1887

Abstract

Four Gram-positive-staining, microaerophilic, non-spore-forming, fructose-6-phosphate phosphoketolase-positive bacterial strains were isolated from a faecal sample of a 5-year-old ring-tailed lemur (). The strains showed a peculiar morphology, resembling a small coiled snake, a ring shape, or forming a little ‘Y’ shape. The isolated strains appeared identical, and LMC 13 was chosen as a representative strain and characterized further. Strain LMC 13 showed an A3β peptidoglycan type, similar to that found in . The DNA base composition was 57.2 mol% G+C. Almost-complete 16S rRNA, , , , , , and gene sequences were obtained, and phylogenetic relationships were determined. Comparative analysis of 16S rRNA gene sequences showed that strain LMC 13 showed the highest similarity to subsp. ATCC 27533 (96.65 %) and DSM 23967 (96.64 %). Strain LMC 13 was located in an actinobacterial cluster and was more closely related to the genus than to other genera in the . On the basis of these results, strain LMC 13 represents a novel species within the genus , for which the name sp. nov. is proposed; the type strain is LMC 13 ( = DSM 28807 = JCM 30168).

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2015-06-01
2019-12-05
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References

  1. Biavati B. , Mattarelli P. . ( 2012; ). Genus Bifidobacterium . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 5, pp. 171–206. Edited by Whitman W. , Goodfellow M. , Kämpfer P. , Busse H.-J. , Trujillo M. E. , Ludwig W. , Suzuki K. , Parte A. . . New York:: Springer;.
    [Google Scholar]
  2. Campbell J. L. , Eisemann J. H. , Williams C. V. , Glenn K. M. . ( 2000; ). Description of the gastrointestinal tract of five lemur species: Propithecus tattersalli, Propithecus verreauxi coquereli, Varecia variegata, Hapalemur griseus, and Lemur catta . . Am J Primatol 52:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cavalli-Sforza L. L. , Edwards A. W. . ( 1967; ). Phylogenetic analysis. Models and estimation procedures. . Am J Hum Genet 19:, 233–257.[PubMed]
    [Google Scholar]
  5. Endo A. , Futagawa-Endo Y. , Schumann P. , Pukall R. , Dicks L. M. T. . ( 2012; ). Bifidobacterium reuteri sp. nov., Bifidobacterium callitrichos sp. nov., Bifidobacterium saguini sp. nov., Bifidobacterium stellenboschense sp. nov. and Bifidobacterium biavatii sp. nov. isolated from faeces of common marmoset (Callithrix jacchus) and red-handed tamarin (Saguinus midas). . Syst Appl Microbiol 35:, 92–97. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Gould L. . ( 2006; ). Lemur catta ecology: what we know and what we need to know. . In Lemurs: Ecology and Adaptation, pp. 255–274. Edited by Gould L. , Sauther M. L. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  8. Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.
