1887

Abstract

A novel actinomycete, designated strain NEAU-FHS4, was isolated from the root of black false hellebore ( L.). Strain NEAU-FHS4 formed single spores with smooth surfaces on substrate mycelium. The novel strain contained -diaminopimelic as amino acid of the peptidoglycan and xylose and glucose as whole-cell sugars. The predominant menaquinones were MK-10(H) and MK-10(H). Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were iso-C, C, C and anteiso-C. Phenotypic and chemotaxonomic analysis showed that the novel isolate had characteristics typical of members of the genus . 16S rRNA gene sequence analysis also indicated that strain NEAU-FHS4 belonged to the genus , with highest sequence similarities to YIM 61359 (98.88 %) and YIM 68255 (98.85 %). The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the novel strain from the most closely related strains. Based on morphological, chemotaxonomic and phylogenetic data, strain NEAU-FHS4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-FHS4 ( = CGMCC 4.7143 = DSM 46718).

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2015-06-01
2020-01-18
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