1887

Abstract

A moderately thermophilic, aerobic, stalked bacterium (strain MA2) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain MA2 was most closely related to the genera , and (92–93 % similarity) within the class . Strain MA2 was a Gram-stain-negative and stalked dimorphic bacteria. The temperature range for growth was 16–48 °C (optimum growth at 42 °C). This strain required yeast extract and NaCl (>1 %, w/v) for growth, tolerated up to 11 % (w/v) NaCl, and was capable of utilizing various carbon sources. The major cellular fatty acid and major respiratory quinone were Cω7 and ubiquinone-10, respectively. The DNA G+C content was 60.7 mol%. Strain MA2 performed denitrification and produced NO from nitrate under strictly microaerobic conditions. Strain MA2 possessed periplasmic nitrate reductase (Nap) genes but not membrane-bound nitrate reductase (Nar) genes. On the basis of this morphological, physiological, biochemical and genetic information a novel genus and species, gen. nov., sp. nov., are proposed, with MA2 ( = NBRC 109643 = DSM 27167) as the type strain of the species.

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2015-06-01
2019-10-20
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