1887

Abstract

Psychrophilic bacterial strains were isolated from alpine glaciers in Switzerland and characterized taxonomically. On the basis of phylogenetic analysis of partial 16S rRNA and genes, three of those strains, strain 79 ( = CCOS 247), strain 4/58 ( = CCOS 250) and strain 4/56 ( = CCOS 258) clustered together with strain Cr9-12 and separately from the type strains Cr9-30 and LMG 27070. Strain Cr9-12 has been previously described as a strain of . The three newly isolated strains were compared phenotypically with strain Cr9-12 and with the type strains of the species and . Cr9-12 and the three novel strains from an alpine glacier in Switzerland were Gram-stain-negative, non-motile, rod-shaped and psychrophilic and showed good growth throughout a temperature range of 1–20 °C and characteristically oxidized -mannitol, -fucose and bromosuccinic acid. The predominant cellular fatty acids of strain Cr9-12 and the three novel strains were summed feature 3 (Cω7 and/or iso-C 2-OH), C and Cω7. The respiratory quinone of these strains was ubiquinone 8 (UQ-8). The genomic DNA G+C content of Cr9-12 was 49.2 mol%. The combined data from phenotypic, phylogenetic and DNA–DNA relatedness studies strongly support the reclassification of strain Cr9-12 as representing a novel species. This strain and the isolates 79 ( = CCOS 247), 4/58 ( = CCOS 250) and 4/56 ( = CCOS 258) are representatives of a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is Cr9-12 ( = CCOS 761 = DSM 22814).

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2015-06-01
2019-10-21
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