1887

Abstract

A polyphasic taxonomic analysis was carried out on 11 uncommon Gram-stain-negative, non-motile, catalase- and oxidase-positive, but indole-negative, bacterial strains isolated from tortoises. Phenotypically and genetically they represented a homogeneous group of organisms most closely related to, but distinct from, . In a reconstructed 16S rRNA gene tree they clustered on a monophyletic branch next to with gene similarities between strains of 99.5–100 %, and of up to 98.2 % with . DNA–DNA hybridization indicated the organisms represented a novel species with only 40 % DNA-DNA similarity with . Partial sequencing of resulted in two subclusters confirming the 16S rRNA gene phylogeny; both genes allowed clear separation and identification of the novel species. Furthermore, they could be unambiguously identified by matrix-assisted laser desorption ionization time-of-flight MS, where, again, they formed a highly homogeneous cluster separate from and other members of the family . The major fatty acids were C and summed feature Cω7/iso-C 2-OH. The DNA G+C content was 54.4 mol%. Based on phenotypic and genetic data we propose classifying these organisms as representatives of a novel species named sp. nov. The type strain is 07_OD624 ( = DSM 26510 = CCUG 63373).

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2015-04-01
2019-12-08
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