1887

Abstract

A Gram-staining-negative bacterium, strain TS-T86, was isolated from Lake Tuosu, a saline lake (salinity 5.4 %, w/w) in Qaidam basin, China. Its taxonomic position was determined by using a polyphasic approach. Strain TS-T86 was strictly heterotrophic, aerobic and catalase- and oxidase-positive. Cells were non-spore-forming, non-motile rods, 0.4–0.6 µm wide and 1.2–2.3 µm long. Growth was observed in the presence of 0–9.0 % (w/v) NaCl (optimum, 2.0 %), at 4–35 °C (optimum, 25 °C) and at pH 7.0–10.5 (optimum, pH 8.5–9.0). Strain TS-T86 contained MK-7 as the predominant respiratory quinone. The major fatty acids (>10 %) were iso-C G, iso-C, iso-Cω9, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6). The polar lipids consisted of phosphatidylethanolamine, an unknown phospholipid, six unidentified aminolipids and two uncharacterized lipids. The DNA G+C content was 35 mol% ( ). Phylogenetic trees based on 16S rRNA gene sequences showed that strain TS-T86 was associated with the genus , and showed the highest sequence similarity to BA134 (98.5 %) and then to No.164 (95.7 %) and CC-SAL-25 (95.3 %). DNA–DNA relatedness of strain TS-T86 to DSM 15883 was 32±3 %. It is concluded that strain TS-T86 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TS-T86 ( = CGMCC 1.12479 = JCM 19468).

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2015-05-01
2019-10-23
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