1887

Abstract

A polycyclic aromatic hydrocarbons-degrading and acylhomoserine-lactone-producing marine bacterium, designated strain PQ-2, was isolated from marine biofilm collected from a boat shell at a harbour of Zhoushan island in Zhejiang Province, PR China. Strain PQ-2 is Gram-stain-negative, yellow-pigmented, non-motile and short rod-shaped. Optimal growth of strain PQ-2 was observed at 32 °C, at pH 7.0 and in 2 % (w/v) NaCl. The 16S rRNA gene sequence of strain PQ-2 showed highest similarity to E4A9 (96.3 %) followed by MUSC 273 (95.6 %) and H32 (95.6 %). Phylogenetic analysis with all species of the family with validly published names revealed that strain PQ-2 formed a phyletic line with E4A9 that was distinct from other members of the family . The sole respiratory quinone was ubiquinone 10 (Q-10). The predominant fatty acids were Cω7, Cω6 and summed feature 3 (Cω7 and/or iso-C 2-OH). The genomic DNA G+C content was 61.7 mol%. In the polar lipid profile, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, one unidentified phospholipid and one sphingoglycolipid were the major compounds; and another sphingoglycolipid was present in a minor amount. Based on the genotypic and phenotypic data, strain PQ-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PQ-2 ( = CGMCC 1.12805 = NBRC 110381). In addition, emended descriptions for the genus and the species are given.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000132
2015-05-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/5/1531.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000132&mimeType=html&fmt=ahah

References

  1. Dong X. Z., Cai M. Y.. ( 2001; ). Common Manual of Systematic Bacteriology. Beijing:: Science Press;.
    [Google Scholar]
  2. Felsenstein J.. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  3. Felsenstein J.. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  4. Huang Y. L., Ki J. S., Case R. J., Qian P. Y.. ( 2008; ). Diversity and acylhomoserine lactone production among subtidal biofilm forming bacteria. . Aquat Microb Ecol 52:, 185–193. [CrossRef]
    [Google Scholar]
  5. Huang Y. L., Zeng Y. H., Yu Z. L., Zhang J., Feng H., Lin X. C.. ( 2013; ). In silico and experimental methods revealed highly diverse bacteria with quorum sensing and aromatics biodegradation systems–a potential broad application on bioremediation. . Bioresour Technol 148:, 311–316. [CrossRef] [PubMed]
    [Google Scholar]
  6. Huo Y. Y., Xu X. W., Cui H. L., Wu M.. ( 2010; ). Gracilibacillus ureilyticus sp. nov., a halotolerant bacterium from a saline-alkaline soil. . Int J Syst Evol Microbiol 60:, 1383–1386. [CrossRef] [PubMed]
    [Google Scholar]
  7. Jeon Y. S., Lee K., Park S. C., Kim B. S., Cho Y. J., Ha S. M., Chun J.. ( 2014; ). EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. . Int J Syst Evol Microbiol 64:, 689–691. [CrossRef] [PubMed]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R.. ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–132. Edited by Munro H. N... New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kiyohara H., Nagao K., Yana K.. ( 1982; ). Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates. . Appl Environ Microbiol 43:, 454–457.[PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K.. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  12. Mesbah M., Whitman W. B.. ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef] [PubMed]
    [Google Scholar]
  13. Saitou N., Nei M.. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  14. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  15. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  16. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  17. Xu X. W., Wu Y. H., Wang C. S., Wang X. G., Oren A., Wu M.. ( 2009; ). Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae. . Int J Syst Evol Microbiol 59:, 2247–2253. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000132
Loading
/content/journal/ijsem/10.1099/ijs.0.000132
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error