sp. nov., a diesel oildegrading and solventtolerant bacterium Free

Abstract

Two Gram-stain-positive, diesel oil-degrading, solvent-tolerant, aerobic, endospore-forming, rod-shaped bacteria were isolated from a contaminated laboratory plate. Based on 16S rRNA gene sequence analysis, strains JC228 and JC279 were identified as belonging to the genus within the family of the phylum and were found to be most closely related to JCM 9731 (98.1 % 16S rRNA gene sequence similarity) and shared <96.0 % 16S rRNA gene sequence similarity with other members of the genus . The DNA–DNA hybridization value between the two strains was 88±2 %. Strain JC228 showed 23.4±1 % reassociation (based on DNA–DNA hybridization) with LMG 18001. The DNA G+C content of strains JC228 and JC279 was 39 and 38.4 mol%, respectively. Both strains were positive for catalase and oxidase activities, and negative for hydrolysis of starch and Tween 80. Strains JC228 and JC279 grew chemoorganoheterotrophically with optimum growth at pH 7 (range pH 7–9.5) and 35 °C (range 25–40 °C). Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid (PL2) were the major polar lipids. Major cellular fatty acids were iso-C, anteiso-C, iso-C and C. Whole-cell hydrolysates contained -alanine, -alanine, -glutamic acid and -diaminopimelic acid. Both strains utilized diesel oil as sole carbon and energy source. The results of physiological, biochemical, chemotaxonomic and molecular analyses allowed clear differentiation of strains JC228 and JC279 from their closest phylogenetic neighbours. Therefore strains JC228 and JC279 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC228 ( = LMG 28084 = CCTCC AB 2013353).

Funding
This study was supported by the:
  • University Grants commission
  • Moulana Azad National Fellowship (MANF)
  • CSIR, New Delhi
  • SRF
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000103
2015-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/4/1310.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000103&mimeType=html&fmt=ahah

