1887

Abstract

A Gram-stain-negative, microaerophilic strain, 80(YS1), with a spiral-shaped morphology and 1–2 sheathed flagella at each end of the cells was isolated from the gastric mucosa of , the animal reservoir of in China, on the Qinghai-Tibet Plateau. The strain grew at 30, 35 and 42 °C, but not at 25 °C. Growth was in the form of a thinly spreading film on brain heart infusion agar containing 8 % sheep blood under microaerobic conditions. The strain did not hydrolyse urea or hippurate, and did not grow on media containing 1 % glycine. It reduced nitrate to nitrite, and was catalase- and alkaline-phosphatase-positive, susceptible to nalidixic acid and resistant to cefalotin. It was positive for genus-specific PCR for the genus , but could not be classified to any recognized species according biochemical tests results. Therefore, a phylogenetic study based on 16S rRNA, 23S rRNA, 60 kDa heat-shock protein (60) and gyrase subunit B () genes was conducted. The 16S rRNA gene sequence (1468 bp) analysis showed that strain 80(YS1) was most closely related to (96.7 % similarity). The 23S rRNA gene sequence (2879 bp) analysis showed that the strain was most closely related to (96 % similarity). The complete gene sequence (2325 bp) analysis showed that it was related phylogenetically to (79.4 % similarity) and (79.1 % similarity). Analysis of the partial sequence of the gene of strain 80(YS1) showed closest similarity to the sequences of (82 %) and (81 %), respectively. However, there was no sequence of available for analysis. The data of morphological, biochemical and phylogenetic characteristics all supported that this strain represents a novel species. The name sp. nov. is proposed for this novel species with the type strain 80(YS1) ( = CGMCC 1.12864 = DSM 28742)

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2015-06-01
2020-01-17
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