1887

Abstract

A Gram-stain-negative, rod-shaped and non-motile strain, designated PAMC 27266, was isolated from deep-sea sediment of the Arctic Ocean. Analysis of the 16S rRNA gene sequence of strain PAMC 27266 showed closest affiliation with the genus . Phylogenetic analyses revealed that strain PAMC 27266 formed a robust clade with CNI-3, with which it shared 98.9 % 16S rRNA gene sequence similarity. Genomic relatedness analyses based on the average nucleotide identity and genome-to-genome distance showed that strain PAMC 27266 is clearly distinguished from . Cells of strain PAMC 27266 grew optimally at 15 °C and pH 6.5–7.5 in the presence of 3.5 % (w/v) sea salts. The major polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids and two unidentified lipids. The only respiratory quinone was menaquinone-6. The major cellular fatty acids (>10 %) were Cω6 and/or Cω7 and C. The genomic DNA G+C content was 37.9 mol%. Based on the phylogenetic, genomic, chemotaxonomic and phenotypic data presented, we propose strain PAMC 27266 ( = KCCM 43038 = JCM 19894) as the type strain of a novel species, with the name sp. nov.

Funding
This study was supported by the:
  • Korea Polar Research Institute (Award PE15062 and PE15080)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000138
2015-05-01
2021-04-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/5/1567.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000138&mimeType=html&fmt=ahah

References

  1. Anzai Y., Kudo Y., Oyaizu H. ( 1997 ). The phylogeny of the genera Chryseomonas, Flavimonas, and Pseudomonas supports synonymy of these three genera. . Int J Syst Bacteriol 47, 249251. [CrossRef] [PubMed]
    [Google Scholar]
  2. Auch A. F., von Jan M., Klenk H. P., Göker M. ( 2010 ). Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. . Stand Genomic Sci 2, 117134. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 19972006. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cappuccino J. G., Sherman N. ( 2002 ). Microbiology: a Laboratory Manual, , 6th edn.. Menlo Park, CA:: Benjamin/Cummings;.
    [Google Scholar]
  5. Collins M. D. ( 1985 ). Analysis of isoprenoid quinones. . Methods Microbiol 18, 329366. [CrossRef]
    [Google Scholar]
  6. Englen M. D., Kelley L. C. ( 2000 ). A rapid DNA isolation procedure for the identification of Campylobacter jejuni by the polymerase chain reaction. . Lett Appl Microbiol 31, 421426. [CrossRef] [PubMed]
    [Google Scholar]
  7. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. ( 2007 ). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. . Int J Syst Evol Microbiol 57, 8191. [CrossRef] [PubMed]
    [Google Scholar]
  8. Hansen G. H., Sørheim R. ( 1991 ). Improved method for phenotypical characterization of marine bacteria. . J Microbiol Methods 13, 231241. [CrossRef]
    [Google Scholar]
  9. Hwang C. Y., Cho B. C. ( 2008 ). Cohaesibacter gelatinilyticus gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order Rhizobiales, and proposal of Cohaesibacteraceae fam. nov.. Int J Syst Evol Microbiol 58, 267277. [CrossRef] [PubMed]
    [Google Scholar]
  10. Hwang C. Y., Kim M. H., Bae G. D., Zhang G. I., Kim Y. H., Cho B. C. ( 2009 ). Muricauda olearia sp. nov., isolated from crude-oil-contaminated seawater, and emended description of the genus Muricauda . . Int J Syst Evol Microbiol 59, 18561861. [CrossRef] [PubMed]
    [Google Scholar]
  11. Hwang C. Y., Lee I., Cho Y., Lee Y. M., Baek K., Jung Y.-J., Yang Y. Y., Lee T., Rhee T. S., Lee H. K. ( 2015 ). Rhodococcus aerolatus sp. nov., isolated from subarctic rainwater. . Int J Syst Evol Microbiol 65, 465471. [CrossRef] [PubMed]
    [Google Scholar]
  12. Khan S. T., Nakagawa Y., Harayama S. ( 2006 ). Sediminicola luteus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae . . Int J Syst Evol Microbiol 56, 841845. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  14. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  15. Morita R. Y. ( 1975 ). Psychrophilic bacteria. . Bacteriol Rev 39, 144167.[PubMed]
    [Google Scholar]
  16. Richter M., Rosselló-Móra R. ( 2009 ). Shifting the genomic gold standard for the prokaryotic species definition. . Proc Natl Acad Sci U S A 106, 1912619131. [CrossRef] [PubMed]
    [Google Scholar]
  17. Rosselló-Mora R., Amann R. ( 2001 ). The species concept for prokaryotes. . FEMS Microbiol Rev 25, 3967. [CrossRef] [PubMed]
    [Google Scholar]
  18. Skerman V. B. D. ( 1967 ). A Guide to the Identification of the Genera of Bacteria, , 2nd edn.. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  20. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000138
Loading
/content/journal/ijsem/10.1099/ijs.0.000138
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error