1887

Abstract

Eight strains, representing two novel anamorphic yeast species, consisted of five strains isolated from the external surfaces of rice leaves (DMKU-RP72, DMKU-RP109, DMKU-RP119, YE-124 and YE-156) and one from a corn leaf (DMKU-CP430)4 collected in Thailand, and one strain isolated from each of a composite flower (11-1114) and a fallen dead leaf (12-301); the latter two were collected in Belize. On the basis of sequence analysis of the D1/D2 region of the large subunit rRNA gene and the internal transcribed spacer (ITS) region, they were suggested to be two novel species of the genus . Seven strains (DMKU-RP72, DMKU-RP109, DMKU-RP119, YE-124, YE-156, 11-1114 and 12-301) differed from each other by 0–3 nt substitutions in the D1/D2 region and by 0–1 nt substitutions in the ITS region. In terms of pairwise sequence similarities of the D1/D2 region these seven strains were closest to , but with 1.2–1.7 % (7–9) nucleotide substitutions. The sequences of the ITS region of these seven strains differed from by 3.7–3.9 % (16–17) nucleotide substitutions. Therefore, they were assigned to a single novel species and the name sp. nov. has been proposed. The type strain is DMKU-RP72 ( = BCC 69493 = NBRC 110425 = CBS 13533). Strain DMKU-CP430 represents the second novel species and was also most closely related to , but with 1.0 % (6) nucleotide substitutions in the D1/D2 region and 3.2 % (14) nucleotide substitutions in the ITS region. It was assigned to the proposed novel species, sp. nov. (type strain DMKU-CP430 = BCC 69492 = NBRC 110424 = CBS 13386).

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2015-04-01
2019-10-15
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