1887

Abstract

A Gram-stain-negative, non-motile, aerobic and coccoid, ovoid or rod-shaped bacterial strain, designated BS-W9, was isolated from a tidal flat of the South Sea, South Korea. Strain BS-W9 grew optimally at 25–30 °C, at pH 7.0–8.0 and in the presence of approximately 2.0 % (w/v) NaCl. Phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain BS-W9 clustered with the type strain of , showing a highest sequence similarity of 97.3 %. It exhibited sequence similarity values of less than 95.6 % to the type strains of other species with validly published names. Strain BS-W9 contained Q-10 as the predominant ubiquinone and Cω7 as the predominant fatty acid. The major polar lipids of strain BS-W9 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The fatty acid and polar lipid profiles of strain BS-W9 were distinguished from those of the type strains of and other phylogenetically related genera. The DNA G+C content of strain BS-W9 was 53.2 mol% and its mean DNA–DNA relatedness value with RA2-3 was 14 %. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain BS-W9 is considered to represent a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is BS-W9 ( = KCTC 42348 = CECT 8726).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000128
2015-05-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/5/1509.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000128&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A.. (editors) ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Baumann P., Baumann L.. ( 1981; ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G... Berlin:: Springer;.
    [Google Scholar]
  3. Buchan A., González J. M., Moran M. A.. ( 2005; ). Overview of the marine roseobacter lineage. . Appl Environ Microbiol 71:, 5665–5677. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y.. ( 1957; ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49:, 25–68. [CrossRef]
    [Google Scholar]
  5. Embley T. M., Wait R.. ( 1994; ). Structural lipids of eubacteria. . In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O’Donnell A. G... Chichester:: John Wiley & Sons;.
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  7. Garrity G. M., Bell J. A., Lilburn T.. ( 2005; ). Order III. Rhodobacterales ord. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2 ( The Proteobacteria), part C, pp 161. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M... New York:: Springer;.
    [Google Scholar]
  8. Garrity G. M., Bell J. A., Lilburn T.. ( 2006; ). Rhodobacterales ord. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published,Validation List no 107. . Int J Syst Evol Microbiol 56:, 1–6. [CrossRef] [PubMed]
    [Google Scholar]
  9. Jung Y.-T., Kim B.-H., Oh T.-K., Yoon J.-H.. ( 2010; ). Pseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description of the genus Pseudoruegeria. . Int J Syst Evol Microbiol 60:, 1177–1181. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kim Y.-O., Park S., Kim H., Park D.-S., Nam B.-H., Kim D.-G., Yoon J.-H.. ( 2014; a). Halocynthiibacter namhaensis gen. nov., sp. nov., a novel alphaproteobacterium isolated from sea squirt Halocynthia roretzi. . Antonie van Leeuwenhoek 105:, 881–889. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim Y.-O., Park S., Nam B.-H., Kim D.-G., Yoon J.-H.. ( 2014; b). Pseudopelagicola gijangensis gen. nov., sp. nov., isolated from the sea squirt Halocynthia roretzi. . Int J Syst Evol Microbiol 64:, 3447–3452. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kim Y.-O., Park S., Nam B.-H., Lee C., Park J.-M., Kim D.-G., Yoon J.-H.. ( 2014; c). Ascidiaceihabitans donghaensis gen. nov., sp. nov., isolated from the golden sea squirt Halocynthia aurantium. . Int J Syst Evol Microbiol 64:, 3970–3975. [CrossRef] [PubMed]
    [Google Scholar]
  13. Komagata K., Suzuki K.. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  14. Lányí B.. ( 1987; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  15. Levring T.. ( 1946; ). Some culture experiments with Ulva and artificial seawater. . Kungl Fysiografiska Sällsk Lund Förhandlingar 16:, 45–56.
    [Google Scholar]
  16. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  17. Park S., Jung Y.-T., Yoon J.-H.. ( 2013; ). Pelagicola litorisediminis sp. nov., a novel alphaproteobacterium isolated from tidal flat sediment. . Antonie van Leeuwenhoek 104:, 103–110. [CrossRef] [PubMed]
    [Google Scholar]
  18. Park S., Park J.-M., Lee K.-C., Bae K. S., Yoon J.-H.. ( 2014; a). Boseongicola aestuarii gen. nov., sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 64:, 2618–2624. [CrossRef] [PubMed]
    [Google Scholar]
  19. Park S., Won S.-M., Kim H., Park D.-S., Yoon J.-H.. ( 2014; b). Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 64:, 2969–2974. [CrossRef] [PubMed]
    [Google Scholar]
  20. Rappé M. S., Vergin K., Giovannoni S. J.. ( 2000; ). Phylogenetic comparisons of a coastal bacterioplankton community with its counterparts in open ocean and freshwater systems. . FEMS Microbiol Ecol 33:, 219–232. [CrossRef] [PubMed]
    [Google Scholar]
  21. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  22. Staley J. T.. ( 1968; ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95:, 1921–1942.[PubMed]
    [Google Scholar]
  23. Tamaoka J., Komagata K.. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  24. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H.. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Lee S. T., Park Y.-H.. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yoon J.-H., Kang K. H., Park Y.-H.. ( 2003; ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53:, 449–454. [CrossRef] [PubMed]
    [Google Scholar]
  27. Yoon J.-H., Kang S.-J., Lee J.-S., Oh T.-K.. ( 2009; a). Lutimaribacter saemankumensis gen. nov., sp. nov., isolated from a tidal flat of the Yellow Sea. . Int J Syst Evol Microbiol 59:, 48–52. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yoon J.-H., Kang S.-J., Lee S.-Y., Oh K.-H., Oh T.-K.. ( 2009; b). Seohaeicola saemankumensis gen. nov., sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 59:, 2675–2679. [CrossRef] [PubMed]
    [Google Scholar]
  29. Yoon J.-H., Kang S.-J., Jung Y.-T., Oh T.-K.. ( 2010; ). Gaetbulicola byunsanensis gen. nov., sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 60:, 196–199. [CrossRef] [PubMed]
    [Google Scholar]
  30. Yoon J.-H., Lee S.-Y., Jung Y.-T., Lee J.-S., Lee K.-C.. ( 2013; a). Litorisediminicola beolgyonensis gen. nov., sp. nov., isolated from a coastal sediment. . Int J Syst Evol Microbiol 63:, 2025–2031. [CrossRef] [PubMed]
    [Google Scholar]
  31. Yoon J.-H., Park S., Jung Y.-T.. ( 2013; b). Aestuariihabitans beolgyonensis gen. nov., sp. nov., a novel alphaproteobacterium isolated from tidal flat sediment. . Antonie van Leeuwenhoek 104:, 217–224. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000128
Loading
/content/journal/ijsem/10.1099/ijs.0.000128
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error