1887

Abstract

YF-3 is a Gram-stain-negative, non-motile, non-spore-forming, yellow–orange, rod-shaped bacterium. Optimal growth conditions were at 30 °C, pH 7.0 and 1 % (w/v) NaCl. Phylogenetic analysis, on the basis of the 16S rRNA gene sequence, showed that strain YF-3 was closely related to the strains AG13 and Soil-3-27 with 98.71 % and 96.93 % sequence similarity, respectively. Strain YF-3 contained MK-6 as the main menaquinone and had a polyamine pattern with -homospermidine as the major component. Its major polar lipid was phosphatidylethanolamine. The dominant fatty acids of strain YF-3 were iso-C, iso-C 3-OH, summed feature 9 (comprising iso-Cω9 and/or C 10-methyl) and summed feature 3 (comprising Cω7 and/or Cω6). The DNA G+C content of strain YF-3 was 37 mol%. The DNA–DNA relatedness levels between strain YF-3 and the most closely related strains, AG13 and Soil-3-27, were 31.7 ± 2.1 % and 28.4 ± 5.4 %, respectively. Based on these results, a novel species named sp. nov. is proposed. The type strain is YF-3 ( = CCTCC AB 2014060 = JCM 30154).

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2015-06-01
2020-11-24
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