  9. Hoyles L. , Inganäs E. , Falsen E. , Drancourt M. , Weiss N. , McCartney A. L. , Collins M. D. . ( 2002; ). Bifidobacterium scardovii sp. nov., from human sources. . Int J Syst Evol Microbiol 52:, 995–999. [CrossRef] [PubMed]
    [Google Scholar]
  10. Jian W. , Zhu L. , Dong X. . ( 2001; ). New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences. . Int J Syst Evol Microbiol 51:, 1633–1638. [CrossRef] [PubMed]
    [Google Scholar]
  11. Jolly A. , Sussman R. W. , Koyama N. , Rasamimanana H. . (editors) ( 2006; ). Ringtailed Lemur Biology: Lemur catta in Madagascar. New York:: Springer;. [CrossRef]
    [Google Scholar]
  12. Katoh K. , Standley D. M. . ( 2013; ). mafft multiple sequence alignment software version 7: improvements in performance and usability. . Mol Biol Evol 30:, 772–780. [CrossRef] [PubMed]
    [Google Scholar]
  13. Killer J. , Kopečný J. , Mrázek J. , Rada V. , Benada O. , Koppová I. , Havlík J. , Straka J. . ( 2009; ). Bifidobacterium bombi sp. nov., from the bumblebee digestive tract. . Int J Syst Evol Microbiol 59:, 2020–2024. [CrossRef] [PubMed]
    [Google Scholar]
  14. Killer J. , Kopečný J. , Mrázek J. , Havlík J. , Koppová I. , Benada O. , Rada V. , Kofroňová O. . ( 2010; ). Bombiscardovia coagulans gen. nov., sp. nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of bumblebees. . Syst Appl Microbiol 33:, 359–366. [CrossRef] [PubMed]
    [Google Scholar]
  15. Killer J. , Kopečný J. , Mrázek J. , Koppová I. , Havlík J. , Benada O. , Kott T. . ( 2011; ). Bifidobacterium actinocoloniiforme sp. nov. and Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract. . Int J Syst Evol Microbiol 61:, 1315–1321. [CrossRef] [PubMed]
    [Google Scholar]
  16. Killer J. , Havlik J. , Bunesova V. , Vlkova E. , Benada O. . ( 2014; ). Pseudoscardovia radai sp. nov., a representative of the family Bifidobacteriaceae isolated from the digestive tract of a wild pig (Sus scrofa scrofa). . Int J Syst Evol Microbiol 64:, 2932–2938. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kim B. J. , Kim H.-Y. , Yun Y.-J. , Kim B.-J. , Kook Y.-H. . ( 2010; ). Differentiation of Bifidobacterium species using partial RNA polymerase β-subunit (rpoB) gene sequences. . Int J Syst Evol Microbiol 60:, 2697–2704.[PubMed] [CrossRef]
    [Google Scholar]
  18. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  19. Martens M. , Dawyndt P. , Coopman R. , Gillis M. , De Vos P. , Willems A. . ( 2008; ). Advantages of multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus Ensifer (including former Sinorhizobium). . Int J Syst Evol Microbiol 58:, 200–214. [CrossRef] [PubMed]
    [Google Scholar]
  20. Mattarelli P. , Biavati B. . ( 2014; ). The genera Bifidobacterium, Parascardovia and Scardovia . . In The Lactic Acid Bacteria: Biodiversity and Taxonomy, pp. 509–541. Edited by Holzapfel W. H. , Wood B. J. B. . . Chichester:: Wiley;. [CrossRef]
    [Google Scholar]
  21. Matteuzzi D. , Crociani F. , Zani G. , Trovatelli L. D. . ( 1971; ). Bifidobacterium suis n. sp.: a new species of the genus Bifidobacterium isolated from pig feces. . Z Allg Mikrobiol 11:, 387–395. [CrossRef] [PubMed]
    [Google Scholar]
  22. McWilliam H. , Li W. , Uludag M. , Squizzato S. , Park Y. M. , Buso N. , Cowley A. P. , Lopez R. . ( 2013; ). Analysis Tool Web Services from the EMBL-EBI. . Nucleic Acids Res 41: (Web Server issue), W597–W600. [CrossRef] [PubMed]
    [Google Scholar]
  23. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  24. Modesto M. , Michelini S. , Stefanini I. , Ferrara A. , Tacconi S. , Biavati B. , Mattarelli P. . ( 2014; ). Bifidobacterium aesculapii sp. nov., from the faeces of the baby common marmoset (Callithrix jacchus). . Int J Syst Evol Microbiol 64:, 2819–2827. [CrossRef] [PubMed]
    [Google Scholar]
