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Volume 65, Issue Pt_6

sp. nov., an actinomycete isolated from black false hellebore root ( L.) Free

Abstract

A novel actinomycete, designated strain NEAU-FHS4, was isolated from the root of black false hellebore ( L.). Strain NEAU-FHS4 formed single spores with smooth surfaces on substrate mycelium. The novel strain contained -diaminopimelic as amino acid of the peptidoglycan and xylose and glucose as whole-cell sugars. The predominant menaquinones were MK-10(H) and MK-10(H). Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were iso-C, C, C and anteiso-C. Phenotypic and chemotaxonomic analysis showed that the novel isolate had characteristics typical of members of the genus . 16S rRNA gene sequence analysis also indicated that strain NEAU-FHS4 belonged to the genus , with highest sequence similarities to YIM 61359 (98.88 %) and YIM 68255 (98.85 %). The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the novel strain from the most closely related strains. Based on morphological, chemotaxonomic and phylogenetic data, strain NEAU-FHS4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-FHS4 ( = CGMCC 4.7143 = DSM 46718).

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Funding
This study was supported by the:
  • National Outstanding Youth Foundation (Award 31225024)
  • National Key Project for Basic Research (Award 2010CB126102)
  • National Key Technology R&D Program (Award 2012BAD19B06)
  • National Natural Science Foundation of China (Award 31471832, 31071750, 31171913 and 31372006)
  • Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP)
  • Heilongjiang Provincial Educational Commission (Award 12541001)
  • Youth Science Foundation of Heilongjiang Province (Award QC2014C013)
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2015-06-01
2024-04-19
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Plantactinospora veratri sp. nov., an actinomycete isolated from black false hellebore root (Veratrum nigrum L.)
Int J Syst Evol Microbiol 65, 1799 (2015); https://doi.org/10.1099/ijs.0.000180
/content/journal/ijsem/10.1099/ijs.0.000180
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