1887

Abstract

A Gram-stain-positive, solvent-tolerating, aerobic, rod-shaped bacterium that formed terminal endospores was isolated from the organic solvent acetophenone. The strain, designated JC23, was oxidase- and catalase-positive. The strain grew in the presence of a wide range of organic solvents with partition coefficients (log p values) between 1 and 4, which are exceptionally toxic to micro-organisms. Based on 16S rRNA gene sequence analysis, strain JC23 was identified as belonging to the genus and was most closely related to Mn1-7 (98.5 % similarity), 440831 (97.2 %) and 2RL3-2 (96.8 %). DNA–DNA relatedness of strain JC23 with the type strains of the closest species was <39 %. Strain JC23 grew chemo-organoheterotrophically with optimal growth at pH 7 (range pH 6–9) and at 35 °C (range 25–40 °C). The DNA G+C content was 41 mol%. Major cellular fatty acids of strain JC23 were iso-C, iso-C, anteiso-C and anteiso-C. The cell-wall peptidoglycan type was determined to be A4α (-Lys–-Asp), which is in agreement with the cell-wall characteristics of the genus . The predominant quinone system was MK-7. Polar lipids of strain JC23 included diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids, β-gentiobiosyldiacylglycerol, two unidentified phospholipids and two unidentified lipids. On the basis of our morphological, physiological, genetic, phylogenetic and chemotaxonomic analyses, we conclude that strain JC23 should be assigned to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain JC23 ( = CCUG 57911 = KCTC 13605 = NBRC 105754 = DSM 23394).

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2015-06-01
2019-10-24
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