1887

Abstract

A novel, mesophilic, obligately anaerobic, acetate-oxidizing, dissimilatory iron-, sulfur-, and manganese-reducing bacterium, designated strain ICBM, was obtained from an active, coalbed methane gas well in Indiana, USA. Strain ICBM was a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium that was rich in -type cytochromes and formed red colonies in solid medium. Strain ICBM conserved energy to support growth from the oxidation of acetate, propionate, pyruvate, malate, fumarate, succinate and -lactate, concomitant with dissimilatory iron reduction. Strain ICBM fermented fumarate yielding succinate and acetate. Strain ICBM was able to grow in the temperature range of 10 °C to 37 °C, NaCl concentration range of 0 to 1.2 M, and pH range of 6.5 to 8.0. The physiological characteristics of strain ICBM indicated that it belongs to the cluster. The G+C content of its genomic DNA was 61.2 mol%. The predominant cellular fatty acids were C (39.3 %), Cω7 and/or iso-C 2-OH (36.6 %). The closest cultured phylogenetic relative of strain ICBM was BB1 with only 95 % 16S rRNA gene sequence similarity. This confirmed that strain ICBM is affiliated with the genus . On the basis of phenotypic and genotypic differences between strain ICBM and other taxa of the genus, strain ICBM represents a novel species for which the name sp. nov. is proposed (type strain ICBM = DSM 29759 = JCM 30471). Strain ICBM is the first Fe(III)-, S-, and Mn(IV)-reducing bacterium that was isolated from a coal bed.

Funding
This study was supported by the:
  • , the Geobiology and Low-Temperature Geochemistry program of the National Science Foundation , (Award EAR – 1349072)
  • , School of Public and Environmental Affairs, Indiana University
  • , Vietnam Education Foundation
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2015-05-01
2021-03-08
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