  25. Orban J. I. , Patterson J. A. . ( 2000; ). Modification of the phosphoketolase assay for rapid identification of bifidobacteria. . J Microbiol Methods 40:, 221–224. [CrossRef] [PubMed]
    [Google Scholar]
  26. Pineiro M. , Stanton C. . ( 2007; ). Probiotic bacteria: legislative framework – requirements to evidence basis. . J Nutr 137: (Suppl. 2), 850S–853S.[PubMed]
    [Google Scholar]
  27. Rossi M. , Altomare L. , Gonzàlez Vara y Rodriguez A. , Brigidi P. , Matteuzzi D. . ( 2000; ). Nucleotide sequence, expression and transcriptional analysis of the Bifidobacterium longum MB 219 lacZ gene. . Arch Microbiol 174:, 74–80. [CrossRef] [PubMed]
    [Google Scholar]
  28. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  29. Scardovi V. . ( 1986; ). Genus Bifidobacterium . . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 1418–1434. Edited by Sharpe M. E. , Holt J. G. , Sneath P. H. A. , Mair N. S. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  30. Schumann P. . ( 2011; ). Peptidoglycan structure. . Methods Microbiol 38:, 101–129. [CrossRef]
    [Google Scholar]
  31. Talavera G. , Castresana J. . ( 2007; ). Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. . Syst Biol 56:, 564–577. [CrossRef] [PubMed]
    [Google Scholar]
  32. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  33. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013; ). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30:, 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  34. Turroni F. , van Sinderen D. , Ventura M. . ( 2011; ). Genomics and ecological overview of the genus Bifidobacterium . . Int J Food Microbiol 149:, 37–44. [CrossRef] [PubMed]
    [Google Scholar]
  35. Turroni F. , Ventura M. , Buttó L. F. , Duranti S. , O’Toole P. W. , Motherway M. O. , van Sinderen D. . ( 2014; ). Molecular dialogue between the human gut microbiota and the host: a Lactobacillus and Bifidobacterium perspective. . Cell Mol Life Sci 71:, 183–203. [CrossRef] [PubMed]
    [Google Scholar]
  36. Ventura M. , Meylan V. , Zink R. . ( 2003; ). Identification and tracing of Bifidobacterium species by use of enterobacterial repetitive intergenic consensus sequences. . Appl Environ Microbiol 69:, 4296–4301. [CrossRef] [PubMed]
    [Google Scholar]
  37. Ventura M. , van Sinderen D. , Fitzgerald G. F. , Zink R. . ( 2004; ). Insights into the taxonomy, genetics and physiology of bifidobacteria. . Antonie van Leeuwenhoek 86:, 205–223. [CrossRef] [PubMed]
    [Google Scholar]
  38. Ventura M. , Canchaya C. , Del Casale A. , Dellaglio F. , Neviani E. , Fitzgerald G. F. , van Sinderen D. . ( 2006; ). Analysis of bifidobacterial evolution using a multilocus approach. . Int J Syst Evol Microbiol 56:, 2783–2792. [CrossRef] [PubMed]
    [Google Scholar]
  39. Ventura M. , Canchaya C. , Tauch A. , Chandra G. , Fitzgerald G. F. , Chater K. F. , van Sinderen D. . ( 2007; ). Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum. . Microbiol Mol Biol Rev 71:, 495–548. [CrossRef] [PubMed]
    [Google Scholar]
  40. Ventura M. , Turroni F. , Motherway M. O. , MacSharry J. , van Sinderen D. . ( 2012; ). Host-microbe interactions that facilitate gut colonization by commensal bifidobacteria. . Trends Microbiol 20:, 467–476. [CrossRef] [PubMed]
    [Google Scholar]
  41. Watanabe K. , Makino H. , Sasamoto M. , Kudo Y. , Fujimoto J. , Demberel S. . ( 2009; ). Bifidobacterium mongoliense sp. nov., from airag, a traditional fermented mare’s milk product from Mongolia. . Int J Syst Evol Microbiol 59:, 1535–1540. [CrossRef] [PubMed]
    [Google Scholar]
  42. Yildirim S. , Yeoman C. J. , Sipos M. , Torralba M. , Wilson B. A. , Goldberg T. L. , Stumpf R. M. , Leigh S. R. , White B. A. , Nelson K. E. . ( 2010; ). Characterization of the fecal microbiome from non-human wild primates reveals species specific microbial communities. . PLoS ONE 5:, e13963. [CrossRef] [PubMed]
    [Google Scholar]
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