1887

Abstract

A Gram-stain-positive, pleomorphic, oxidase-negative, non-motile isolate from the trachea of a white stork from Poland, designated strain 136/3, was subjected to a comprehensive taxonomic investigation. A comparative analysis of the 16S rRNA gene sequence showed highest similarities to , , and (96.0–96.3 %). The quinone system consisted of major amounts of MK-8(H), minor amounts of MK-9(H) and traces of MK-8 and MK-9. The polar lipid profile of strain 136/3 contained phosphatidylinositol and phosphatidylinositol-mannoside as major lipids and phosphatidylglycerol and an acidic glycolipid in moderate amounts. In addition small amounts of diphosphatidylglycerol, a phospholipid, an aminolipid and two lipids of unknown group affiliation were found. The polyamine pattern was composed of the major components spermidine and spermine. Putrescine, 1,3-diaminopropane, cadaverine, -homospermidine and tyramine were found in minor or trace amounts. The diamino acid of the peptidoglycan was -diaminopimelic acid. In the fatty acid profile straight-chain, saturated and mono-unsaturated fatty acids predominated (Cω9, Cω7, C, C). Corynemycolic acids were detected. Physiological traits as well as unique traits of the polar lipid profile and the fatty acid pattern distinguished strain 136/3 from the most closely related species. All these results indicate that strain 136/3 represents a novel species of the genus for which we propose the name sp. nov. The type strain is 136/3 ( = CIP 110778 = CCM 8517 = LMG 28174).

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2015-05-01
2020-01-23
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