presence of up to 8  % (w/v) NaCl (optimum, 3.0  %) and at 15–35 °C (optimum, 25 °C) and pH 7.0–10.0 (optimum, pH 7.5–8.5). Cω7 was the predominant fatty acid. The major respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and an unknown lipid. The DNA G+C content was 65.5 mol% [determined from the melting temperature (m)]. Phylogenetic trees based on 16S rRNA gene sequences showed that strain TS-T44 was associated with the genus and showed highest sequence similarity to CL-SK44 (97.7  %), CL-JM1 (97.5  %) and DPG-138 (97.3  %), and < 97  % to other species. DNA–DNA relatedness of strain TS-T44 to JCM 15447, JCM 15446 and KCTC 23882 was 23 ± 3  %, 33 ± 4  % and 35 ± 2  %, respectively. Based on the data presented, it is concluded that strain TS-T44 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TS-T44 ( = CGMCC 1.12478 = JCM 19516).

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2015-06-01
2024-03-28
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References

  1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. ( 1990;). Basic local alignment search tool. J Mol Biol 215 403410 [View Article] [PubMed].
    [Google Scholar]
  2. Collins M.D. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. London: Academic Press;.
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133142 [View Article] [PubMed].
    [Google Scholar]
  4. Dong X.Z., Cai M.Y. ( 2001). Determinative Manual for Routine Bacteriology., Beijing: Scientific Press; (English translation) .
    [Google Scholar]
  5. Eguchi M., Nishikawa T., Macdonald K., Cavicchioli R., Gottschal J.C., Kjelleberg S. ( 1996;). Responses to stress and nutrient availability by the marine ultramicrobacterium Sphingomonas sp. strain RB2256. Appl Environ Microbiol 62 12871294 [PubMed].
    [Google Scholar]
  6. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  7. Fitch W.M. ( 1971;). Toward defining course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  8. Huß V.A., Festl H., Schleifer K.H. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4 184192 [View Article] [PubMed].
    [Google Scholar]
  9. Hwang C.Y., Bae G.D., Yih W., Cho B.C. ( 2009;). Marivita cryptomonadis gen. nov, sp. nov. and Marivita litorea sp. nov., of the family Rhodobacteraceae, isolated from marine habitats. Int J Syst Evol Microbiol 59 15681575 [View Article] [PubMed].
    [Google Scholar]
  10. Kates M. ( 1986). Techniques of Lipidology , 2nd edn.., Amsterdam: Elsevier;.
    [Google Scholar]
  11. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  12. Kimura M. doi:10.1017/CBO9780511623486 ( 1983). The Neutral Theory of Molecular Evolution., Cambridge: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  13. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109118 [View Article] [PubMed].
    [Google Scholar]
  14. Nokhal T.H., Schlegel H.G. ( 1983;). Taxonomic study of Paracoccus denitrificans . Int J Syst Bacteriol 33 2637 [View Article].
    [Google Scholar]
  15. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  17. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed].
    [Google Scholar]
  18. Vandamme P., Pot B., Gillis M., de Vos P., Kersters K., Swings J. ( 1996;). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60 407438 [PubMed].
    [Google Scholar]
  19. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697703 [PubMed].
    [Google Scholar]
  20. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing) 16 176178.
    [Google Scholar]
  21. Yoon J.H., Kang S.J., Jung Y.T., Oh T.K. ( 2010;). Gaetbulicola byunsanensis gen. nov., sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 60 196199 [View Article] [PubMed].
    [Google Scholar]
  22. Yoon J.H., Kang S.J., Lee S.Y., Jung Y.T., Lee J.S., Oh T.K. ( 2012;). Marivita hallyeonensis sp. nov., isolated from seawater, reclassification of Gaetbulicola byunsanensis as Marivita byunsanensis comb. nov. and emended description of the genus Marivita Hwang et al. 2009. Int J Syst Evol Microbiol 62 839843 [View Article] [PubMed].
    [Google Scholar]
  23. Yoon J.H., Kang S.J., Lee J.S. ( 2013;). Marivita geojedonensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63 423427 [View Article] [PubMed].
    [Google Scholar]
  24. Zhong Z.P., Liu Y., Liu H.C., Wang F., Zhou Y.G., Liu Z.P. ( 2014;). Roseibium aquae sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 64 28122818 [View Article] [PubMed].
    [Google Scholar]
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