1887

Abstract

A Gram-stain-negative, rod-shaped, non-motile, aerobic bacterium, strain JL351, was isolated from the surface seawater of the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain had a close relationship with members of the genera and , and the closest relative was CL-GR66 (with 96.56 % 16S rRNA gene sequence similarity). The polar lipids of strain JL351 comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, three unidentified aminolipids, three unidentified phospholipids and an unidentified glycolipid. The predominant isoprenoid quinone was Q-10. The major fatty acids were Cω7 (60.9 %), C (13.7 %), C (9.4 %), 11-methyl Cω7 (4.5 %), and C 3-OH (4.4 %). The DNA G+C content was 66.2 mol%. Based on phenotypic, phylogenetic and genotypic data, strain JL351 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JL351 ( = CGMCC 1.12986 = JCM 30379).

Funding
This study was supported by the:
  • National Key Basic Research Program of China (Award 2013CB955700)
  • SOA Project (Award GASI-03-01-02-05)
  • National Natural Science Foundation of China (Award 41276131)
  • Natural Science Foundation of Fujian Province (Award 2014J01164)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000086
2015-04-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/4/1247.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000086&mimeType=html&fmt=ahah

References

  1. Buchan A., González J. M., Moran M. A. ( 2005 ). Overview of the marine Roseobacter lineage. . Appl Environ Microbiol 71, 56655677. [View Article] [PubMed]
    [Google Scholar]
  2. Dong X.-Z., Cai M.-Y. ( 2001 ). Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria. pp. 353412. Beijing:: Scientific Press; (in Chinese).
    [Google Scholar]
  3. Embley T. M. ( 1991 ). The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. . Lett Appl Microbiol 13, 171174. [View Article] [PubMed]
    [Google Scholar]
  4. Fraser S. L., Jorgensen J. H. ( 1997 ). Reappraisal of the antimicrobial susceptibilities of Chryseobacterium and Flavobacterium species and methods for reliable susceptibility testing. . Antimicrob Agents Chemother 41, 27382741.[PubMed]
    [Google Scholar]
  5. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and MolecularBbacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  6. González J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B. ( 1997 ). Sagittula stellata gen. nov., sp. nov., a lignin-transforming bacterium from a coastal environment. . Int J Syst Bacteriol 47, 773780. [View Article] [PubMed]
    [Google Scholar]
  7. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  8. Hwang C. Y., Cho B. C. ( 2008 ). Ponticoccus litoralis gen. nov., sp. nov., a marine bacterium in the family Rhodobacteraceae . . Int J Syst Evol Microbiol 58, 13321338. [View Article] [PubMed]
    [Google Scholar]
  9. Kates, M. (1972). Techniques of Lipidology: Isolation, Analysis and Identification of Lipids. Amsterdam:: Elsevier;. [View Article]
    [Google Scholar]
  10. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  11. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Braker G., Hirsch P. ( 1998 ). Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho Lake. . Int J Syst Bacteriol 48, 13631372. [View Article] [PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  13. Ostle A. G., Holt J. G. ( 1982 ). Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. . Appl Environ Microbiol 44, 238241.[PubMed]
    [Google Scholar]
  14. Rüger H.-J., Krambeck H.-J. ( 1994 ). Evaluation of the BIOLOG substrate metabolism system for classification of marine bacteria. . Syst Appl Microbiol 17, 281288. [View Article]
    [Google Scholar]
  15. Sasser M. . ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  16. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  18. Tang K., Huang H., Jiao N., Wu C. H. ( 2010 ). Phylogenomic analysis of marine Roseobacters . . PLoS ONE 5, e11604. [View Article] [PubMed]
    [Google Scholar]
  19. Tindall B. J. ( 1990 a). A comparative study of the lipid composition of Halobacterim saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [View Article]
    [Google Scholar]
  20. Tindall B. J. ( 1990 b). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  21. Wagner-Döbler I., Biebl H. ( 2006 ). Environmental biology of the marine Roseobacter lineage. . Annu Rev Microbiol 60, 255280. [View Article] [PubMed]
    [Google Scholar]
  22. Woese C. R., Stackebrandt E., Weisburg W. G., Paster B. J., Madigan M. T., Fowler V. J., Hahn C. M., Blanz P., Gupta R. et al. ( 1984 ). The phylogeny of purple bacteria: the alpha subdivision. . Syst Appl Microbiol 5, 315326. [View Article] [PubMed]
    [Google Scholar]
  23. Yurkov V. V., Krieger S., Stackebrandt E., Beatty J. T. ( 1999 ). Citromicrobium bathyomarinum, a novel aerobic bacterium isolated from deep-sea hydrothermal vent plume waters that contains photosynthetic pigment-protein complexes. . J Bacteriol 181, 45174525.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000086
Loading
/content/journal/ijsem/10.1099/ijs.0.000086
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error