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Abstract

A novel actinomycete, A-T 8343 was isolated from a moist evergreen forest soil sample collected in the Trat Province, Thailand. Based on 16S rRNA gene sequence analysis, strain A-T 8343 belonged to the genus and was closely related to A-T 1946 (98.81 %) and 6014 (98.54 %). The DNA–DNA relatedness values were 21.8–27 % with A-T 1946 and 31.1–31.9 % with 6014, which were significantly below 70 %. The result differentiated A-T 8343 from the closest species. The organism developed spherical sporangia containing non-motile spores on aerial mycelia. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinones were MK-9(H) and MK-9(H). The diagnostic phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, diphosphatidylglycerol, lyso-phosphatidylethanolamine, -acetylglucosamine-containing phospholipids, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannosides, aminophosphoglycolipid and one unknown phospholipid. The major cellular fatty acids were saturated C, iso C, unsaturated C and C. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the new isolate is proposed as a representative novel species of the genus to be named sp. nov. The type strain is A-T 8343 ( = BCC 52770 = NBRC 109516).

Funding
This study was supported by the:
  • , Bioresources Research Network (BRN) of National Center for Genetic Engineering and Biotechnology
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2015-06-01
2020-09-27
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