1887

Abstract

A novel bacterial strain, designated DCY94, was isolated from forest soil cultivated with ginseng in Vietnam. The strain was Gram-reaction-negative, facultatively anaerobic, non-motile, rod-shaped and catalase- and oxidase-positive. 16S rRNA gene sequence analysis demonstrated that strain DCY94 was closely related to Zy-3 (97.5 % 16S rRNA gene sequence similarity) and MJ17 (96.9 %). The fatty acid profile of strain DCY94 contained a predominant amount of summed feature 8 (Cω7 and/or Cω6; 88.4 %) and moderate to small quantities of C 3-OH (1.0 %), C 3-OH (2.8 %) and C (5.2 %). Phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and one unidentified glycolipid were major polar lipids; one unidentified aminolipid, one unidentified aminophospholipid, one unidentified phospholipid and four unidentified polar lipids were minor components. The polyamine pattern comprised the major compounds putrescine and spermidine and minor amounts of -homospermidine and spermine. The ubiquinone of the strain was Q-10 and the G+C content of its genomic DNA was 68.3 mol%. All these results support the placement of strain DCY94 within the genus . Levels of DNA–DNA relatedness between strain DCY94 and HAMBI 3106 and KCTC 22480 were 52 and 50 %, respectively. The results of phylogenetic analysis, phenotypic tests, chemotaxonomic characterization and DNA–DNA relatedness studies distinguished strain DCY94 from the closest recognized species of the genus , suggesting that this strain represents a novel species, for which the name sp. nov. is proposed. The type strain is DCY94 ( = KCTC 42086 = JCM 30337).

Funding
This study was supported by the:
  • Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries (Award KIPET no. 313038-03-1-SB010)
  • Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration (Award C0214183)
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2015-05-01
2022-01-21
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