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Volume 65, Issue Pt_4

sp. nov., isolated from tortoise () Free

Abstract

A polyphasic taxonomic analysis was carried out on 11 uncommon Gram-stain-negative, non-motile, catalase- and oxidase-positive, but indole-negative, bacterial strains isolated from tortoises. Phenotypically and genetically they represented a homogeneous group of organisms most closely related to, but distinct from, . In a reconstructed 16S rRNA gene tree they clustered on a monophyletic branch next to with gene similarities between strains of 99.5–100 %, and of up to 98.2 % with . DNA–DNA hybridization indicated the organisms represented a novel species with only 40 % DNA-DNA similarity with . Partial sequencing of resulted in two subclusters confirming the 16S rRNA gene phylogeny; both genes allowed clear separation and identification of the novel species. Furthermore, they could be unambiguously identified by matrix-assisted laser desorption ionization time-of-flight MS, where, again, they formed a highly homogeneous cluster separate from and other members of the family . The major fatty acids were C and summed feature Cω7/iso-C 2-OH. The DNA G+C content was 54.4 mol%. Based on phenotypic and genetic data we propose classifying these organisms as representatives of a novel species named sp. nov. The type strain is 07_OD624 ( = DSM 26510 = CCUG 63373).

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2015-04-01
2024-04-23
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References

  1. Adeolu M., Gupta R. S. ( 2013 ). Phylogenomics and molecular signatures for the order Neisseriales: proposal for division of the order Neisseriales into the emended family Neisseriaceae and Chromobacteriaceae fam. nov.. Antonie van Leeuwenhoek 104, 1–24. [View Article] [PubMed]
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461–466. [View Article] [PubMed]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  4. Dousse F., Thomann A., Brodard I., Korczak B. M., Schlatter Y., Kuhnert P., Miserez R., Frey J. ( 2008 ). Routine phenotypic identification of bacterial species of the family Pasteurellaceae isolated from animals. . J Vet Diagn Invest 20, 716–724. [View Article] [PubMed]
    [Google Scholar]
  5. Euzéby J. P. ( 1997 ). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47, 590–592. [View Article] [PubMed]
    [Google Scholar]
  6. Huß V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 989–1005. [View Article]
    [Google Scholar]
  8. Korczak B., Christensen H., Emler S., Frey J., Kuhnert P. ( 2004 ). Phylogeny of the family Pasteurellaceae based on rpoB sequences. . Int J Syst Evol Microbiol 54, 1393–1399. [View Article] [PubMed]
    [Google Scholar]
  9. Korczak B. M., Bisgaard M., Christensen H., Kuhnert P. ( 2014 ). Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds. . Antonie van Leeuwenhoek 105, 731–741. [View Article] [PubMed]
    [Google Scholar]
  10. Kuhnert P., Frey J., Lang N. P., Mayfield L. ( 2002 ). Phylogenetic analysis of Prevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis clinical strains reveals a clear species clustering. . Int J Syst Evol Microbiol 52, 1391–1395. [View Article] [PubMed]
    [Google Scholar]
  11. Kuhnert P., Bisgaard M., Korczak B. M., Schwendener S., Christensen H., Frey J. ( 2012 ). Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry. . J Microbiol Methods 89, 1–7. [View Article] [PubMed]
    [Google Scholar]
  12. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358–361. [View Article]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  14. Miller L. T. ( 1982 ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16, 584–586.[PubMed]
    [Google Scholar]
  15. Parte A. C. ( 2014 ). LPSN–list of prokaryotic names with standing in nomenclature. . Nucleic Acids Res 42 (Database issue), D613–D616. [View Article] [PubMed]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  17. Silipo A., Sturiale L., De Castro C., Lanzetta R., Parrilli M., Garozzo D., Molinaro A. ( 2012 ). Structure of the lipopolysaccharide isolated from the novel species Uruburuella suis . . Carbohydr Res 357, 75–82. [View Article] [PubMed]
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  19. Vela A. I., Collins M. D., Lawson P. A., García N., Domínguez L., Fernández-Garayzábal J. F. ( 2005 ). Uruburuella suis gen. nov., sp. nov., isolated from clinical specimens of pigs. . Int J Syst Evol Microbiol 55, 643–647. [View Article] [PubMed]
    [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
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Uruburuella testudinis sp. nov., isolated from tortoise (Testudo)
Int J Syst Evol Microbiol 65, 1251 (2015); https://doi.org/10.1099/ijs.0.000089
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