1887

Abstract

An aerobic halotolerant restricted facultatively methylotrophic bacterium was isolated from a saline hot spring in Pamukkale, Turkey, and designated strain PK2. The cells of this strain were Gram-stain-negative, asporogenous, motile short rods multiplying by binary fission. They utilized methanol, methylamine and mannitol as carbon and energy sources. The organism grew optimally at 30 °C in media containing 85 mM NaCl and at pH 7.5–8.0. C compounds were assimilated via the isocitrate-lyase-positive variant of the serine pathway. Poly-β-hydroxybutyrate and the compatible solute ectoine were found in the cells. The dominant phospholipids were phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The major cellular fatty acids of methanol-grown cells were Cω7 and Cω7. The main ubiquinone was Q-10. The DNA G+C content was 67.9 mol% ( ). The 16S rRNA gene sequence suggests that strain PK2 is affiliated with the order within the class , being most closely related to CCBAU 83330 (94 % similarity). A novel genus and species, gen. nov., sp. nov., is proposed on the basis of phenotypic and genotypic data, with PK2 (VKM B-2849 = JCM 30229) as the type strain.

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2015-04-01
2019-10-18
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