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Abstract

A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, yellow bacterium designated strain KD53, was isolated from a culture of the alga from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD53 was a member of the Roseobacter clade within the family , forming a distinct lineage with species of the genus . The 16S rRNA gene sequence similarities between strain KD53 and other strains examined were all less than 97.0 %. Strain KD53 was found to grow optimally at 28 °C, at pH 7.5–8.0 and in the presence of 3 % (w/v) NaCl. The dominant fatty acids of strain KD53 were Cω6 and/or Cω7, C and C. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 65 mol% and the major respiratory quinone was ubiquinone 10 (Q-10). On the basis of phenotypic data and phylogenetic inference, strain KD53 represents a novel member of the genus , then the name sp. nov. is proposed. The type strain is KD53 ( = MCCC 1K00273 = KCTC 42178).

Funding
This study was supported by the:
  • Joint Project of the National Natural Science Foundation of China (NSFC) and Shandong province, Marine Ecology and Environmental Sciences (Award U1406403)
  • Public Science and Technology Research Funds Projects of Ocean (Award 201305016)
  • National Natural Science Foundation of China (Award 41476095 and 41376119)
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2015-05-01
2021-04-22
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