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Volume 65, Issue Pt_5

sp. nov., isolated from a high-altitude saline lake Free

Abstract

Two pinkish-red, Gram-stain-negative, non-motile aerobic bacterial strains (MCC P1 and MCC P2), capable of growing at low temperatures (15 °C), were isolated from water of a saline lake located in the western Himalayas of India. The strains were capable of growth in the presence of 0–2.0 % NaCl and at pH 6.5–9.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed the closest similarity of 96.3 % to the type strain of the only species of the genus , CCTCC AB 208084. Strains MCC P1 and MCC P2 shared 99.0 % 16S rRNA gene sequence similarity and 88.6 % DNA–DNA relatedness. The major cellular fatty acids were iso-C, Cω6, summed feature 3 (Cω6/Cω7) and summed feature 4 (anteiso-C B/iso-C I). Predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The respiratory quinone was MK-7. The DNA G+C content of the strains was 52.6–52.8 mol%. Based on morphological, physiological, chemotaxonomical and molecular characteristics, strains MCC P1 and MCC P2 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MCC P1 ( = MCC 2268 = CCTCC AB 2013351).

  • Published Online:
Funding
This study was supported by the:
  • Department of Biotechnology (DBT, Govt. of India) (Award BT/PR10054/NDB/52/94/2007)
© 2015 IUMS
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2015-05-01
2024-04-20
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Rufibacter immobilis sp. nov., isolated from a high-altitude saline lake
Int J Syst Evol Microbiol 65, 1592 (2015); https://doi.org/10.1099/ijs.0.000144
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