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Abstract

Yellow or orange-to-brown pigmented, ovoid or rod-shaped, Gram-negative staining, aerobic strains PE 4-5 and N5-10 m-1 were isolated from brackish water in Lake Peng Co and fresh to brackish water in Lake Namtso on the Tibetan Plateau, China. Bacteriochlorophyll was produced by the isolates. The predominant cellular fatty acids were C, C and C unsaturated fatty acids, Cω6 (55.3 %), Cω8 (13.0 %) and Cω7 (10.4 %) for PE 4-5 and Cω7 (54.7 %) and Cω7 (18.0 %) for N5-10 m-1. The polar lipid profiles of strains PE 4-5 and N5-10 m-1 were composed of diphosphatidylglycerol, phosphatidylcholine (not detected in N5-10 m-1), phosphatidyldimethylethanolamine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, sphingoglycolipid and an unknown phospholipid. The predominant respiratory quinone was ubiquinone Q10 and the DNA G+C content was 66.0 mol% for both strains. The16S rRNA gene sequence of strain PE 4-5 shared 99.0 % similarity with that of N5-10 m-1, and 97.56 % similarity with those of LMG 17322 and DSM 9006, respectively. The DNA–DNA hybridization relatedness between strains PE 4-5 and N5-10 m-1 was 79.0±1.0 %, but below 70 % with the type strains in the genus . Based on the variability of phylogenetic and phenotypic characteristics, the isolates should be classified as representatives of a novel species of the genus ; the name sp. nov. is proposed. The type strain is PE 4-5 ( = JCM 30179 = CGMCC 1.12851).

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2015-05-01
2019-12-14
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