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Volume 65, Issue Pt_6

gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions Free

Abstract

A moderately thermophilic, aerobic, stalked bacterium (strain MA2) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain MA2 was most closely related to the genera , and (92–93 % similarity) within the class . Strain MA2 was a Gram-stain-negative and stalked dimorphic bacteria. The temperature range for growth was 16–48 °C (optimum growth at 42 °C). This strain required yeast extract and NaCl (>1 %, w/v) for growth, tolerated up to 11 % (w/v) NaCl, and was capable of utilizing various carbon sources. The major cellular fatty acid and major respiratory quinone were Cω7 and ubiquinone-10, respectively. The DNA G+C content was 60.7 mol%. Strain MA2 performed denitrification and produced NO from nitrate under strictly microaerobic conditions. Strain MA2 possessed periplasmic nitrate reductase (Nap) genes but not membrane-bound nitrate reductase (Nar) genes. On the basis of this morphological, physiological, biochemical and genetic information a novel genus and species, gen. nov., sp. nov., are proposed, with MA2 ( = NBRC 109643 = DSM 27167) as the type strain of the species.

  • Published Online:
Funding
This study was supported by the:
  • Ministry of Education, Culture, Sports, Science and Technology of Japan (Award 221S0002 and 225450265)
  • The University of Tokyo (Award 005, 2013)
© 2015 IUMS
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2015-06-01
2024-04-19
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Tepidicaulis marinus gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions
Int J Syst Evol Microbiol 65, 1749 (2015); https://doi.org/10.1099/ijs.0.000167
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