References

  1. Amoozegar M. A., Didari M., Bagheri M., Fazeli S. A. S. F., Schumann P., Spröer C., Sánchez-Porro C., Ventosa A. ( 2013 ). Bacillus salsus sp. nov., a halophilic bacterium from a hypersaline lake. . Int J Syst Evol Microbiol 63, 33243329. [View Article] [PubMed]
    [Google Scholar]
  2. Cappuccino J. G., Sherman N. ( 1998 ). Microbiology: a Laboratory Manual, , 5th edn.. Menlo Park, CA:: Benjamin/Cummings;.
    [Google Scholar]
  3. Carrasco I. J., Márquez M. C., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. ( 2007 ). Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China. . Int J Syst Evol Microbiol 57, 20842088. [View Article] [PubMed]
    [Google Scholar]
  4. Chakravarthy S. K., Ramaprasad E. V. V., Shobha E., Sasikala Ch., Ramana ChV. ( 2012 ). Rhodoplanes piscinae sp. nov. isolated from pond water. . Int J Syst Evol Microbiol 62, 28282834. [View Article] [PubMed]
    [Google Scholar]
  5. Cohn F. ( 1872 ). Untersuchungen über Bakterien. . Beitr Biol Pflanz 1, 127224 (in German).
    [Google Scholar]
  6. Kates M. ( 1972 ). Techniques of Lipidology. New York:: Elsevier;. [CrossRef]
    [Google Scholar]
  7. Kates M. ( 1986 ). Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids. Amsterdam:: Elsevier;.
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  9. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  10. Lakshmi K. V. N. S., Sasikala Ch., Ashok Kumar G. V., Chandrasekaran R., Ramana Ch. V. ( 2011 ). Phaeovibrio sulfidiphilus gen. nov., sp. nov., phototrophic alphaproteobacteria isolated from brackish water. . Int J Syst Evol Microbiol 61, 828833. [View Article] [PubMed]
    [Google Scholar]
  11. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. et al. ( 2009 ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59, 21142121. [View Article] [PubMed]
    [Google Scholar]
  12. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  13. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J. ( 2000 ). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. . Lett Appl Microbiol 30, 178182. [View Article] [PubMed]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  15. Oren A., Duker S., Ritter S. ( 1996 ). The polar lipid composition of Walsby’s square bacterium. . FEMS Microbiol Lett 138, 135140. [View Article]
    [Google Scholar]
  16. Priest F. G. ( 1993 ). Systematics and ecology of Bacillus . . In Bacillus subtilis and Other Gram-positive Bacteria, pp. 333. Edited by Sonenshein A. L., Hoch J. A., Losick R. . Washington, DC:: American Society for Microbiology;. [CrossRef]
    [Google Scholar]
  17. Priest F. G., Goodfellow M., Todd C. ( 1988 ). A numerical classification of the genus Bacillus . . J Gen Microbiol 134, 18471882.[PubMed]
    [Google Scholar]
  18. Ramos J. L., Duque E., Gallegos M. T., Godoy P., Ramos-Gonzalez M. I., Rojas A., Teran W., Segura A. ( 2002 ). Mechanisms of solvent tolerance in Gram-negative bacteria. . Annu Rev Microbiol 56, 743768. [View Article] [PubMed]
    [Google Scholar]
  19. Reddy S. V., Thirumala M., Farooq M., Sasikala Ch., Ramana Ch. V. ( 2015 ). Bacillus lonarensis sp. nov., an alkalitolerant bacterium isolated from a soda lake. . Arch Microbiol 197, 2734. [View Article] [PubMed]
    [Google Scholar]
  20. Rheims H., Frühling A., Schumann P., Rohde M., Stackebrandt E. ( 1999 ). Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall. . Int J Syst Bacteriol 49, 795802. [View Article] [PubMed]
    [Google Scholar]
  21. Sasser M. . ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  22. Schaeffer A. B., Fulton M. D. ( 1933 ). A Simplified method of staining endospores. . Science 77, 194. [View Article] [PubMed]
    [Google Scholar]
  23. Schleifer K. H. ( 1985 ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18, 123156. [View Article]
    [Google Scholar]
  24. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  25. Seldin L., Dubnau D. ( 1985 ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans and other nitrogen-fixing Bacillus strains. . Int J Syst Bacteriol 35, 151154. [View Article]
    [Google Scholar]
  26. Skerman V. B. D., Mcgowan V., Sneath P. H. A. (editors) ( 1980 ). Approved Lists of Bacterial Names. Washington, DC:: American Society for Microbiology;. [View Article]
    [Google Scholar]
  27. Slepecky R., Hemphill E. ( 2006 ). The genus Bacillus – nonmedical. . In The Prokaryotes, vol. 4, pp. 530562. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  28. Smibert R. M., Krieg N. R. ( 1981 ). General characterization. . In Manual of Methods for General Bacteriology, pp. 409443. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  29. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  30. Subhash Y., Sasikala Ch., Ramana Ch. V. ( 2014 ). Bacillus luteus sp. nov., isolated from soil. . Int J Syst Evol Microbiol 64, 15801586. [View Article] [PubMed]
    [Google Scholar]
  31. Takami H. ( 2006 ). Genomic diversity of extremophilic Gram-positive endospore-forming Bacillus-related species. . In Trends in Genome Research, pp. 2585. Edited by Williams C. R. . New York:: NOVA;.
    [Google Scholar]
  32. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013 ). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30, 27252729. [View Article] [PubMed]
    [Google Scholar]
  33. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  34. Tindall B. J., Tomlinson G. A., Hochstein L. I. ( 1987 ). Polar lipid composition of a new halobacterium. . Syst Appl Microbiol 9, 68. [View Article] [PubMed]
    [Google Scholar]
  35. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L. ( 2005 ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55, 11491153. [View Article] [PubMed]
    [Google Scholar]
  36. Zhai L., Liao T., Xue Y., Ma Y. ( 2012 ). Bacillus daliensis sp. nov., an alkaliphilic, Gram-positive bacterium isolated from a soda lake. . Int J Syst Evol Microbiol 62, 949953. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000103
Loading
/content/journal/ijsem/10.1099/ijs.0.000103
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed