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Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, flexirubin-negative, rod-shaped, non-motile and psychrophilic bacterial strain, PAMC 27237, was isolated from marine sediment of the Ross Sea, Antarctica. Strain PAMC 27237 grew at 0–20 °C (optimally at 17 °C), at pH 5.0–9.5 (optimally at pH 7.0) and in the presence of 0–3.5 % (w/v) NaCl (optimally at 1.5–2.5 %). The major fatty acids (≥5 %) were iso-C 3-OH, C 2-OH, anteiso-C, summed feature 3 (Cω6/Cω7c), iso-C 3-OH, anteiso-Cω9, anteiso-C A, iso-C 3-OH and iso-C G. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids, four unidentified lipids and a glycolipid. The major respiratory quinone was MK-6. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain PAMC 27237 belongs to the genus showing high similarities with the type strains of (97.2 %), (97.0 %) and (96.4 %). Average nucleotide identity values between strain PAMC 27237 and the type strains of and were 83.1 and 84.2 %, respectively, and mean genome-to-genome distances were 22.4–24.2 %, indicating that strain PAMC 27237 is clearly distinguished from the most closely related species of the genus . The genomic DNA G+C content calculated from genome sequences was 33.5 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain PAMC 27237 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PAMC 27237 ( = KCTC 42130 = JCM 30370).

Funding
This study was supported by the:
  • Korea Polar Research Institute (Award PE14080)
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2015-06-01
2021-10-16
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