@article{mbs:/content/journal/ijsem/10.1099/ijs.0.000101, author = "Kaewwichian, Rungluk and Jindamorakot, Sasitorn and Am-In, Somjit and Sipiczki, Matthias and Limtong, Savitree", title = "Hannaella siamensis sp. nov. and Hannaella phetchabunensis sp. nov., two new anamorphic basidiomycetous yeast species isolated from plants", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_4", pages = "1297-1303", doi = "https://doi.org/10.1099/ijs.0.000101", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000101", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Eight strains, representing two novel anamorphic yeast species, consisted of five strains isolated from the external surfaces of rice leaves (DMKU-RP72T, DMKU-RP109, DMKU-RP119, YE-124 and YE-156) and one from a corn leaf (DMKU-CP430T)4 collected in Thailand, and one strain isolated from each of a composite flower (11-1114) and a fallen dead leaf (12-301); the latter two were collected in Belize. On the basis of sequence analysis of the D1/D2 region of the large subunit rRNA gene and the internal transcribed spacer (ITS) region, they were suggested to be two novel species of the genus Hannaella. Seven strains (DMKU-RP72T, DMKU-RP109, DMKU-RP119, YE-124, YE-156, 11-1114 and 12-301) differed from each other by 0–3 nt substitutions in the D1/D2 region and by 0–1 nt substitutions in the ITS region. In terms of pairwise sequence similarities of the D1/D2 region these seven strains were closest to Hannaella zeae, but with 1.2–1.7 % (7–9) nucleotide substitutions. The sequences of the ITS region of these seven strains differed from H. zeae by 3.7–3.9 % (16–17) nucleotide substitutions. Therefore, they were assigned to a single novel species and the name Hannaella siamensis sp. nov. has been proposed. The type strain is DMKU-RP72T ( = BCC 69493T = NBRC 110425T = CBS 13533T). Strain DMKU-CP430T represents the second novel species and was also most closely related to H. zeae, but with 1.0 % (6) nucleotide substitutions in the D1/D2 region and 3.2 % (14) nucleotide substitutions in the ITS region. It was assigned to the proposed novel species, Hannaella phetchabunensis sp. nov. (type strain DMKU-CP430T = BCC 69492T = NBRC 110424T = CBS 13386T).", }