1887

Abstract

A novel actinomycete, A-T 8343 was isolated from a moist evergreen forest soil sample collected in the Trat Province, Thailand. Based on 16S rRNA gene sequence analysis, strain A-T 8343 belonged to the genus and was closely related to A-T 1946 (98.81 %) and 6014 (98.54 %). The DNA–DNA relatedness values were 21.8–27 % with A-T 1946 and 31.1–31.9 % with 6014, which were significantly below 70 %. The result differentiated A-T 8343 from the closest species. The organism developed spherical sporangia containing non-motile spores on aerial mycelia. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinones were MK-9(H) and MK-9(H). The diagnostic phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, diphosphatidylglycerol, lyso-phosphatidylethanolamine, -acetylglucosamine-containing phospholipids, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannosides, aminophosphoglycolipid and one unknown phospholipid. The major cellular fatty acids were saturated C, iso C, unsaturated C and C. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the new isolate is proposed as a representative novel species of the genus to be named sp. nov. The type strain is A-T 8343 ( = BCC 52770 = NBRC 109516).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000172
2015-06-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/6/1764.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000172&mimeType=html&fmt=ahah

References

  1. Arai T. . ( 1975; ). Culture Media for Actinomycetes. Tokyo:: The Society for Actinomycetes Japan;.
    [Google Scholar]
  2. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  3. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  4. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. . ( 1983; ). Parsimony in systematics: biological and statistical issues. . Annu Rev Ecol Syst 14:, 313–333. [CrossRef]
    [Google Scholar]
  6. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  8. Greenwood J. R. , Pickett M. J. . ( 1979; ). Salient features of Haemophilus vaginalis . . J Clin Microbiol 9:, 200–204.[PubMed]
    [Google Scholar]
  9. Itoh T. , Kudo T. , Parenti F. , Seino A. . ( 1989; ). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. . Int J Syst Bacteriol 39:, 168–173. [CrossRef]
    [Google Scholar]
  10. Kämpfer P. , Kroppenstedt R. M. . ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  11. Katsura K. , Kawasaki H. , Potacharoen W. , Saono S. , Seki T. , Yamada Y. , Uchimura T. , Komagata K. . ( 2001; ). Asaia siamensis sp. nov., an acetic acid bacterium in the α-proteobacteria . . Int J Syst Evol Microbiol 51:, 559–563.[PubMed]
    [Google Scholar]
  12. Kawasaki H. , Hoshino Y. , Hirata A. , Yamasato K. . ( 1993; ). Is intracytoplasmic membrane structure a generic criterion? It does not coincide with phylogenetic interrelationships among phototrophic purple nonsulfur bacteria. . Arch Microbiol 160:, 358–362. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. et al. ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721.[PubMed] [CrossRef]
    [Google Scholar]
  14. Komagata K. , Suzuki K. I. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  15. Lechevalier M. P. , Lechevalier H. . ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  16. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  17. Saito H. , Miura K. I. . ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72:, 619–629. [CrossRef] [PubMed]
    [Google Scholar]
  18. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI, Inc.
  20. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  21. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  22. Suriyachadkun C. , Chunhametha S. , Thawai C. , Tamura T. , Potacharoen W. , Kirtikara K. , Sanglier J. J. . ( 2009; ). Planotetraspora thailandica sp. nov., isolated from soil in Thailand. . Int J Syst Evol Microbiol 59:, 992–997. [CrossRef] [PubMed]
    [Google Scholar]
  23. Suriyachadkun C. , Ngaemthao W. , Chunhametha S. , Thawai C. , Sanglier J. J. , Kitpreechavanich V. . ( 2014; ). Sinosporangium siamense sp. nov., isolated from soil and emended description of the genus Sinosporangium . . Int J Syst Evol Microbiol 64:, 2828–2833. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  25. Tamura T. , Nakagaito Y. , Nishii T. , Hasegawa T. , Stackebrandt E. , Yokota A. . ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov.. Int J Syst Bacteriol 44:, 193–203. [CrossRef] [PubMed]
    [Google Scholar]
  26. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  27. Uchida K. , Aida K. . ( 1984; ). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. . J Gen Appl Microbiol 30:, 131–134. [CrossRef]
    [Google Scholar]
  28. Verlander C. P. . ( 1992; ). Detection of horseradish peroxidase by colorimetry. . In Nonisotopic DNA Probe Techniques, pp. 185–201. Edited by Kricka L. J. . . New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  29. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  30. Williams S. T. , Cross T. . ( 1971; ). Chapter XI Actinomycetes. . Methods Microbiol 4:, 295–334. [CrossRef]
    [Google Scholar]
  31. Wu C. , Lu X. , Qin M. , Wang Y. , Ruan J. . ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178.
    [Google Scholar]
  32. Yamada Y. , Katsura K. , Kawasaki H. , Widyastuti Y. , Saono S. , Seki T. , Uchimura T. , Komagata K. . ( 2000; ). Asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the α-Proteobacteria. . Int J Syst Evol Microbiol 50:, 823–829. [CrossRef] [PubMed]
    [Google Scholar]
  33. Zhang Y. Q. , Liu H. Y. , Yu L. Y. , Lee J. C. , Park D. J. , Kim C. J. , Xu L. H. , Jiang C. L. , Li W. J. . ( 2011; ). Sinosporangium album gen. nov., sp. nov., a new member of the suborder Streptosporangineae. . Int J Syst Evol Microbiol 61:, 592–597. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000172
Loading
/content/journal/ijsem/10.1099/ijs.0.000172
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error