RT Journal Article SR Electronic(1) A1 Du, Juan A1 Singh, Hina A1 Ngo, Hien T. T. A1 Won, Kyung-Hwa A1 Kim, Ki-Young A1 Yi, Tae-HooYR 2015 T1 Pedobacter daejeonensis sp. nov. and Pedobacter trunci sp. nov., isolated from an ancient tree trunk, and emended description of the genus Pedobacter JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_4 SP 1241 OP 1246 DO https://doi.org/10.1099/ijs.0.000087 PB Microbiology Society, SN 1466-5034, AB Two Gram-stain-negative, yellow, aerobic and rod-shaped bacterial isolates, designated THG-DN3.18T and THG-DN3.19T, were isolated from an ancient tree trunk from Daejeon, South Korea. 16S rRNA gene sequence similarity showed that both strains belong to the genus Pedobacter within the family Sphingobacteriaceae . Strain THG-DN3.18T exhibited maximum sequence similarity with Pedobacter boryungensis KCTC 23344T (98.5 %) while strain THG-DN3.19T exhibited maximum sequence similarity with Pedobacter nyackensis LMG 24260T (97.3 %). In DNA–DNA hybridization tests, the two strains showed less than 35 % relatedness with respect to closely related species of the genus Pedobacter . Both strains contained iso-C15 : 0 and C16 : 1ω6c and/or C16 : 1ω7c (summed feature 3) as the predominant fatty acids and MK-7 as the major isoprenoid quinone. The DNA G+C contents of strains THG-DN3.18T and THG-DN3.19T were 35.5 and 40.1 mol%, respectively. The genotypic analysis, biochemical properties, and phenotypic and chemotaxonomic characteristics indicate that strains THG-DN3.18T and THG-DN3.19T represent novel species of the genus Pedobacter , for which the names Pedobacter daejeonensis sp. nov. and Pedobacter trunci sp. nov. are proposed. The type strains are THG-DN3.18T ( = KCTC 42230T = JCM 30352T) and THG-DN3.19T ( = KCTC 42233T = JCM 30353T), respectively., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000087