sp. nov., isolated from mangrove sediment Free

Abstract

A Gram-stain-negative, Na-requiring bacterial strain, designated B20-1, was isolated from soil of the root system of mangrove forest. Cells were curved rods and motile by means of a polar flagellum. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B20-1 belonged to the genus , sharing highest sequence similarities with IVIA-Po-145 (97.6 %), DSW10-10 (97.0 %) and IVIA-Po-155 (96.9 %). The predominant cellular fatty acids of strain B20-1 were C 3-OH, Cω7, summed feature 3 (Cω7 and/or iso-C 2-OH) and C. Phosphatidylethanolamine and phosphatidylglycerol were identified as the predominant phospholipids. The predominant ubiquinone was Q-8. The genomic DNA G+C content of strain B20-1 was 46.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness, a novel species, sp. nov., is proposed with B20-1 ( = DSM 28136 = LMG 28077) as the type strain.

Funding
This study was supported by the:
  • Ministry of Science and Technology of China (Award 2014FY110500)
  • National Natural Science Foundation of China (Award 31200005 and 31270050)
  • IOCAS (Award 2012IO060103)
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2015-05-01
2024-03-28
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References

  1. Chimetto L. A., Cleenwerck I., Brocchi M., Willems A., De Vos P., Thompson F. L. ( 2011 ). Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis . . Int J Syst Evol Microbiol 61, 11701175. [View Article] [PubMed]
    [Google Scholar]
  2. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  4. Espinosa E., Marco-Noales E., Gómez D., Lucas-Elío P., Ordax M., Garcías-Bonet N., Duarte C. M., Sanchez-Amat A. ( 2010 ). Taxonomic study of Marinomonas strains isolated from the seagrass Posidonia oceanica, with descriptions of Marinomonas balearica sp. nov. and Marinomonas pollencensis sp. nov.. Int J Syst Evol Microbiol 60, 9398. [View Article] [PubMed]
    [Google Scholar]
  5. Huss V. A. R., Festl H., Schleifer K.-H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article] [PubMed]
    [Google Scholar]
  6. Ivanova E. P., Onyshchenko O. M., Christen R., Lysenko A. M., Zhukova N. V., Shevchenko L. S., Kiprianova E. A. ( 2005 ). Marinomonas pontica sp. nov., isolated from the Black Sea. . Int J Syst Evol Microbiol 55, 275279. [View Article] [PubMed]
    [Google Scholar]
  7. Jung Y.-T., Oh T.-K., Yoon J.-H. ( 2012 ). Marinomonas hwangdonensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 62, 20622067. [View Article] [PubMed]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  9. Kumari P., Poddar A., Das S. K. ( 2014 ). Marinomonas fungiae sp. nov., isolated from the coral Fungia echinata from the Andaman Sea. . Int J Syst Evol Microbiol 64, 487494. [View Article] [PubMed]
    [Google Scholar]
  10. Lucas-Elío P., Marco-Noales E., Espinosa E., Ordax M., López M. M., Garcías-Bonet N., Marbà N., Duarte C. M., Sanchez-Amat A. ( 2011 ). Marinomonas alcarazii sp. nov., M. rhizomae sp. nov., M. foliarum sp. nov., M. posidonica sp. nov. and M. aquiplantarum sp. nov., isolated from the microbiota of the seagrass Posidonia oceanica . . Int J Syst Evol Microbiol 61, 21912196. [View Article] [PubMed]
    [Google Scholar]
  11. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [View Article] [PubMed]
    [Google Scholar]
  12. Owen R. J., Pitcher D. ( 1985 ). Current methods for estimating DNA base composition and levels of DNA–DNA hybridization. . In Chemical Methods in Bacterial Systematics, pp. 6793. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  13. Romanenko L. A., Tanaka N., Frolova G. M. ( 2009 ). Marinomonas arenicola sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 59, 28342838. [View Article] [PubMed]
    [Google Scholar]
  14. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  15. Sambrook J., Frisch E. F., Maniatis T. ( 1989 ). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  16. Sanchez-Amat A., Solano F. ( 2005 ). Genus III. Marinomonas Van Landschoot and De Ley 1984, 91VP (Effective publication Van Landschoot and De Ley 1983, 3071). . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, part B, pp. 284289. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . . New York:: Springer;.
    [Google Scholar]
  17. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Süßmuth R., Eberspächer J., Haag R., Springer W. ( 1987 ). Biochemisch-mikrobiologisches Praktikum. Stuttgart:: Georg Thieme Verlag;.
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  20. Tindall B. J. ( 1990 a). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  21. Tindall B. J. ( 1990 b). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [View Article]
    [Google Scholar]
  22. Van Landschoot A., De Ley J. ( 1983 ). Intra- and intergenic similarities of the rRNA cistrons of Alteromonas, Marinomonas (gen. nov.) and some other Gram negative bacteria. . J Gen Microbiol 129, 30573074.
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  24. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989 ). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology 16, 176178 (English translation of Mikrobiologiya).
    [Google Scholar]
  25. Yoon J.-H., Kang S.-J., Oh T.-K. ( 2005 ). Marinomonas dokdonensis sp. nov., isolated from sea water. . Int J Syst Evol Microbiol 55, 23032307. [View Article] [PubMed]
    [Google Scholar]
  26. Zhang D.-C., Margesin R. ( 2014 ). Martelella radicis sp. nov. and Martelella mangrovi sp. nov., isolated from mangrove sediment. . Int J Syst Evol Microbiol 64, 31043108. [View Article] [PubMed]
    [Google Scholar]
  27. Zhang D.-C., Wang H.-X., Liu H.-C., Dong X.-Z., Zhou P.-J. ( 2006 ). Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. . Int J Syst Evol Microbiol 56, 29212925. [View Article] [PubMed]
    [Google Scholar]
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