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Abstract

A Gram-stain-negative heterotrophic bacterium, designated GSD6, capable of growth on aliphatic hydrocarbons as a sole carbon and energy source, was isolated from sea-tidal flat sediment of the Yellow Sea, South Korea. Cells were facultatively aerobic, catalase- and oxidase-positive, motile rods with a single polar flagellum. Growth of strain GSD6 was observed at 4–37 °C (optimum 30 °C), at pH 5.5–9.0 (optimum pH 6.5–7.5) and in the presence of 1–9 % (w/v) NaCl (optimum 2 %). Strain GSD6 contained ubiquinone-8 (Q-8) as the sole isoprenoid quinone and summed feature 3 (comprising Cω7 and/or iso-C 2-OH), C, Cω7, C 10-methyl and Cω8 as the major fatty acids. Phosphatidylethanolamine and phosphatidylglycerol were identified as the major polar lipids. The G+C content of the genomic DNA was 44.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GSD6 formed a phylogenetic lineage with members of the genus . Strain GSD6 was most closely related to E3 with a 16S rRNA gene sequence similarity of 97.4 %, but their DNA–DNA hybridization value was 39.1±7.1 %. On the basis of phenotypic, chemotaxonomic and molecular features, strain GSD6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GSD6 ( = KACC 18129 = JCM 30133). An emended description of the genus is also proposed.

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2015-05-01
2020-01-23
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