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Abstract

An actinobacterium strain (M-201) was isolated from a deep-sea scleractinian coral () collected at 1500 m depth in the Avilés Canyon in the Cantabrian Sea, Asturias, Spain. Strain M-201 grew at pH 6.0–9.0 (optimum pH 7.0), between 4 and 37 °C (optimum 28 °C) and at salinities of 0.5–10.5 % (w/v) NaCl (optimum 0.5–3.0 %). The peptidoglycan contained the amino acids Lys, Ala, Thr, Glu and one unknown amino acid component, and belonged to type A4α, and the cell-wall sugars are glucose, mannose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unknown phosphoglycolipid and seven unknown glycolipids. The predominant menaquinones were MK-9(H) and MK-9(H). Major cellular fatty acids were anteiso-C, iso-C and anteiso-C. The genomic DNA G+C content was 72.4 mol%. The chemotaxonomic properties supported the affiliation of strain M-201 to the genus . Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to CD12E2-27 (98.2 % 16S rRNA gene sequence similarity). However, it had a relatively low DNA–DNA relatedness value with the above strain (48 %). The isolate showed antibiotic activity against ATCC 14452 and var. carlsbergensis. To the best of our knowledge, this is the first report of antibiotic production in the genus . The differences in phenotypic, metabolic, ecological and phylogenetic characteristics justify the proposal of a novel species of the genus , sp. nov., with M-201 ( = CECT 8512 = DSM 28392) as the type strain.

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2015-04-01
2020-01-22
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