RT Journal Article SR Electronic(1) A1 Xiong, Zi-Jun A1 Miao, Cui-Ping A1 Zheng, You-Kun A1 Liu, Kai A1 Li, Wen-Jun A1 Liu, Wei-Hong A1 Xu, Li-Hua A1 Zhao, Li-XingYR 2015 T1 Stackebrandtia endophytica sp. nov., an actinobacterium isolated from Tripterygium wilfordii JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_6 SP 1709 OP 1713 DO https://doi.org/10.1099/ijs.0.000166 PB Microbiology Society, SN 1466-5034, AB A novel endophytic actinobacterium, designated strain YIM 64602T, was isolated from healthy stems of Tripterygium wilfordii. It grew at 15–40 °C, pH 6.0–9.0 and in the presence of 0–3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YIM 64602T belongs to the genus Stackebrandtia . Whole-cell hydrolysates of strain YIM 64602T contained the amino acid meso-diaminopimelic acid with the sugars mannose, rhamnose and glucose, and a trace of ribose. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine and phosphatidylethanolamine. MK-10(H6), MK-10(H4) and MK-11(H4) were the predominant components in the quinone system. The fatty-acid pattern was mainly composed of the saturated branched-chain acids iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0. The DNA G+C content was 72.4 mol%. 16S rRNA gene sequence analysis showed the highest pairwise sequence identity (96.0–98.5 %) with the members of the genus Stackebrandtia . Strain YIM 64602T displayed a DNA–DNA relatedness of 43.9±0.4 % with the type strain Stackebrandtia albiflava YIM 45751T. Based on evidence from this polyphasic study, strain YIM 64602T ( = BCRC 16954T = DSM 45928T) is considered to represent a novel species of the genus Stackebrandtia , for which the name Stackebrandtia endophytica is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000166