1887

Abstract

Two yeast strains representing two novel yeast species were isolated from frass of an unidentified insect (ST-78) and the external surfaces of rice leaves (YE170) collected in Thailand. The two strains were genetically, morphologically and phenotypically distinct from recognized species and were found to represent two novel species of the genus although formation of ascospores was not observed. In terms of pairwise sequence similarity of the D1/D2 region of the large subunit rRNA gene, the closest relative of strain ST-78 was CBS 9941 but with 3.8 % nucleotide substitutions, while the closest relative of strain YE170 was strain ST-78 but with 4.3 % nucleotide substitutions. Analysis of the sequences of the internal transcribed spacer 1 and 2 (ITS1–2) regions revealed that strain ST-78 differed from CBS 9941 by 8.8 % nucleotide substitutions and from strain YE170 by 9.4 % nucleotide substitutions. The result of pairwise sequence similarity of the D1/D2 and ITS1–2 regions together with phylogenetic analysis indicated that strains ST-78 and YE170 represented two novel species within the clade. The names f.a., sp. nov. (type strain ST-78 = BCC 8314 = NBRC 110421 = CBS 13382; MycoBank no. MB810546) and f.a., sp. nov. (type strain YE170 = BCC 63466 = NBRC 110423 = CBS 13384; MycoBank no. MB810547) are proposed for the two novel yeast species.

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2015-04-01
2019-12-08
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