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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 65, Issue Pt_5

sp. nov., isolated from a brown alga reservoir Free

Abstract

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped or ovoid bacterial strain, designated W-BA7, was isolated from a brown alga reservoir on the South Sea in South Korea. This strain grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of approximately 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain W-BA7 belonged to the genus , clustering coherently with the type strain of showing 99.3 % sequence similarity. Strain W-BA7 exhibited 16S rRNA gene sequence similarity of 93.4–98.6 % to the type strains of the other species of the genus . Strain W-BA7 contained MK-6 as the predominant menaquinone and iso-C 3-OH and anteiso-C as the major fatty acids. The major polar lipids of strain W-BA7 were phosphatidylethanolamine, three unidentified lipids, one unidentified glycolipid, one unidentified aminolipid and one unidentified aminophospholipid. The DNA G+C content of strain W-BA7 was 31.9 mol%, and the mean DNA–DNA relatedness with the type strains of four phylogenetically related species of the genus was 12–27 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain W-BA7 is separated from recognized species of the genus . On the basis of the data presented, strain W-BA7 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W-BA7 ( = KCTC 42175 = CECT 8670).

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Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE)
  • Program for Collection, Management and Utilization of Biological Resources from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea (Award NRF-2013M3A9A5075953)
© 2015 IUMS
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2015-05-01
2025-01-26
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References

  1. Barrow G. I., Feltham R. K. A. ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  2. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
     [Google Scholar]
  3. Bernardet J.-F. ( 2011 ). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 106–111. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. . New York:: Springer;.
     [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  5. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 1861–1868.[PubMed]
    [Google Scholar]
  6. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 1997–2006. [View Article] [PubMed]
    [Google Scholar]
  7. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957 ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49, 25–68. [View Article]
    [Google Scholar]
  8. Embley T. M., Wait R. ( 1994 ). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O’Donnell A. G. . Chichester:: Wiley;.
     [Google Scholar]
  9. Euzéby J. P. ( 1997 ). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47, 590–592. [View Article] [PubMed]
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  11. Fukui Y., Abe M., Kobayashi M., Saito H., Oikawa H., Yano Y., Satomi M. ( 2013 ). Polaribacter porphyrae sp. nov., isolated from the red alga Porphyra yezoensis, and emended descriptions of the genus Polaribacter and two Polaribacter species. . Int J Syst Evol Microbiol 63, 1665–1672. [View Article] [PubMed]
    [Google Scholar]
  12. Gosink J. J., Woese C. R., Staley J. T. ( 1998 ). Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga-Flavobacterium-Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov.. Int J Syst Bacteriol 48, 223–235. [View Article] [PubMed]
    [Google Scholar]
  13. Hunter I. S. ( 1985 ). Gene cloning in Streptomyces . . In DNA Cloning, vol. 2, p. 32. Edited by Glover D. M. . Oxford:: IRL Press;.
     [Google Scholar]
  14. Hyun D.-W., Shin N.-R., Kim M.-S., Kim P. S., Jung M.-J., Kim J. Y., Whon T. W., Bae J.-W. ( 2014 ). Polaribacter atrinae sp. nov., isolated from the intestine of a comb pen shell, Atrina pectinata . . Int J Syst Evol Microbiol 64, 1654–1661. [View Article] [PubMed]
    [Google Scholar]
  15. Kim B.-C., Oh H. W., Kim H., Park D.-S., Hong S. G., Lee H. K., Bae K. S. ( 2013 ). Polaribacter sejongensis sp. nov., isolated from Antarctic soil, and emended descriptions of the genus Polaribacter, Polaribacter butkevichii and Polaribacter irgensii . . Int J Syst Evol Microbiol 63, 4000–4005. [View Article] [PubMed]
    [Google Scholar]
  16. Komagata K., Suzuki K. ( 1987 ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  17. Lányi B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 1–67. [View Article]
    [Google Scholar]
  18. Lee Y. S., Lee D.-H., Kahng H.-Y., Sohn S. H., Jung J. S. ( 2011 ). Polaribacter gangjinensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61, 1425–1429. [View Article] [PubMed]
    [Google Scholar]
  19. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 1183–1184.[PubMed]
    [Google Scholar]
  20. Li H., Zhang X.-Y., Liu C., Lin C.-Y., Xu Z., Chen X.-L., Zhou B.-C., Shi M., Zhang Y.-Z. ( 2014 ). Polaribacter huanghezhanensis sp. nov., isolated from Arctic fjord sediment, and emended description of the genus Polaribacter . . Int J Syst Evol Microbiol 64, 973–978. [View Article] [PubMed]
    [Google Scholar]
  21. McGuire A. J., Franzmann P. D., McMeekin T. A. ( 1987 ). Flectobacillus glomeratus sp. nov., a curved, nonmotile, pigmented bacterium isolated from Antarctic marine environment. . Syst Appl Microbiol 9, 265–272. [View Article]
    [Google Scholar]
  22. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  23. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Kalinovskaya N. I., Mikhailov V. V., Kim I. S., Bae K. S. ( 2005 ). Polaribacter butkevichii sp. nov., a novel marine mesophilic bacterium of the family Flavobacteriaceae . . Curr Microbiol 51, 408–412. [View Article] [PubMed]
    [Google Scholar]
  24. Nedashkovskaya O. I., Kukhlevskiy A. D., Zhukova N. V. ( 2013 ). Polaribacter reichenbachii sp. nov.: a new marine bacterium associated with the green alga Ulva fenestrata . . Curr Microbiol 66, 16–21. [View Article] [PubMed]
    [Google Scholar]
  25. Park S., Park D.-S., Bae K. S., Yoon J.-H. ( 2014 a). Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. . Int J Syst Evol Microbiol 64, 1378–1383. [View Article] [PubMed]
    [Google Scholar]
  26. Park S., Park J.-M., Jung Y. T., Lee K.-C., Lee J.-S., Yoon J.-H. ( 2014 b). Polaribacter marinivivus sp. nov., a member of the family Flavobacteriaceae isolated from seawater. . Antonie van Leeuwenhoek 106, 1139–1146. [View Article] [PubMed]
    [Google Scholar]
  27. Reichenbach H. ( 1992 ). The order Cytophagales . . In The Prokaryotes, , 2nd edn., pp. 3631–3675. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;. [View Article]
    [Google Scholar]
  28. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. Newark, DE: MIDI Inc.
  29. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  30. Staley J. T. ( 1968 ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95, 1921–1942.[PubMed]
    [Google Scholar]
  31. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  33. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187–194. [View Article] [PubMed]
    [Google Scholar]
  34. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. ( 2003 ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53, 53–57. [View Article] [PubMed]
    [Google Scholar]
  35. Yoon J.-H., Kang S.-J., Oh T.-K. ( 2006 ). Polaribacter dokdonensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 56, 1251–1255. [View Article] [PubMed]
    [Google Scholar]
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Polaribacterundariae sp. nov., isolated from a brown alga reservoir
Int J Syst Evol Microbiol 65, 1679 (2015); https://doi.org/10.1099/ijs.0.000157
/content/journal/ijsem/10.1099/ijs.0.000157
/content/journal/ijsem/10.1099/ijs.0.000157
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