Two Gram-stain-positive, diesel oil-degrading, solvent-tolerant, aerobic, endospore-forming, rod-shaped bacteria were isolated from a contaminated laboratory plate. Based on 16S rRNA gene sequence analysis, strains JC228T and JC279 were identified as belonging to the genus Bacillus within the family Bacillaceae of the phylum Firmicutes and were found to be most closely related to Bacillus carboniphilus JCM 9731T (98.1 % 16S rRNA gene sequence similarity) and shared <96.0 % 16S rRNA gene sequence similarity with other members of the genus Bacillus. The DNA–DNA hybridization value between the two strains was 88±2 %. Strain JC228T showed 23.4±1 % reassociation (based on DNA–DNA hybridization) with B. carboniphilus LMG 18001T. The DNA G+C content of strains JC228T and JC279 was 39 and 38.4 mol%, respectively. Both strains were positive for catalase and oxidase activities, and negative for hydrolysis of starch and Tween 80. Strains JC228T and JC279 grew chemoorganoheterotrophically with optimum growth at pH 7 (range pH 7–9.5) and 35 °C (range 25–40 °C). Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid (PL2) were the major polar lipids. Major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0 and C16 : 0. Whole-cell hydrolysates contained l-alanine, d-alanine, d-glutamic acid and meso-diaminopimelic acid. Both strains utilized diesel oil as sole carbon and energy source. The results of physiological, biochemical, chemotaxonomic and molecular analyses allowed clear differentiation of strains JC228T and JC279 from their closest phylogenetic neighbours. Therefore strains JC228T and JC279 represent a novel species of the genus Bacillus, for which the name Bacillus oleivorans sp. nov. is proposed. The type strain is JC228T ( = LMG 28084T = CCTCC AB 2013353T).
AmoozegarM. A.,
DidariM.,
BagheriM.,
FazeliS. A. S. F.,
SchumannP.,
SpröerC.,
Sánchez-PorroC.,
VentosaA.(2013).Bacillus salsus sp. nov., a halophilic bacterium from a hypersaline lake. . Int J Syst Evol Microbiol63, 3324–3329. [View Article][PubMed]
CarrascoI. J.,
MárquezM. C.,
XueY.,
MaY.,
CowanD. A.,
JonesB. E.,
GrantW. D.,
VentosaA.(2007).Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China. . Int J Syst Evol Microbiol57, 2084–2088. [View Article][PubMed]
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
LoganN. A.,
BergeO.,
BishopA. H.,
BusseH. J.,
De VosP.,
FritzeD.,
HeyndrickxM.,
KämpferP.,
RabinovitchL. et al.(2009). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol59, 2114–2121. [View Article][PubMed]
McKerrowJ.,
VaggS.,
McKinneyT.,
SeviourE. M.,
MaszenanA. M.,
BrooksP.,
SeviourR. J.(2000). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. . Lett Appl Microbiol30, 178–182. [View Article][PubMed]
MesbahM.,
PremachandranU.,
WhitmanW. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol39, 159–167. [View Article]
PriestF. G.(1993). Systematics and ecology of Bacillus
. . In Bacillus subtilis and Other Gram-positive Bacteria, pp. 3–33. Edited by
SonensheinA. L.,
HochJ. A.,
LosickR.
. Washington, DC:: American Society for Microbiology;. [CrossRef]
RheimsH.,
FrühlingA.,
SchumannP.,
RohdeM.,
StackebrandtE.(1999).Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall. . Int J Syst Bacteriol49, 795–802. [View Article][PubMed]
SkermanV. B. D.,
McgowanV.,
SneathP. H. A.(editors) (1980).Approved Lists of Bacterial Names. Washington, DC:: American Society for Microbiology;. [View Article]
SlepeckyR.,
HemphillE.(2006). The genus Bacillus – nonmedical. . In The Prokaryotes, vol. 4, pp. 530–562. Edited by
DworkinM.,
FalkowS.,
RosenbergE.,
SchleiferK.-H.,
StackebrandtE.
. New York:: Springer;. [View Article]
SmibertR. M.,
KriegN. R.(1981). General characterization. . In Manual of Methods for General Bacteriology, pp. 409–443. Edited by
GerhardtP.,
MurrayR. G. E.,
CostilowR. N.,
NesterE. W.,
WoodW. A.,
KriegN. R.,
PhillipsG. B.
. Washington, DC:: American Society for Microbiology;.
StackebrandtE.,
GoebelB. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol44, 846–849. [View Article]
TakamiH.(2006). Genomic diversity of extremophilic Gram-positive endospore-forming Bacillus-related species. . In Trends in Genome Research, pp. 25–85. Edited by
WilliamsC. R.
. New York:: NOVA;.
TindallB. J.,
TomlinsonG. A.,
HochsteinL. I.(1987). Polar lipid composition of a new halobacterium. . Syst Appl Microbiol9, 6–8. [View Article][PubMed]
XuP.,
LiW. J.,
TangS. K.,
ZhangY. Q.,
ChenG. Z.,
ChenH. H.,
XuL. H.,
JiangC. L.(2005).Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol55, 1149–1153. [View Article][PubMed]
ZhaiL.,
LiaoT.,
XueY.,
MaY.(2012).Bacillus daliensis sp. nov., an alkaliphilic, Gram-positive bacterium isolated from a soda lake. . Int J Syst Evol Microbiol62, 949–953. [View Article][PubMed]