1887

Abstract

A rose, Gram-stain-negative, aerobic, rod-shaped bacterium that was motile by gliding, and designated strain H359, was isolated from radiation-polluted soil (with high Cs) from the Xinjiang Uygur Autonomous Region of PR China and subjected to a polyphasic taxonomic analysis. The isolate grew optimally at 30 °C and pH 7.0. It grew with NaCl up to 4 % (w/v). 16S rRNA gene sequence analysis indicated that strain H359 belonged to the genus , a member of the family , with CCTCC AB 208084 as its closest phylogenetic relative, having 96.1 % 16S rRNA gene sequence similarity to the type strain. Strain H359 contained menaquinone-7 (MK-7) as the predominant menaquinone, and the major fatty acids were iso-C, summed feature 4 (iso-C I and/or anteiso-C B), summed feature 3 (Cω7 and/or Cω6) and Cω5. The polar lipid profile had phosphatidylethanolamine as the major component. The DNA G+C content was 43.9 mol%. Based on phenotypic, genotypic and phylogenetic evidence, strain H359 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H359 ( = CPCC 100615 = KCTC 42217).

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2015-05-01
2019-10-20
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References

  1. Abaydulla G., Luo X. S., Shi J. J., Peng F., Liu M., Wang Y. Q., Dai J., Fang C. X.. ( 2012; ). Rufibacter tibetensis gen. nov., sp. nov., a novel member of the family Cytophagaceae isolated from soil. . Antonie van Leeuwenhoek 101:, 725–731. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bowman J. P.. ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  4. Brettar I., Christen R., Höfle M. G.. ( 2004; ). Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea. . Int J Syst Evol Microbiol 54:, 2335–2341. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cerny G.. ( 1978; ). Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5:, 113–122. [CrossRef]
    [Google Scholar]
  6. Collins M. D.. ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  7. Donachie S. P., Bowman J. P., Alam M.. ( 2004; ). Psychroflexus tropicus sp. nov., an obligately halophilic Cytophaga-Flavobacterium-Bacteroides group bacterium from an Hawaiian hypersaline lake. . Int J Syst Evol Microbiol 54:, 935–940. [CrossRef] [PubMed]
    [Google Scholar]
  8. Felsenstein J.. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  9. Felsenstein J.. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  10. Filippini M., Kaech A., Ziegler U., Bagheri H. C.. ( 2011; a). Fibrisoma limi gen. nov., sp. nov., a filamentous bacterium isolated from tidal flats. . Int J Syst Evol Microbiol 61:, 1418–1424. [CrossRef] [PubMed]
    [Google Scholar]
  11. Filippini M., Svercel M., Laczko E., Kaech A., Ziegler U., Bagheri H. C.. ( 2011; b). Fibrella aestuarina gen. nov., sp. nov., a filamentous bacterium of the family Cytophagaceae isolated from a tidal flat, and emended description of the genus Rudanella Weon et al. 2008. . Int J Syst Evol Microbiol 61:, 184–189. [CrossRef] [PubMed]
    [Google Scholar]
  12. Fitch W. M.. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  13. Frette L., Jørgensen N. O. G., Irming H., Kroer N.. ( 2004; ). Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. . Int J Syst Evol Microbiol 54:, 519–524. [CrossRef] [PubMed]
    [Google Scholar]
  14. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N.. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  15. Groth I., Tan G. Y. A., González J. M., Laiz L., Carlsohn M. R., Schütze B., Wink J., Goodfellow M.. ( 2007; ). Amycolatopsis nigrescens sp. nov., an actinomycete isolated from a Roman catacomb. . Int J Syst Evol Microbiol 57:, 513–519. [CrossRef] [PubMed]
    [Google Scholar]
  16. Guindon S., Gascuel O.. ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52:, 696–704. [CrossRef] [PubMed]
    [Google Scholar]
  17. Jiang F., Xiao M., Chen L., Kan W., Peng F., Dai J., Chang X., Li W., Fang C.. ( 2013; ). Huanghella arctica gen. nov., sp. nov., a bacterium of the family Cytophagaceae isolated from Arctic tundra soil. . Int J Syst Evol Microbiol 63:, 696–702. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kämpfer P., Busse H.-J., Longaric I., Rosselló-Móra R., Galatis H., Lodders N.. ( 2012; ). Pseudarcicella hirudinis gen. nov., sp. nov., isolated from the skin of the medical leech Hirudo medicinalis. . Int J Syst Evol Microbiol 62:, 2247–2251. [CrossRef] [PubMed]
    [Google Scholar]
  19. Kang J. Y., Chun J., Jahng K. Y.. ( 2013; ). Nibribacter koreensis gen. nov., sp. nov., isolated from estuarine water. . Int J Syst Evol Microbiol 63:, 4663–4668. [CrossRef] [PubMed]
    [Google Scholar]
  20. Kimura M.. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  21. Kovács N.. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178:, 703–704. [CrossRef] [PubMed]
    [Google Scholar]
  22. Kroppenstedt R. M.. ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B.. ( 1989; ). Precise measurement of the G?C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  24. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  25. Nakagawa Y.. ( 2011; ). Family I. Cytophagaceae stanier 1940, 630AL.. In Bergey’s Manual of Systemic Bacteriology, vol. 4. pp. 371. Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B... New York:: Springer;
    [Google Scholar]
  26. Reichenbach H.. ( 1989; ). Order I. Cytophagales leadbetter 1974, 99AL. . In Bergey’s Manual of Systematic Bacteriology, vol. 3, pp. 2011–2082. Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G... Baltimore:: Williams and Wilkins;.
    [Google Scholar]
  27. Saitou N., Nei M.. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  28. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  30. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  31. Wang Y., Zhang K. D., Cai F., Zhang L., Tang Y. L., Dai J., Fang C. X.. ( 2010; ). Pontibacter xinjiangensis sp. nov., in the phylum ‘Bacteroidetes’, and reclassification of [Effluviibacter] roseus as Pontibacter roseus comb. nov.. Int J Syst Evol Microbiol 60:, 99–103. [CrossRef] [PubMed]
    [Google Scholar]
  32. Williams S. T., Goodfellow M., Alderson G.. ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL. . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Wil S. T..l iams M. E., Sharpe J. G., Holt.. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  33. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L.. ( 2005; ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef] [PubMed]
    [Google Scholar]
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