1887

Abstract

A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, yellow bacterium designated strain KD53, was isolated from a culture of the alga from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD53 was a member of the Roseobacter clade within the family , forming a distinct lineage with species of the genus . The 16S rRNA gene sequence similarities between strain KD53 and other strains examined were all less than 97.0 %. Strain KD53 was found to grow optimally at 28 °C, at pH 7.5–8.0 and in the presence of 3 % (w/v) NaCl. The dominant fatty acids of strain KD53 were Cω6 and/or Cω7, C and C. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 65 mol% and the major respiratory quinone was ubiquinone 10 (Q-10). On the basis of phenotypic data and phylogenetic inference, strain KD53 represents a novel member of the genus , then the name sp. nov. is proposed. The type strain is KD53 ( = MCCC 1K00273 = KCTC 42178).

Funding
This study was supported by the:
  • Joint Project of the National Natural Science Foundation of China (NSFC) and Shandong province, Marine Ecology and Environmental Sciences (Award U1406403)
  • Public Science and Technology Research Funds Projects of Ocean (Award 201305016)
  • National Natural Science Foundation of China (Award 41476095 and 41376119)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000146
2015-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/5/1617.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000146&mimeType=html&fmt=ahah

References

  1. Ausubel F., Brent R., Kingston R. E., Moore D., Seidman J., Smith J. K., Struhl K. . (editors) ( 1995 ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  3. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 18611868.[PubMed]
    [Google Scholar]
  4. Buchan A., González J. M., Moran M. A. ( 2005 ). Overview of the marine roseobacter lineage. . Appl Environ Microbiol 71, 56655677. [View Article] [PubMed]
    [Google Scholar]
  5. DeLong E. F. ( 1992 ). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89, 56855689. [View Article] [PubMed]
    [Google Scholar]
  6. Gonzalez J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B. ( 1997 ). Sagittula stellata gen. nov., sp. nov., a lignin-transforming bacterium from a coastal environment. . Int J Syst Bacteriol 47, 773780. [View Article] [PubMed]
    [Google Scholar]
  7. Hwang C. Y., Cho B. C. ( 2008 ). Ponticoccus litoralis gen. nov., sp. nov., a marine bacterium in the family Rhodobacteraceae . . Int J Syst Evol Microbiol 58, 13321338. [View Article] [PubMed]
    [Google Scholar]
  8. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  9. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Braker G., Hirsch P. ( 1998 ). Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho Lake. . Int J Syst Bacteriol 48, 13631372. [View Article] [PubMed]
    [Google Scholar]
  10. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  11. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  12. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  13. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  14. Su J., Zhou Y., Lai Q., Li X., Dong P., Yang X., Zhang B., Zhang J., Zheng X. et al. ( 2013 ). Sinobacterium caligoides gen. nov., sp. nov., a new member of the family Oceanospirillaceae isolated from the South China Sea, and emended description of Amphritea japonica . . Int J Syst Evol Microbiol 63, 20952100. [View Article] [PubMed]
    [Google Scholar]
  15. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  16. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  17. Wagner-Döbler I., Rheims H., Felske A., Pukall R., Tindall B. J. ( 2003 ). Jannaschia helgolandensis gen. nov., sp. nov., a novel abundant member of the marine Roseobacter clade from the North Sea. . Int J Syst Evol Microbiol 53, 731738. [View Article] [PubMed]
    [Google Scholar]
  18. Zheng Q., Chen C., Yan X.-J., Wang Y.-N., Zeng Y.-H., Hao L.-K., He W.-H., Jiao N.-Z. ( 2010 ). Mameliella alba gen. nov., sp. nov., a marine bacterium of the Roseobacter clade in the order Rhodobacterales . . Int J Syst Evol Microbiol 60, 953957. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000146
Loading
/content/journal/ijsem/10.1099/ijs.0.000146
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error