1887

Abstract

A Gram-stain-negative, orange-coloured, rod-shaped bacterium, designated strain Th68, was isolated from the intestine of flounder (). The isolate required sea salts for growth. Gliding motility was not observed. Flexirubin-type pigments were present. 16S rRNA gene sequence analysis indicated that strain Th68 represented a distinct phyletic line within the family with less than 96.1 % similarity to members of the recognized genera of the family. The DNA G+C content was 33.0 mol%. The major fatty acids were iso-C, iso-C G, iso-C 3-OH and iso-C 3-OH. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified polar lipids. Menaquinone 6 (MK-6) was the only respiratory quinone. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain Th68 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is Th68 ( = JCM 18637 = KCTC 32141).

Funding
This study was supported by the:
  • , International Science and Technology Cooperation Program of China , (Award 2012DFG31990)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000110
2015-04-01
2020-08-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/4/1347.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000110&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y. ( 2006 ). An introduction to the family Flavobacteriaceae . . In The Prokaryotes, pp. 455480. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. ( 1996 ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Bacteriol 46, 128148. [CrossRef]
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [CrossRef] [PubMed]
    [Google Scholar]
  4. Beveridge T., Lawrence J., Murray R. ( 2007 ). Sampling and staining for light microscopy. . In Methods for General and Molecular Microbiology, 1933. Edited by Reddy C. A., Beveridge T. J., Breznak J. A. , Marzluf G., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  5. Buczolits S., Denner E. B. M., Vybiral D., Wieser M., Kämpfer P., Busse H. J. ( 2002 ). Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov.. Int J Syst Evol Microbiol 52, 445456.[PubMed] [CrossRef]
    [Google Scholar]
  6. Collins M. D., Shah H. N. ( 1984 ). Fatty acid, menaquinone and polar lipid composition of Rothia dentosacariosa . . Arch Microbiol 137, 247249. [CrossRef]
    [Google Scholar]
  7. Einen J., Øvreås L. ( 2006 ). Flaviramulus basaltis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seafloor basalt. Int J Syst Evol Microbiol 56, 2455–2461.
  8. Gonzalez C., Gutierrez C., Ramirez C. ( 1978 ). Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. . Can J Microbiol 24, 710715. [CrossRef] [PubMed]
    [Google Scholar]
  9. Hsu S. C., Lockwood J. L. ( 1975 ). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. . Appl Microbiol 29, 422426.[PubMed]
    [Google Scholar]
  10. Ivanova E. P., Alexeeva Y. V., Flavier S., Wright J. P., Zhukova N. V., Gorshkova N. M., Mikhailov V. V., Nicolau D. V., Christen R. ( 2004 ). Formosa algae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae . . Int J Syst Evol Microbiol 54, 705711. [CrossRef] [PubMed]
    [Google Scholar]
  11. Jung S. Y., Kang S. J., Lee M. H., Lee S. Y., Oh T. K., Yoon J. H. ( 2005 ). Gaetbulibacter saemankumensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 55, 1845–1849.
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  14. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [CrossRef]
    [Google Scholar]
  15. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479, 297306. [CrossRef] [PubMed]
    [Google Scholar]
  16. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  17. Moore E. R. B., Arnscheidt A., Krüger A., Strömpl C., Mau M. ( 1999 ). Simplified protocols for the preparation of genomic DNA from bacterial cultures. . In Molecular Microbial Ecology Manual 1.6.1, pp. 115. Edited by Akkermans A. D. L., van Elsas J. D., de Bruijn F. J. . Dordrecht:: Kluwer;.
    [Google Scholar]
  18. Nedashkovskaya O. I., Kim S. B., Han S. K., Rhee M.-S., Lysenko A. M., Rohde M., Zhukova N. V., Frolova G. M., Mikhailov V. V., Bae K. S. ( 2004 ). Algibacter lectus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from green algae. . Int J Syst Evol Microbiol 54, 12571261. [CrossRef] [PubMed]
    [Google Scholar]
  19. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Bae K. S. ( 2005 ). Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea . . Int J Syst Evol Microbiol 55, 375378. [CrossRef] [PubMed]
    [Google Scholar]
  20. Park S. C., Hwang Y. M., Choe H. N., Baik K. S., Kim H., Seong C. N. ( 2013 ). Algibacter aquimarinus sp. nov., isolated from a marine environment, and reclassification of Pontirhabdus pectinivorans as Algibacter pectinivorans comb. nov.. Int J Syst Evol Microbiol 63, 20382042. [CrossRef] [PubMed]
    [Google Scholar]
  21. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  23. Teather R. M., Wood P. J. ( 1982 ). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. . Appl Environ Microbiol 43, 777780.[PubMed]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;. [CrossRef]
    [Google Scholar]
  26. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  27. Xie C.-H., Yokota A. ( 2003 ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49, 345349. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yi H., Cho J.-C., Chun J. ( 2011 ). Pontirhabdus pectinivorans gen. nov., sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61, 24752481.[PubMed] [CrossRef]
    [Google Scholar]
  29. Yi H., Cho J.-C., Chun J. ( 2012 ). Flavivirga jejuensis gen. nov., sp. nov., and Flavivirga amylovorans sp. nov., new members of the family Flavobacteriaceae isolated from seawater, and emended descriptions of the genera Psychroserpens and Lacinutrix . . Int J Syst Evol Microbiol 62, 10611068. [CrossRef] [PubMed]
    [Google Scholar]
  30. Zhang Y., Tang K., Shi X., Zhang X. H. ( 2013 ). Flaviramulus ichthyoenteri sp. nov., an N-acylhomoserine lactone-degrading bacterium isolated from the intestine of a flounder (Paralichthys olivaceus), and emended descriptions of the genus Flaviramulus and Flaviramulus basaltis. . Int J Syst Evol Microbiol 63, 44774483. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000110
Loading
/content/journal/ijsem/10.1099/ijs.0.000110
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error