1887

Abstract

Two pinkish-red, Gram-stain-negative, non-motile aerobic bacterial strains (MCC P1 and MCC P2), capable of growing at low temperatures (15 °C), were isolated from water of a saline lake located in the western Himalayas of India. The strains were capable of growth in the presence of 0–2.0 % NaCl and at pH 6.5–9.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed the closest similarity of 96.3 % to the type strain of the only species of the genus , CCTCC AB 208084. Strains MCC P1 and MCC P2 shared 99.0 % 16S rRNA gene sequence similarity and 88.6 % DNA–DNA relatedness. The major cellular fatty acids were iso-C, Cω6, summed feature 3 (Cω6/Cω7) and summed feature 4 (anteiso-C B/iso-C I). Predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The respiratory quinone was MK-7. The DNA G+C content of the strains was 52.6–52.8 mol%. Based on morphological, physiological, chemotaxonomical and molecular characteristics, strains MCC P1 and MCC P2 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MCC P1 ( = MCC 2268 = CCTCC AB 2013351).

Funding
This study was supported by the:
  • , Department of Biotechnology (DBT, Govt. of India) , (Award BT/PR10054/NDB/52/94/2007)
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2015-05-01
2021-02-25
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References

  1. Abaydulla G., Luo X., Shi J., Peng F., Liu M., Wang Y., Dai J., Fang C. ( 2012 ). Rufibacter tibetensis gen. nov., sp. nov., a novel member of the family Cytophagaceae isolated from soil. . Antonie van Leeuwenhoek 101, 725731. [CrossRef] [PubMed]
    [Google Scholar]
  2. Britton, G., Liaaen-Jensen, S. & Pfander, H. (eds.) (2004). Carotenoids: Handbook. Basel: Birkhauser Verlag.
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cruickshank R. (editor) ( 1965 ). Medical Microbiology: A Guide to the Laboratory Diagnosis and Control of Infection, , 11th edn.. Edinburgh:: E & S Livingstone;.
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [CrossRef] [PubMed]
    [Google Scholar]
  6. Dimitriu P. A., Shukla S. K., Conradt J., Márquez M. C., Ventosa A., Maglia A., Peyton B. M., Pinkart H. C., Mormile M. R. ( 2005 ). Nitrincola lacisaponensis gen. nov., sp. nov., a novel alkaliphilic bacterium isolated from an alkaline, saline lake. . Int J Syst Evol Microbiol 55, 22732278. [CrossRef] [PubMed]
    [Google Scholar]
  7. Gillis M., De Ley J., De Cleene M. ( 1970 ). The determination of molecular weight of bacterial genome DNA from renaturation rates. . Eur J Biochem 12, 143153. [CrossRef] [PubMed]
    [Google Scholar]
  8. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770773. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kates M. ( 1972 ). Techniques of Lipidology. New York:: Elsevier;.
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721.[PubMed] [CrossRef]
    [Google Scholar]
  11. King, E. O., Ward, M. K. & Raney, D. E. (1954). Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44, 301–307.
  12. Loveland-Curtze J., Miteva V. I., Brenchley J. E. ( 2011 ). Evaluation of a new fluorimetric DNA–DNA hybridization method. . Can J Microbiol 57, 250255. [CrossRef] [PubMed]
    [Google Scholar]
  13. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208218. [CrossRef]
    [Google Scholar]
  14. Oren A., Duker S., Ritter S. ( 1996 ). The polar lipid composition of Walsby’s square bacterium. . FEMS Microbiol Lett 138, 135140. [CrossRef]
    [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. ( 1989 ). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  16. Sasser M. ( 1990 ). Identification of bacteria through fatty acid analysis. . In Methods in Phytobacteriology, pp. 199204. Edited by Klement Z., Rudolph K., Sands D. . Budapest:: Akademiai Kiado;.
    [Google Scholar]
  17. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Biology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  18. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. ( 2001 ). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51, 16391652. [CrossRef] [PubMed]
    [Google Scholar]
  19. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. ( 1983 ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54, 3136. [CrossRef]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  21. Tindall B. J. ( 1990 a). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [CrossRef]
    [Google Scholar]
  22. Tindall B. J. ( 1990 b). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [CrossRef]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  24. Yeates C., Gillings M. R., Davison A. D., Altavilla N., Veal D. A. ( 1997 ). PCR amplification of crude microbial DNA extracted from soil. . Lett Appl Microbiol 25, 303307. [CrossRef] [PubMed]
    [Google Scholar]
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