1887

Abstract

An aerobic halotolerant restricted facultatively methylotrophic bacterium was isolated from a saline hot spring in Pamukkale, Turkey, and designated strain PK2. The cells of this strain were Gram-stain-negative, asporogenous, motile short rods multiplying by binary fission. They utilized methanol, methylamine and mannitol as carbon and energy sources. The organism grew optimally at 30 °C in media containing 85 mM NaCl and at pH 7.5–8.0. C compounds were assimilated via the isocitrate-lyase-positive variant of the serine pathway. Poly-β-hydroxybutyrate and the compatible solute ectoine were found in the cells. The dominant phospholipids were phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The major cellular fatty acids of methanol-grown cells were Cω7 and Cω7. The main ubiquinone was Q-10. The DNA G+C content was 67.9 mol% ( ). The 16S rRNA gene sequence suggests that strain PK2 is affiliated with the order within the class , being most closely related to CCBAU 83330 (94 % similarity). A novel genus and species, gen. nov., sp. nov., is proposed on the basis of phenotypic and genotypic data, with PK2 (VKM B-2849 = JCM 30229) as the type strain.

Funding
This study was supported by the:
  • , Russian Science Foundation , (Award 14-14-01045)
  • , Russian Foundation for Basic Research , (Award 14-04-31352)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000105
2015-04-01
2021-02-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/4/1321.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000105&mimeType=html&fmt=ahah

References

  1. Boden R. ( 2012 ). Emended description of the genus Methylophaga Janvier et al. 1985. . Int J Syst Evol Microbiol 62, 16441646. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bradford M. M. ( 1976 ). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. . Anal Biochem 72, 248254. [CrossRef] [PubMed]
    [Google Scholar]
  3. Doronina N. V., Trotsenko Y. A., Tourova T. P. ( 2000 ). Methylarcula marina gen. nov., sp. nov. and Methylarcula terricola sp. nov.: novel aerobic, moderately halophilic, facultatively methylotrophic bacteria from coastal saline environments. . Int J Syst Evol Microbiol 50, 18491859.[PubMed] [CrossRef]
    [Google Scholar]
  4. Doronina N. V., Ezhov V. A., Beschastnyi A. P., Trotsenko Yu. A. ( 2010 ). Biosynthesis of the bioprotectant ectoine by aerobic methylotrophic bacteria from methanol. . Appl Biochem Microbiol 46, 173176. [CrossRef]
    [Google Scholar]
  5. Doronina N. V., Gogleva A. A., Trotsenko Y. A. ( 2012 ). Methylophilus glucosoxydans sp. nov., a restricted facultative methylotroph from rice rhizosphere. . Int J Syst Evol Microbiol 62, 196201. [CrossRef] [PubMed]
    [Google Scholar]
  6. Doronina N. V., Poroshina M. N., Kaparullina E. N., Ezhov V. A., Trotsenko Y. A. ( 2013 ). Methyloligella halotolerans gen. nov., sp. nov. and Methyloligella solikamskensis sp. nov., two non-pigmented halotolerant obligately methylotrophic bacteria isolated from the Ural saline environments. . Syst Appl Microbiol 36, 148154. [CrossRef] [PubMed]
    [Google Scholar]
  7. Doronina N. V., Kaparullina E. N., Trotsenko Y. A. ( 2014 ). Methyloversatilis thermotolerans sp. nov., a novel thermotolerant facultative methylotroph isolated from a hot spring. . Int J Syst Evol Microbiol 64, 158164. [CrossRef] [PubMed]
    [Google Scholar]
  8. Han T. X., Han L. L., Wu L. J., Chen W. F., Sui X. H., Gu J. G., Wang E. T., Chen W. X. ( 2008 ). Mesorhizobium gobiense sp. nov. and Mesorhizobium tarimense sp. nov., isolated from wild legumes growing in desert soils of Xinjiang, China. . Int J Syst Evol Microbiol 58, 26102618. [CrossRef] [PubMed]
    [Google Scholar]
  9. Hilyard E. J., Jones-Meehan J. M., Spargo B. J., Hill R. T. ( 2008 ). Enrichment, isolation, and phylogenetic identification of polycyclic aromatic hydrocarbon-degrading bacteria from Elizabeth River sediments. . Appl Environ Microbiol 74, 11761182. [CrossRef] [PubMed]
    [Google Scholar]
  10. Ivanova E. G., Doronina N. V., Trotsenko Y. A. ( 2007 ). Hansschlegelia plantiphila gen. nov. sp. nov., a new aerobic restricted facultative methylotrophic bacterium associated with plants. . Syst Appl Microbiol 30, 444452. [CrossRef] [PubMed]
    [Google Scholar]
  11. Janvier M., Frehel C., Grimont F., Gasser F. ( 1985 ). Methylophaga marina gen. nov., sp. nov. and Methylophaga thalassica sp. nov., marine methylotrophs. . Int J Syst Bacteriol 35, 131139. [CrossRef]
    [Google Scholar]
  12. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  13. Marmur J. A. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [CrossRef]
    [Google Scholar]
  14. Owen R. J., Lapage S. P. ( 1976 ). The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic applications. . J Appl Bacteriol 41, 335340. [CrossRef] [PubMed]
    [Google Scholar]
  15. Poroshina M. N., Doronina N. V., Kaparullina E. N., Kovalevskaya N. P., Trotsenko Yu. A. ( 2013 ). Halophilic and halotolerant aerobic methylobacteria from the technogenic Solikamsk biotopes. . Microbiology (English translation of Mikrobiologiya) 82, 490498. [CrossRef]
    [Google Scholar]
  16. Poroshina M. N., Doronina N. V., Ezhov V. A., Trotsenko Y. A. ( 2014 ). Comparative characteristics of biosynthesis of polyhydroxybutyrate from methanol by Methylobacteria extorquens G10 and Methyloligella halotolerans C2. . Appl Biochem Microbiol 50, 253258. [CrossRef]
    [Google Scholar]
  17. Sambrook J., Russell D. W. ( 2001 ). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, New York:: Cold Spring Harbor Laboratory Press;.
    [Google Scholar]
  18. Schäfer H. ( 2007 ). Isolation of Methylophaga spp. from marine dimethylsulfide-degrading enrichment cultures and identification of polypeptides induced during growth on dimethylsulfide. . Appl Environ Microbiol 73, 25802591. [CrossRef] [PubMed]
    [Google Scholar]
  19. Sorokin D. Y., Trotsenko Y. A., Doronina N. V., Tourova T. P., Galinski E. A., Kolganova T. V., Muyzer G. ( 2007 ). Methylohalomonas lacus gen. nov., sp. nov. and Methylonatrum kenyense gen. nov., sp. nov., methylotrophic gammaproteobacteria from hypersaline lakes. . Int J Syst Evol Microbiol 57, 27622769. [CrossRef] [PubMed]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  22. Trotsenko Y. A., Doronina N. V., Govorukhina N. I. ( 1986 ). Metabolism of non-motile obligately methylotrophic bacteria. . FEMS Microbiol Lett 33, 293297. [CrossRef]
    [Google Scholar]
  23. Vandecandelaere I., Segaert E., Mollica A., Faimali M., Vandamme P. ( 2008 ). Leisingera aquimarina sp. nov., isolated from a marine electroactive biofilm, and emended descriptions of Leisingera methylohalidivorans Schaefer et al. 2002, Phaeobacter daeponensis Yoon et al. 2007 and Phaeobacter inhibens Martens et al. 2006. . Int J Syst Evol Microbiol 58, 27882793. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000105
Loading
/content/journal/ijsem/10.1099/ijs.0.000105
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error