An endophytic actinobacterial strain, CS1-12T, was isolated from a leaf of Costus speciosus. Single spores were observed directly on the substrate mycelia. The cell-wall peptidoglycan of this strain exhibited meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid. Arabinose, glucose, ribose, xylose and rhamnose were detected as whole-cell sugars. The diagnostic phospholipids of this strain were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant menaquinones were MK-10(H6), MK-10(H8), MK-9(H4) and MK-10(H4). The main components of the cellular fatty acids (>10 %) were iso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 72.8 mol%. 16S rRNA gene sequence analysis data showed that strain CS1-12T should be classified in the genus Micromonospora and that it is closely related to Micromonospora fulviviridis DSM 43906T (99.3 %). The physiological, biochemical and chemotaxonomic properties, including the DNA–DNA hybridization results, indicated that strain CS1-12T could be judged a novel species of the genus Micromonospora, for which the name Micromonospora costi sp. nov. is proposed. The type strain is CS1-12T ( = BCC 58124T = NBRC 109518T).
Published Online:
Funding
This study was supported by the:
Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang
Department of Biology and Actinobacterial Research Unit, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria
. . J Gen Microbiol100, 221–230. [View Article][PubMed]
EzakiT.,
HashimotoY.,
YabuuchiE.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol39, 224–229. [View Article]
GaoR.,
LiuC.,
ZhaoJ.,
JiaF.,
YuC.,
YangL.,
WangX.,
XiangW.(2014).Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora
. . Antonie van Leeuwenhoek105, 307–315. [View Article][PubMed]
KämpferP.,
KroppenstedtR. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol42, 989–1005. [View Article]
KawamotoI.(1989). Genus Micromonospora Orskov 1923, 147AL
. . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2442–2450. Edited by
WilliamsS. T.,
SharpeM. E.,
HoltJ. G.
. Baltimore:: Williams & Wilkins;.
KellyK. L.(1964).Inter-Society Color Council – National Bureau of Standard Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
LaneD. J.(1991). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–148. Edited by
StackebrandtE.,
GoodfellowM.
. Chichester:: John Wiley & Sons;.
LechevalierM. P.,
LechevalierH. A.(1980). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy, pp. 227–291. Edited by
DietzA.,
ThayerD. W.
. Arlington, VA:: Society for Industrial Microbiology;.
MinnikinD. E.,
AlshamaonyL.,
GoodfellowM.(1975). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. . J Gen Microbiol88, 200–204. [View Article][PubMed]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2, 233–241. [View Article]
TamaokaJ.(1994). Determination of DNA Base Composition. . In Chemical Methods in Prokaryotic Systematics, pp. 463–470. Edited by
GoodfellowM.,
O’DonnellA. G.
. Chichester:: John Wiley & Sons;.
TamaokaJ.,
KomagataK.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett25, 125–128. [View Article]
UchidaK.,
AidaK. O.(1984). An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. . J Gen Appl Microbiol30, 131–134. [View Article]
VerlanderC. P.(1992). Detection of horseradish peroxidase by colorimetry. . In Nonisotopic DNA Probe Techniques, pp. 185–201. Edited by
KrickaL. J.
. New York:: Academic Press;. [View Article]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E. et al.(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]