1887

Abstract

A hyperthermophilic methanogen, strain JH146, was isolated from 26 °C hydrothermal vent fluid emanating from a crack in basaltic rock at Marker 113 vent, Axial Seamount in the northeastern Pacific Ocean. It was identified as an obligate anaerobe that uses only H and CO for growth. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain is more than 97 % similar to other species of the genus . Therefore, overall genome relatedness index analyses were performed to establish that strain JH146 represents a novel species. For each analysis, strain JH146 was most similar to FS406-22, which can fix N and also comes from Marker 113 vent. However, strain JH146 differs from strain FS406-22 in that it cannot fix N. The average nucleotide identity score for strain JH146 was 87 %, the genome-to-genome direct comparison score was 33–55 % and the species identification score was 93 %. For each analysis, strain JH146 was below the species delineation cut-off. Full-genome gene synteny analysis showed that strain JH146 and strain FS406-22 have 97 % genome synteny, but strain JH146 was missing the operons necessary for N fixation and assimilatory nitrate reduction that are present in strain FS406-22. Based on its whole genome sequence, strain JH146 is suggested to represent a novel species of the genus for which the name is proposed. The type strain is JH146 ( = DSM 27223 = KACC 18232).

Funding
This study was supported by the:
  • , Gordon and Betty Moore Foundation , (Award GBMF 3297)
  • , National Research Foundation of Korea , (Award MEST 2014-002744)
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2015-04-01
2020-10-24
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