sp. nov. and sp. nov., isolated from soil and emended description of the genus Marmoricola Free

Abstract

Two strains of species of the genus , designated KIS18-7 and JOS5-1, were isolated from soil samples in Korea. The 16S rRNA gene sequence of strain KIS18-7 showed highest similarities with Sco-D01 (97.8 %), SST-45 (97.6 %) and BC 361 (97.3 %), and strain JOS5-1 had highest sequence similarities with SST-45 (97.5 %) and MSL-05 (97.3 %). The sequence similarity between KIS18-7 and JOS5-1 was 98.1 %. Phylogenetic analysis showed that these strains grouped with species of the genus . The major fatty acids of strain KIS18-7 were iso-C and Cω8, and Cω8, C 10-methyl (TBSA), Cω9, C 10-methyl and C 2-OH for strain JOS5-1. Strain KIS18-7 contained the polar lipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine, while strain JOS5-1 contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown aminolipid and two unknown phospholipids. The peptidoglycan of both strains contained -diaminopimelic acid as the diagnostic diamino acid and a single glycine residue as the interpeptide bridge (type A3γ). The major menaquinone of both strains was MK-8(H). The G+C contents of the DNA of strains KIS18-7 and JOS5-1 were 68.0 mol% and 62.9 mol%, respectively. These data demonstrate that strains KIS18-7 and JOS5-1 are representatives of two novel species of the genus , for which the names sp. nov. (type strain KIS18-7 = KACC 17307 = DSM 27140 = NBRC 109601) and sp. nov. (type strain JOS5-1 = KACC 17308 = DSM 27141 = NBRC 109602) are proposed.

Funding
This study was supported by the:
  • National Academy of Agricultural Science, Rural Development Administration, Republic of Korea (Award PJ011248)
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2015-06-01
2024-03-28
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References

  1. Breznak J. A., Costilow R. N. ( 1994 ). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  2. Dastager S. G., Lee J. C., Ju Y. J., Park D. J., Kim C. J. ( 2008 ). Marmoricola bigeumensis sp. nov., a member of the family Nocardioidaceae . . Int J Syst Evol Microbiol 58, 10601063. [View Article] [PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  4. Felske A., Rheims H., Wolterink A., Stackebrandt E., Akkermans A. D. L. ( 1997 ). Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils. . Microbiology 143, 29832989. [View Article] [PubMed]
    [Google Scholar]
  5. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  6. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770773. [View Article] [PubMed]
    [Google Scholar]
  7. Hamada M., Tamura T., Yamamura H., Suzuki K., Hayakawa M. ( 2012 ). Lysinimicrobium mangrovi gen. nov., sp. nov., an actinobacterium isolated from the rhizosphere of a mangrove. . Int J Syst Evol Microbiol 62, 17311735. [View Article] [PubMed]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  9. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  10. Lee S. D. ( 2007 ). Marmoricola aequoreus sp. nov., a novel actinobacterium isolated from marine sediment. . Int J Syst Evol Microbiol 57, 13911395. [View Article] [PubMed]
    [Google Scholar]
  11. Lee D. W., Lee S. D. ( 2010 ). Marmoricola scoriae sp. nov., isolated from volcanic ash. . Int J Syst Evol Microbiol 60, 21352139. [View Article] [PubMed]
    [Google Scholar]
  12. Lee S. D., Lee D. W., Ko Y. H. ( 2011 ). Marmoricola korecus sp. nov.. Int J Syst Evol Microbiol 61, 16281631. [View Article] [PubMed]
    [Google Scholar]
  13. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  14. Pruesse E., Peplies J., Glöckner F. O. ( 2012 ). SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28, 18231829. [View Article] [PubMed]
    [Google Scholar]
  15. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  16. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  17. Seldin L., Dubnau D. ( 1985 ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. . Int J Syst Bacteriol 35, 151154. [View Article]
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  19. Urzì C., Salamone P., Schumann P., Stackebrandt E. ( 2000 ). Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. . Int J Syst Evol Microbiol 50, 529536. [View Article] [PubMed]
    [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
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